Abstract
Neurotransmitter uptake into synaptic vesicles is mediated by vesicular neurotransmitter transporters. Although these transporters belong to different families, they all are thought to share a common overall topology with an even number of transmembrane domains. Using epitope-specific antibodies and mass spectrometry we show that the vesicular GABA transporter (VGAT) possesses an uneven number of transmembrane domains, with the N terminus facing the cytoplasm and the C terminus residing in the synaptic vesicle lumen. Antibodies recognizing the C terminus of VGAT (anti-VGAT-C) selectively label GABAergic nerve terminals of live cultured hippocampal and striatal neurons as confirmed by immunocytochemistry and patch-clamp electrophysiology. Injection of fluorochromated anti-VGAT-C into the hippocampus of mice results in specific labeling of GABAergic synapses in vivo. Overall, our data open the possibility of studying novel GABA release sites, characterizing inhibitory vesicle trafficking, and establishing their contribution to inhibitory neurotransmission at identified GABAergic synapses.
- endocytosis
- fluorescence detection
- synaptic plasticity
- vesicular GABA transporter
- live cell imaging
- synaptic vesicle
Introduction
Quantal release of neurotransmitters, concentrated in synaptic vesicles (SVs) (Sudhof, 2004) undergoing activity-dependent exocytosis at synaptic active zones (Schoch and Gundelfinger, 2006), subserves neuronal communication at every synapse. Release of a neurotransmitter from SVs requires either a complete SV fusion and subsequent endocytosis recovering the vesicle membrane (Jahn, 1999), or a “kiss-and-run” mechanism with a transiently open SV fusion pore allowing neurotransmitter release into the synaptic cleft (Klingauf et al., 1998).
Vesicular neurotransmitter transporters (VNTs) shuttle neurotransmitters from the cytosol into SVs (Gasnier, 2000; Chaudhry et al., 2008a). VNTs, including those of glutamate (VGluT1–3) (Takamori, 2006), acetylcholine (VAChT) (Erickson et al., 1996a), monoamines (VMATs) (Erickson et al. 1996b; Liu and Edwards, 1997), and glycine/GABA (VGAT/VIAAT) (Chaudhry et al. 1998) exhibit characteristic substrate specificities and are often used as phenotypic neuronal markers (Takamori et al. 2000). Understanding the correct structure of VNTs is imperative to deduce their functional domains, to define their physicochemical properties, and to identify their domains transiently exposed on the plasmalemmal surface during SV fusion. Our knowledge on the transmembrane topology of VNTs is largely based on computer predictions, which propose cytosolic localization for both the N and C termini of all VNTs and an even number (6–12) of transmembrane domains (McIntire et al., 1997; Masson et al., 1999). Experimental data on VGAT partly support the model predictions by showing that GABAergic SVs can be selectively immunoisolated with antibodies raised against its cytosolic N-terminus extremity (Takamori et al., 2000).
To further resolve the transmembrane topology of VGAT we applied limited proteolysis, a method successfully used to map the topologies of other SV proteins (synaptophysin, synaptogyrin, synaptotagmin-1 [Syt1]). Accordingly, luminal epitopes, e.g., the N terminus of Syt1 (Perin et al., 1991), are resistant to proteolytic cleavage as revealed by the molecular sizes of protected fragments by Western blotting (Stenius et al., 1995). In contrast, epitopes localized to the outer SV surface facing the cytosol, e.g., the N- and C-terminal endings of synaptogyrin and synaptophysin, are degradable. Here, we demonstrate that the C terminus of VGAT folds into the SV lumen. We also developed antibodies against short peptide sequences in (1) the N terminus, (2) the C terminus, or (3) the first luminal loop of VGAT (VGAT-N, VGAT-C, VGAT-lum1, respectively) (see Fig. 1) to elucidate the membrane topology of this protein, and to visualize forebrain GABAergic synapses both in vitro and in vivo. Similar to antibodies specific for the luminal domain of Syt1, which access the epitope during SV fusion and label endocytic vesicles (Kraszewski et al., 1995), fluorescent anti-VGAT-C targeting an intravesicular epitope allows the selective labeling of inhibitory presynaptic SV clusters during synaptic activity. Patch-clamp electrophysiology supports that anti-VGAT-C selectively recognizes VGAT. Collectively, our data elucidate a refined VGAT transmembrane topology and show that selective targeting of intraluminal VNT epitopes provides novel, effective means to label synapses of living neurons to establish their neurotransmitter phenotype in vivo.
