Figure 3.
Histological stainings of wild-type (A, C, E, G) and erbb2/erbb4-deficient (B, D, F, H) cerebellum. Scale bar, 50 μm. A, B, Cresyl violet staining of wild-type (A) and erbb2/erbb4-deficient (B) cerebellum. Purkinje cells formed a continuous monolayer, and the granule cell and molecular layer were of normal thickness. C, D, Double immunofluorescence for calbindin (red) to label Purkinje cells and for NeuN (green) to label granule cells. No difference can be seen between control (C) and erbb2/erbb4-deficient (D) cerebellum. E, F, Staining for GFAP revealed an arrangement of Bergmann glia cell processes in the molecular layer, which is not distinguishable in both genotypes. Bright staining on top of the molecular layer in (E) represents staining in the meninges (removed in F). G, H, Staining for vGlut2 to reveal synaptic glomeruli in the granule cell layer and climbing fibers in the molecular layer. No difference was detected between wild-type (G) and erbb2/erbb4-deficient (H) cerebellum. Bright staining on top of the molecular layer in (E) represents staining in the meninges (removed in F).