Figure 7.
L655,703 antagonizes GABAA receptor-mediated tonic currents in D2+ MSNs. A, Representative trace showing the effects of L655,703 (10 μm) on sIPSCs and on BIC-sensitive tonic current in a D2+ MSN. Calibration: 20 ms, 30 pA. B, Summary of tonic current blocked by L655,703 (50 nm, n = 4; 10 μm, n = 8) in D2+ MSNs. C, Representative traces showing the effects of L655,703 (10 μm) on sIPSCs and BIC-sensitive tonic currents elicited by bath application of 1 μm GABA in a D1+ MSN and a D2+ MSN. Calibration: 20 ms, 30 pA. D, Summary of the percentage blockade by L655,708 of the BIC-sensitive tonic current elicited by bath application of 1 μm GABA in D1+ MSNs (100 nm, n = 3; 10 μm, n = 5) and in D2+ MSNs (50 nm, n = 2; 10 μm, n = 4). E, Representative traces showing overlapping sIPSCs recorded in a D1+ MSN (top) and a D2+ MSN (middle) in the presence (gray trace) and the absence (black trace) of 10 μm L655,703, as well as the summary of the changes in sIPSC amplitude (Amp) and decay time (Tau) produced by 10 μm L655,703 in six D1+ and seven D2+ MSNs (bottom) represented as percentage of baseline values. Calibration: 20 ms, 30 pA.