Figure 6.
NgR1 ligand-binding domain variants interact with known coreceptors but do not mediate myelin signaling. A, Interaction of NgR1 Italian variants with putative NgR1 coreceptors. HEK293T cells were transfected with the indicated expression vectors (WT-NgR, WT; R119W-NgR, W; R196H-NgR, H). Lysates were immunoblotted directly or subjected to immunoprecipitation and then immunoblot, as indicated. These NgR1 amino acid variations do not significantly alter interaction with p75 or Lingo-1. B, Growth cone collapse induced by myelin ligands in E7 retinal explants expressing WT-NgR1, R119W-NgR1, or R196H-NgR1. Values with variant NgR1-expressing virus are significantly different from those with wild-type NgR1 expression (*p < 0.05, one-way ANOVA). C, Dominant-negative effect of NgR1 variants in E13 cDRG expressing indicated constructs. Values with variant NgR1-expressing virus are significantly different from those with wild-type NgR1 expression (*p < 0.05, one-way ANOVA). All data are mean ± SEM from n = 3–6 experiments.
