Article Figures & Data
Figures
- Figure 1.
Hemicerebellectomy induces CB2R expression in precerebellar stations. A, Confocal images from the Pn of CTRL-Eth and HCb-Eth animals, reacted with CB1R and CB2R antibodies and counterstained with DAPI. B, Number of Nissl-stained and CB2R-positive neurons in the IO and Pn in CTRL-Eth and HCb-Eth rats. Data are reported as means ± SD (n = 5 animals per group). C, Confocal images showing CB2R and NeuN immunostaining plus DAPI counterstaining. D, CB1R, CB2R, and enolase mRNA expression in the IO and Pn of HCb-Eth rats. Data are presented as fold changes in target gene expression, normalized to the internal control gene and relative to the tissue control. Data are reported as means ± SD (n = 4; each performed in triplicate). ***p < 0.0001. Scale bars: A, 40 μm; C, 10 μm.
- Figure 2.
Effects of treatment with selective CB1R, CB2R, and TRPV1 agonists or antagonists on neuronal survival (A) and NSS (B–D). Data are reported as means ± SD (n = 5 animals per group). ***p < 0.0001.
- Figure 3.
JWH-015 treatment modulates cytochrome-c (cyt-c) release and p-Akt and p-JNK expression in HCb animals. A, cyt-c and NeuN double-labeling confocal images from Pn of CTRL-Eth, HCb-Eth, HCb-SR2, and HCb-JWH animals. B, Percentages of NeuN-positive neurons releasing cyt-c in the IO and Pn in CTRL-Eth, HCb-Eth, HCb-JWH, and HCb-SR2 animals. C, Western blot analysis of Akt, p-Akt, JNK, and p-JNK proteins. Band levels (left) and densitometric values (right) are shown. ***HCb-JWH versus HCb-HCb-Eth and HCb-JWH versus HCb-SR2 (p-Aht values); *HCb-JWH versus HCb-HCb-Eth and HCb-JWH versus HCb-SR2 (p-JNK values). D, p-Akt and NeuN double-labeling confocal images from Pn of CTRL-Eth, HCb-Eth, HCb-JWH, and HCb-SR2 rats. E, Time course of densitometric values of p-Akt expressed as mean fluorescence of individual cells normalized to total cellular surface (F/A) in CTRL-Eth, CTRL-JWH, CTRL-SR2, HCb-Eth, HCb-JWH, HCb-SR2, HCb-LY-JWH, and HCb-SP-JWH rats. F, Confocal images from Pn of CTRL-Eth, HCb-Eth, HCb-JWH, and HCb-SR2 rats reacted with p-JNK antibody and counterstained with DAPI. G, Time course of densitometric values of p-JNK expressed as mean fluorescence of individual cells normalized to total cellular surface (F/A) in CTRL-Eth, CTRL-JWH, CTRL-SR2, HCb-Eth, HCb-JWH, HCb-SR2, HCb-LY-JWH, and HCb-SP-JWH rats. B, C, E, G, Data are reported as means ±SD (n = 5 animals per group). *p < 0.05; ***p < 0.001. Scale bars: 8 μm.
- Figure 4.
The PI3K inhibitor LY294002 abolishes and JNK inhibitor SP600125 does not affect JWH-015-induced neuroprotective effects. Number of surviving neurons (A), percentages of neurons releasing cyt-c (B), and NSS values (C) in HCb-Eth, HCb-JWH, HCb-LY-Eth, HCb-LY-JWH, HCb-SP-Eth, and HCb-SP-JWH animals. Data are reported as means ± SD (n = 5 animals per group).
Tables
Group codes n HCb Drug treatment CTRL-Eth 16 Ethanol (400 μl, 0.5% saline, i.p., for 7 d) HCb-Eth 16 X Ethanol (400 μl, 0.5% saline, i.p., for 7 d) CTRL-ACEA 5 ACEA (5 mg/kg, i.p., for 7 d) HCb-ACEA 5 X ACEA (5 mg/kg, i.p., for 7 d) CTRL-SR1 5 SR141716A (3 mg/kg, i.p., for 7 d) HCb-SR1 5 X SR141716A (3 mg/kg, i.p., for 7 d) CTRL-JWH 16 JWH-015 (3 mg/kg, i.p., for 7 d) HCb-JWH 16 X JWH-015 (3 mg/kg, i.p., for 7 d) CTRL-SR2 16 SR144528 (3 mg/kg, i.p., for 7 d) HCb-SR2 16 X SR144528 (3 mg/kg, i.p., for 7 d) CTRL-CAPS 5 Capsaicin (1 mg/kg, i.p., for 7 d) HCb-CAPS 5 X Capsaicin (1 mg/kg, i.p., for 7 d) CTRL-CAPZ 5 Capsazepine (3 mg/kg, i.p., for 7 d) HCb-CAPZ 5 X Capsazepine (3 mg/kg, i.p., for 7 d) CTRL-LY-Eth 5 LY294002 (15 μg/10 μl; 25% DMSO, i.c.v.) + ethanol (400 μl, 0.5% saline, i.p., for 7 d) HCb-LY-Eth 5 X LY294002 (15 μg/10 μl; 25% DMSO i.c.v.) + ethanol (400 μl, 0.5% saline, i.p., for 7 d) CTRL-LY-JWH 5 LY294002 (15 μg/10 μl; 25% DMSO i.c.v.) + JWH-015 (3 mg/kg, i.p., for 7 d) HCb-LY-JWH 5 X LY294002 (15 μg/10 μl; 25% DMSO i.c.v.) + JWH-015 (3 mg/kg, i.p., for 7 d) CTRL-SP-Eth 5 SP600125 (30 μg/10 μl; 25% DMSO i.c.v.) + ethanol (400 μl, 0.5% saline, i.p., for 7 d) HCb-SP-Eth 5 X SP600125 (30 μg/10 μl; 25% DMSO i.c.v.) + ethanol (400 μl, 0.5% saline, i.p., for 7 d) CTRL-SP-JWH 5 SP600125 (30 μg/10 μl; 25% DMSO i.c.v.) + JWH-015 (3 mg/kg, i.p., for 7 d) HCb-SP-JWH 5 X SP600125 (30 μg/10 μl; 25% DMSO i.c.v.) + JWH-015 (3 mg/kg, i.p., for 7 d) -
CTRL, Control.
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Supplemental Data
Files in this Data Supplement:
- supplemental material - Supplemental Material
- supplemental material - Supplemental Legend
- supplemental material - Supplemental Table
- supplemental material - Supplemental Figure 1
- supplemental material - Supplemental Figure 2
- supplemental material - Supplemental Figure 3