Materials and Methods
Topology predictions.
Transmembrane domain predictions were performed using default settings of the TMHMM (Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark; http://www.cbs.dtu.dk/services/TMHMM) and HMMTOP algorithms (Hungarian Academy of Sciences, http://www.enzim.hu/hmmtop/), and the Tmpred service (http://www.ch.embnet.org/software/TMPRED_form.html). Models were compared with the structure reported by McIntire et al. (1997). Theoretical molecular weights of proteins and proteolytic fragments were calculated with the ProtParam tool (www.expasy.org; Swiss Institute of Bioinformatics, Basel, Switzerland).
Antibodies and fluorochromation.
Commercially available antibodies are listed in supplemental material, available at www.jneurosci.org. Peptides corresponding to AA 510–525 (anti-VGAT-C) and AA 171–184 (anti-VGAT-lum1) of rat VGAT (McIntire et al., 1997) were synthesized and coupled to keyhole limpet hemocyanin using standard procedures (Schneider et al., 1983). Antibodies were custom-generated (Biogenes) and affinity-purified using antigenic peptides immobilized on Sulfo-Link-Sepharose (Perbio) and are available from Synaptic Systems. Fluorochromation of affinity-purified anti-VGAT-C was performed following the manufacturers protocols (see supplemental material, available at www.jneurosci.org).
Biochemistry.
Fractions enriched in synaptic vesicles (LP2) and pure SVs were prepared from adult Wistar rat brains according to Nagy et al. (1976). SDS-PAGE was used to detect full-length proteins (Laemmli, 1970). Pronase-digested SVs were separated by tricine SDS-PAGE to improve resolution in the low molecular range (Schägger and von Jagow, 1987). Each sample contained 200 μg of LP2 or 100 μg of pronase-treated SVs. Purified SVs (1 mg) were proteolytically digested with 200 μg of pronase (Sigma) in 2.5 ml of PBS (0.05 m, pH 7.4) for 3 h. Reactions were terminated by boiling the samples in SDS sample buffer containing 3% β-mercaptoethanol. Western blotting was performed according to Towbin et al. (1979). Mass spectrometry is described in supplemental material, available at www.jneurosci.org.
Immunoperoxidase staining.
Immunoperoxidase staining of free-floating Vibratome sections was performed according to Chaudhry et al. (1998) (supplemental material, available at www.jneurosci.org).
Cell culturing and VGAT uptake.
Primary hippocampal neurons were cultured according to Gauthier-Campbell et al. (2004). Internalization of fluorochromated antibodies was initiated by incubating neurons in Krebs-Ringer solution containing 55 mm KCl (Kraszewski et al., 1995) and polyclonal anti-VGAT-N, anti-VGAT-C or anti-VGAT-lum1 antibodies (5 μg/ml) at 37°C for 5 min. Cells were repeatedly washed in Krebs-Ringer buffer and subsequently in PBS. Images showing primary fluorochromated antibody binding were captured immediately. Unlabeled primary antibodies were detected after immersion fixation in 4% paraformaldehyde in PBS, blocking and permeabilization with PBS containing 3% BSA and 0.1% Triton X-100 for 20 min, and exposure to Cy3-tagged goat anti-rabbit IgG (1 h, 10–20 μg/ml, Jackson ImmunoResearch). Specific labeling was confirmed by co-staining with monoclonal anti-VGAT-N and Cy5-conjugated goat anti-mouse IgG. Details of CypHer5E-anti-VGAT-C uptake are in supplemental material, available at www.jneurosci.org.
Electrophysiology.
Whole-cell patch-clamp recordings in hippocampal and striatal island cultures prepared from P0 mice were made according to Pyott and Rosenmund (2002) (supplemental material, available at www.jneurosci.org).
In vivo labeling.
GABAergic terminals were labeled by stereotaxic injection of anti-VGAT-C into the hippocampi of anesthetized (33 μg/g; Hypnomidate, Janssen) young adult C57BL/6N mice. Surgery was performed as described (Siegemund et al., 2006) with 4 μg/2 μl anti-VGAT-C antibody injected at coordinates: AP = −2.2 mm, L = 1.9 mm, DV = 1.75 mm (relative to bregma). Mice were transcardially perfused 48 h later, and tissues processed according to Siegemund et al. (2006). In vivo labeling was analyzed in 30-μm-thick frozen sections as previously described for cholinergic neurons (Härtig et al., 1998). Labeling specificity was assessed by colocalization of Cy3- or Oyster550-anti-VGAT-C (2 μg/ml) and glutamate decarboxylase (GAD; 1:500), VGAT-N (1:50), parvalbumin (1:300) or synaptophysin (1:500; monoclonal IgGs). The absence of nonspecific in vivo labeling of glutamatergic fibers was shown by using guinea pig-anti-VGLUT1 (1:500; supplemental Table 1, available at www.jneurosci.org as supplemental material).
