The Parkinson Disease Protein Leucine-Rich Repeat Kinase 2 Transduces Death Signals via Fas-Associated Protein with Death Domain and Caspase-8 in a Cellular Model of Neurodegeneration

This work was supported by National Institutes of Neurological Disorders and Stroke Grants K02 NS045798 and R01 NS061098, the Parkinson Disease Foundation, and the Anne and Bernard Spitzer Center for Cell and Genetic Therapy for Parkinson Disease. We thank the following people for their helpful comments on this manuscript: Serge Przedborski, Jonathan Javitch, Gilbert DiPaolo, Chris Henderson, and members of the Dauer Laboratory. Thanks also to Kana Tsukamoto for outstanding technical assistance. We also thank Drs. Dennis Dickson and Zbigniew Wszolek of the Mayo Clinic (Jacksonville, FL) for providing LRRK2-positive human brain tissue, Dr. Jean-Paul Vonsattel of the New York Brain Bank for providing control and idiopathic PD brain tissue, and Dr. Lorraine Clarke of Columbia University for genotyping idiopathic PD samples. The plasmid for caspase-8 was a kind gift from Dr. Junying Yuan; the plasmid for LZ-FADD-DD was a kind gift from Dr. Milton Werner; the plasmid for death receptor 3 was a kind gift from Dr. Shie-Liang Hsieh; the LRRK2 antibody was a kind gift from Dr. Zhenyu Yue; the anti-mouse FADD antibody was a kind gift from Dr. Andreas Strasser.