Figure 5.
Spike RF structures of identified excitatory and inhibitory neurons in layer 2/3. A, B, Spike RFs of an example GFP(+) inhibitory neuron (A) and a GFP(−) excitatory neuron (B). Plotting is similar to that in Figure 1A. Left, Traces of example spike waveforms. Calibration: horizontal, 1 ms; vertical, 100 pA (A) and 60 pA (B). Middle, PSTH for On and Off responses to all the stimuli. Red and blue ovals depict the Gaussian fits of the On and Off subfields, respectively. Each pixel represents 5° in visual space. Calibration: horizontal, 200 ms; vertical, 36 Hz (A) and 16 Hz (B). Right, Superimposed color maps for On (red) and Off (green) responses. C, Outlines of Gaussian fits of spike On and Off subfields for the three types of neurons. Red and blue ovals represent On and Off subfields, respectively. RFs were binned according to their OI values. Bin size, 0.2. Scale bar, 10°. D, Distribution of OI (triangle) and correlation coefficient (circle) for different groups of neurons. n = 18, 17, and 17 for GFP(+) FS, GFP(+) RS, and GFP(−) RS neurons, respectively. *p < 0.001, Tamhane T2 multiple comparison test. E, Distribution of subfield size. For S-RF and O-RF cells, subfield size was the average of On and Off subregions. n = 21, 20, and 20 for GFP(+) FS, GFP(+) RS, and GFP(−) RS neurons, respectively. *p < 0.001. F, Distribution of modulation ratio. n = 25 and 24 for GFP(+) and GFP(−) neurons, respectively. *p < 0.001, t test. Solid symbols indicate mean value, and error bars indicate SD.