Results
Refined VGAT topology and verification by pronase mapping and mass spectrometry
McIntire et al. (1997) initially suggested a transmembrane VGAT structure with both termini facing the cytoplasm (Fig. 1A). Based on the TMHMM algorithm, we predicted an alternative transmembrane topology with the C terminus residing in the SV lumen being transiently exposed on the extracellular plasmalemmal surface during exocytotic fusion of SVs (Fig. 1B). We verified the transmembrane structure of VGAT by protease treatment of SVs followed by immunoblot analysis of the resulting fragments using epitope-mapped VGAT-N, VGAT-C and VGAT-lum1 antibodies (Fig. 1). Luminal, N- and cytoplasmic C-terminal tails of Syt1, and the C termini of VGLUT1, VGLUT2 and VAChT served as controls. Specificity of antibodies directed against three different epitopes of VGAT was confirmed by the findings that they recognized identical bands on cultured wild-type neurons but not on VGAT−/− material (Fig. 2A). Antibodies against VGLUT1, VGLUT2, VAChT and Syt1 revealed bands at the predicted molecular sizes (Perin et al., 1991, Stenius et al., 1995, Takamori et al., 2000) in fractions enriched in synaptic vesicles (LP2) (Fig. 2B). Pronase treatment led to the loss of the VGAT-N epitope (Fig. 2C). In contrast, probing of blots with anti-VGAT-C (AA 510–525) and anti-VGAT-lum1 (AA171–184) yielded distinct bands showing that the epitopes were protected against proteolysis within the SV lumen (Fig. 2C). Because pronase treatment degrades all regions exposed on the vesicle surface, our refined topology model (Fig. 1B) predicts peptide fragments of 10.4 kDa and 3.9 kDa for the anti-VGAT-lum1 and anti-VGAT-C, respectively. Indeed, anti-VGAT-lum1 detected a proteolytic fragment of the expected size. However, the major protected C-terminal fragment migrated as a ∼12 kDa protein while only a weaker signal of the expected size was detectable. No signals were obtained after pronase treatment with antibodies directed against the C termini of VGLUT1, VGLUT2 and VAChT, in agreement with their predicted cytoplasmic localization.
Refined transmembrane topology of VGAT. A, Transmembrane structure of VGAT as proposed by McIntire et al. (1997) and as predicted by the TMHMM algorithm (B). Transmembrane domains are marked in gray. Epitopes in green were used to produce antibodies. Epitopes in red were detected by mass spectrometry. Insets denote the predicted exposure of luminal VGAT epitopes at the plasmalemmal surface and the likelihood of their targeting by antibodies during SV recycling.
Epitope mapping of VGAT using domain-specific antibodies and limited SV proteoloysis. A, Comparison of antibodies raised against the N (VGAT-N) and C termini (VGAT-C), and the first luminal domain (VGAT-lum1) of VGAT by Western blotting of cell lysates from cultured wild-type (wt) and VGAT−/− hippocampal neurons. B, Western analysis of enriched SVs (LP2) with antibodies recognizing the C termini of VGLUT1, VGLUT2, VAChT, the luminal N terminus (Syt1-N) and the cytoplasmic C terminus (Syt1-C) of Syt1. C, Western analysis of proteolytic fragments (arrowhead) after pronase treatment of SVs. D–G, Immunoperoxidase staining of VGAT-N and -C in hippocampal sections when luminal epitopes are concealed from or exposed to antibodies. Punctate terminal-like staining was detected around unstained pyramidal cells (Py) in the CA1 region by anti-VGAT-N both in the absence and presence of a detergent, respectively (arrowheads) (D, F). Under identical staining conditions, anti-VGAT-C failed to reveal any terminal-like staining in the absence of a detergent even at high antibody concentrations causing aspecific nuclear staining (arrows) (E). Immunoperoxidase staining for VGAT-C with Triton X-100, which solubilizes membranes and facilitates antibody penetration into SV lumen, shows a punctate staining pattern resembling that obtained by anti-VGAT-N (G). Scale bar, 25 μm.
