Figure 4.
Requirement of protein synthesis in CA1 but not CA3 neurons in hippocampal L-LTP. GFP or FKBP-PKR-GFP Sindbis virus was injected into either CA1 or CA3 region of hippocampus of 8-week-old C57BL/6 mice. Approximately 36–48 h after in vivo injection, the animals were killed and hippocampal slices were prepared. A, Localization of FKBP-PKR, as indicated by GFP fluorescence, in the CA1 area. The arrowhead indicates the viral injection site. B, Normal E-LTP in FKBP-PKR injected slices treated with vehicle or AP20187. Sample traces before (“1”) and 3 h (60 min for B) after (“2”) TBS stimulation in this and other figures are shown on top of each figure. C, Inhibition of L-LTP by AP20187 in slices expressing FKBP-PKR in CA1. L-LTP was induced by 12-TBS. The difference between vehicle- (155 ± 8%, SEM) and AP20187- (104 ± 3%) treated slices at “2” is highly significant (p < 0.0002, t test). D, Normal L-LTP in AP20187-treated slices expressing FKBP-PKR in CA3.