Figure 4.
NTE deficiency induces increased neural PtdCho levels and impairs neuronal secretion. A, B, PtdCho and cholesterol levels in brain cortex of vehicle-dosed (10 mice) and MOCDPP-dosed (4 per time point) adult C57BL/6J mice (A), and WT and NTE–cKO mice (1–3 months, 6 mice; 6–12 months, 12 mice per group, respectively) (B). Data are mean ± SEM. *Significantly different (p < 0.05; Student's t test) from vehicle-dosed control value (A) or respective WT mean value (B). C–F, Secretion from cultured CGN of reelin and APP. C, D, Top, Representative Western blots of paired cell lysates and conditioned media harvested from cultures of WT CGN and probed for reelin (C) or APP (D). Bottom, Data from multiple blots quantified to show secretion index (the ratio of extracellular to intracellular reelin or APP) as a function of incubation time. Data are means ± SEM of 4 (C) or 6–8 (D) individual culture wells from each of three separate WT CGN preparations. E, F, Representative Western blots of paired cell lysates and conditioned media harvested at 2 h from WT and littermate NTE–cKO CGN and probed for reelin (E) or APP (F).