To confirm that intravesicular epitopes remained protected during pronase treatment we monitored the cleavage of Syt1 using antibodies specific for the N-terminal intraluminal and C-terminal cytoplasmic domains, respectively. While the cytoplasmic epitope was lost, a fragment of the expected size was detected by the N-terminal antibody (Fig. 2C). Furthermore, trypsin treatment coupled with mass spectrometry of digested fragments revealed four additional cytosolic peptide fragments (supplemental Fig. 1, available at www.jneurosci.org as supplemental material). Three of these fragments covered the cytosolic N terminus while one corresponds to the third cytoplasmic loop (Fig. 1B; supplemental Fig. 1, available at www.jneurosci.org as supplemental material) thus lending further support to our refined model of VGAT topology.
Histochemical localization of VGAT C terminus
Immunoperoxidase (Fig. 2D–G) and immunofluorescence (supplemental Fig. 2, available at www.jneurosci.org as supplemental material) histochemistry of free-floating vibratome sections processed with or without detergent was performed to confirm the native folding of the two termini of VGAT across SV membranes. Since synapses at the tissue surface are cut open by the vibratome blade antibodies can directly access the surface of SVs (Chaudhry et al., 1998). In the absence of a detergent, anti-VGAT-N reveals punctate terminal-like staining concentrated e.g., in the hippocampal pyramidal cell layer (Fig. 2D) given its free access to the cytosolic VGAT N terminus (Boulland et al., 2008). In contrast, anti-VGAT-C failed to reveal any punctate staining even at high concentrations that otherwise lead to unspecific nuclear staining (Fig. 2E). Terminal-like labeling with anti-VGAT-C when its penetration is facilitated by Triton X-100 bolsters a luminal localization of this epitope (Fig. 2F,G). Immunofluorescence labeling and laser-scanning microscopy yielded identical results (supplemental Fig. 2, available at www.jneurosci.org as supplemental material). Overall, these data demonstrate that the C terminus of VGAT is localized in the lumen of SVs.
Dynamic labeling of GABAergic synapses in vitro
During recycling of SVs, luminal epitopes of SV proteins are temporarily exposed at the cell surface. Proteins recognizing these epitopes can bind to the surface of intact neurons and may become internalized by endocytosis. Indeed, antibodies directed against the N-terminal domain of Syt1 are widely used to selectively label recycling vesicles in nerve terminals (Kraszewski et al., 1995). Therefore, we tested whether anti-VGAT-C can be used to selectively label GABA release sites.
Immunohistochemistry on perfusion-fixed brain sections showed that both unlabeled and Oyster550-labeled anti-VGAT-C selectively recognize GABAergic axon terminals (supplemental Fig. 3, available at www.jneurosci.org as supplemental material). Next, we incubated live neurons for 5 min with anti-VGAT-C (5 μg/ml) with or without Oyster550 label at 37°C. Upon K+-induced depolarization, bright immunofluorescence reminiscent of synaptic puncta were observed (Fig. 3A,B). Selective targeting of unlabeled anti-VGAT-C-derived (Fig. 3A) or Oyster550-anti-VGAT-C (Fig. 3B) to GABAergic synapses was verified by colocalization with anti-VGAT-N. Negligible or no labeling of inhibitory terminals was obtained by applying either cytoplasmic anti-VGAT-N (Fig. 3C) or anti-VGAT-lum1 (data not shown). Application of a CypHer5E-anti-VGAT-C conjugate that only produces fluorescence signal in endocytic vesicles at acidic pH also revealed terminal labeling (supplemental Fig. 4, available at www.jneurosci.org as supplemental material). These data reinforce the luminal localization of VGAT C terminus and its accessibility to antibodies in active synapses.
Labeling of live GABAergic neurons with anti-VGAT-C. A–A″, Uptake of anti-VGAT-C at inhibitory terminals stimulated by 55 mm K+. Incorporated antibodies were visualized after fixation and permeabilization by indirect immunofluorescence. Note prominent colocalization with anti-VGAT-N. B–B″, Detection of inhibitory axon terminals through uptake of Oyster550- anti-VGAT-C of live rat hippocampal neurons in vitro. Anti-VGAT-N was applied indirectly on fixed cells. C–C″, Control showing the lack of anti-VGAT-N uptake (cytoplasmic epitope) by cultured hippocampal neurons; subsequent counterstaining with monoclonal anti-VGAT-N. Yellow/orange color denotes colocalization. Scale bar, 20 μm.
