Article Figures & Data
Figures
- Figure 1.
Analysis of Nogo-A,B gene-trap mutant mice for CST regeneration and locomotor recovery. A , B , Representative sagittal spinal cord sections showing the labeled main CST tract approaching but not passing through the injury site (arrow). Rostral is to the left. Het, Heterozygous; KO, knock-out (mutant). Scale bar, 500 μm. C , Quantification of CST axons along the rostrocaudal axis on sagittal sections did not reveal any significant differences between the two genotypes. D , Locomotor recovery as assessed by a modified BBB open-field score did not show any significant differences between the two genotypes.
- Figure 2.
Generation and molecular characterization of the Nogo deletion mutant. A , The strategy to obtain the Nogo deletion allele via two consecutive gene targeting steps followed by Cre-mediated excision. A, B, C above the exons (black bars) refer to Nogo isoforms. Dotted lines, Splicing pattern for the Nogo-C transcript; Neo, neomycin resistance gene; Hprt, hypoxanthine phosphoribosyltransferase gene; IRES, internal ribosomal entry site; GFPLacZ, green fluorescent protein and β-galactosidase fusion gene; small arrows, primers used to analyze the Nogo-C transcript by RT-PCR. Two Frt sites flanking Hprt are omitted for simplicity. B , Northern blot with a C-terminal common probe. Nogo-A,B,C transcripts run at 4.7, 2.6, and 1.7 kb, respectively. Arrowheads indicate a slightly shorter, mutated form of Nogo-C transcript. WT, Wild type. C , RT-PCR to confirm the absence of Nogo-C transcript in the Nogo deletion mutant. D , Western blot analysis on Nogo-A, NgR1, MAG, and OMgp in Nogo-A,B gene-trap and Nogo deletion mutants. α-tub, α-Tubulin.
- Figure 3.
Analysis of Nogo deletion mutant mice for CST regeneration. A , B , Representative sagittal spinal cord sections showing the labeled main CST tract approaching but not passing through the injury site (arrow). ND, Nogo deletion; WT, wild type; KO, knock-out. Rostral is to the left. Scale bar, 500 μm. C , Quantification of CST axons caudal to the injury site did not indicate any significant differences between the two genotypes, but wild-type mice showed significantly more axons at two distance points rostral to the injury site. *p < 0.05 two-way repeated measures ANOVA with Bonferroni's post hoc test.
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