To verify that Oyster550-anti-VGAT-C is selectively taken up by GABAergic synapses, antibody- and mock-treated autaptic cultures of striatal and hippocampal neurons were analyzed by whole cell recordings in voltage clamp mode. Evoked synaptic responses recorded from labeled striatal neurons were exclusively of IPSC type whereas hippocampal neurons staying unlabeled after antibody treatment always exhibited EPSCs. Miniature IPSC amplitudes, their frequency and the readily releasable pool remained unchanged in Oyster550 anti-VGAT-C labeled cells, whereas the evoked IPSC charge was reduced by 46% relative to unlabeled neurons (supplemental Fig. 5, available at www.jneurosci.org as supplemental material). Anti-VGAT-C treated neurons showed a slight, but nonsignificant reduction in vesicular release probability.
In vivo labeling of GABAergic synapses
Our above experiments suggest that anti-VGAT-C may be a versatile tool to visualize GABAergic terminals also in vivo. Therefore, Oyster550-anti-VGAT-C was stereotaxically injected into the CA1 subfield of the hippocampus of adult mice. Bright red immunolabeling reminiscent of the laminar distribution of GABAergic synapses was evident in the injected hemisphere 48 h later (Fig. 4A,B), with labeled structures exhibiting coincident immunoreactivities for VGAT-N (Fig. 4C), GAD (Fig. 4D) and synaptophysin (Fig. 4E). High-resolution images revealed anti-VGAT-C immunoreactivity surrounding (rather than overlapping) GAD-immunopositive structures (Fig. 4D). In vivo labeling and VGLUT1 immunostaining produced distribution patterns complementary with that of indirect VGLUT1 localization (Fig. 4F) underscoring the specificity of anti-VGAT-C immunoreactivity. We conclude that fluorochromated antibodies specific for the C-terminal of VGAT can also be used to label GABAergic terminals in live animals.
In vivo labeling of GABAergic synapses in mouse hippocampus. Direct in vivo VGAT-C labeling in the CA1 region and dentate gyrus (DG) 48 h after intrahippocampal antibody infusion combined with indirect Cy2-immunostaining of parvalbumin, VGAT-N, GAD, synaptophysin, and VGLUT1. A, Distribution of Oyster550-anti-VGAT-C in the injected (left) hippocampal hemisphere. B, Cy3-anti-VGAT-C in the injected hippocampal hemisphere counterstained with antibodies against parvalbumin. C–C″, Oyster550-anti-VGAT-C counterstained with anti-VGAT-N in the CA1 region and combined with anti-GAD in DG (D–D″). Partial coexpression of Oyster550-anti-VGAT-C and synaptophysin in select GABAergic synapses (E–E″). Lack of coexistence of Oyster550-anti-VGAT-C and VGLUT1 in the CA1 region (F–F″). Scale bars: 1 mm (A), 250 μm (B), 10 μm (D), 30 μm (F, also valid for C, E).
Discussion
Here, we show that the C terminus of VGAT localizes to the SV lumen resulting in an uneven number of transmembrane domains. We also demonstrate that antibodies directed against this domain recognize VGAT in intact neurons and are sequestered by endocytosis without major long-term perturbation of synaptic functions, making these immunoreagents versatile tools to identify release sites of GABA and glycine and to study the kinetics of SV recycling in active synapses.
Our knowledge about the topology of VNTs is largely based on computer predictions that often lack experimental confirmation. The TMHMM algorithm predicted a VGAT transmembrane topology which shows major differences compared with previous models (McIntire et al., 1997). In contrast to the model proposed by McIntire et al. (1997), which agrees with most algorithms predicting transmembrane topologies, the AA 243–263 region is classified by the TMHMM algorithm as part of the first cytoplasmic loop rather than a transmembrane domain. Consequently, VGAT exhibits an uneven number of transmembrane domains whose orientation downstream from serine 242 is inversed with the C-terminal 14–16 residues being localized intraluminally instead of being exposed on the outer SV surface. Accordingly, the N and C-terminal and first luminal domains recognized by anti-VGAT-N, anti-VGAT-C and anti-VGAT-lum1, respectively, were differentially sensitive to pronase treatment of isolated SVs. Our proteolytic assays and mass spectrometry do not provide conclusive support for the exact membrane topology of VGAT. Nevertheless, these results unequivocally confirm that the entire N terminus and the loop containing AA 408–415 face the cytoplasm while both epitopes recognized by anti-VGAT-C and anti-VGAT-lum1 are localized in the lumen of SVs.
The major pronase-resistant C-terminal VGAT fragment migrated as ∼10–15 kDa protein. This does not correspond to the expected ∼3–4 kDa size of the fragment comprising the luminal C terminus and the last transmembrane domain which was only visible as a weaker signal. Apparently, the small size of the last putative cytoplasmic loop (5–14 AA, depending on the prediction algorithm) renders it partially resistant to proteolytic cleavage, as also reported for synaptogyrin (Stenius et al., 1995). The larger fragment probably represents the C terminus together with the preceding cytoplasmic and luminal loops and 3 transmembrane domains with a cumulative molecular weight of 10–11 kDa. In contrast, the C termini of VGLUT1, VGLUT2 and VAChT were completely degraded confirming the cytoplasmic localization of these protein epitopes. Thus, VGAT exhibits an exceptional transmembrane topology compared with other known VNTs.
VGAT is the only member of the SLC32 family with no other close mammalian relatives identified so far. Our observations underline the evolutionary distance between VGAT and other known VNTs, which belong to the SLC17 (VGLUT 1–3) or the SLC18 family (VMAT1, 2 and VAChT) (Gasnier, 2004). These latter transporter families are homologous to bacterial toxin/drug extruders (Chaudhry et al., 2008b) while VGAT is not. VGAT even shows significant structural differences when compared with other functionally related membrane located GABA transporters (GAT-1, 2, 3 and BGT-1; (Alexander et al., 2007). The refined VGAT topology with an uneven number of transmembrane domains suggests a closer relationship between VGAT and amino acid permeases in plants which share sequence homology (Wipf et al., 2002) and a similar transmembrane topology with a cytoplasmic localization of the N terminus and a C terminus residing on the outer surface of the plasmalemma (Chang and Bush, 1997). The transmembrane topology for VGAT may also implicate differences in its functional regulation compared with other VNTs whose cytosolic C termini are accessible for other regulatory proteins potentially modulating their function; e.g., endophilin regulation of VGLUT1 recycling (Voglmaier et al., 2006).
Short and transient exposure of live neurons to anti-VGAT-C is sufficient for antibody binding and internalization. Labeling is highly selective for GABAergic neurons indicating that anti-VGAT-C endocytosis is mediated by specific epitope binding and not by unspecific fluid phase uptake. Fluorochromated anti-VGAT-C exhibited a remarkable penetration in vivo. In a subset of synapses, anti-VGAT-C labeling closely surrounded, though did not entirely overlap, GAD immunoreactivity. This may be due to a sterical competition of internalized and perfusion-fixed anti-VGAT-C and subsequently applied anti-GAD. Interestingly, antibody uptake led to a 46% reduction of the overall evoked IPSC amplitude and slightly but not significantly reduced SV release probability. The individual miniature fusion events, their frequency and the readily releasable vesicle pool remained unchanged indicating that the presence of luminal anti-VGAT-C can lead to subsequent reduction of the efficiency of SV release but does not affect the translocation of GABA into SVs.
Understanding the precise spatial localization of the VGAT C terminus to the SV lumen is vital for exploiting the potential of our approach to live cell imaging and physiology models of inhibitory neurotransmission. Our confocal imaging studies present the possibility of using VGAT as a novel biomarker in studies aimed at elucidating organizing principles of inhibitory synapses. Combined use of in vivo labeling and tracer ligands holds promise for revealing novel synaptic circuitries and refined innervation patterns in the brain. Moreover, in vivo labeling of functionally intact and electrophysiologically active GABAergic terminals will yield a new dimension of understanding key features of inhibitory neurotransmission, and their perturbations in neuropathological conditions.
Footnotes
This work was supported by the Bioprofile Program (BMBF; #0313132), EU Synapse (LSHM-CT-2005-019055), the Interdisciplinary Center of Clinical Research at the Faculty of Medicine of the Universität Leipzig (Z10), the Alzheimer's Association, and the Swedish Medical Research Council. We gratefully thank Matthias Böddener and Ute Bauer for technical help and Sonja Wojcik (Max Planck Institute for Experimental Medicine, Göttingen, Germany) for providing VGAT knock-out tissue samples.
- Correspondence should be addressed to Dr. Henrik Martens, Synaptic Systems GmbH, Rudolf Wissell Strasse 28, D-37079 Göttingen, Germany. lab{at}sysy.com
This article is freely available online through the J Neurosci Open Choice option.
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