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Glutamate decarboxylase immunoreactivity and gamma-[3H] aminobutyric acid accumulation within the same neurons in dissociated cell cultures of cerebral cortex

EA Neale, WH Oertel, LM Bowers and VK Weise
Journal of Neuroscience 1 February 1983, 3 (2) 376-382; DOI: https://doi.org/10.1523/JNEUROSCI.03-02-00376.1983
EA Neale
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WH Oertel
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LM Bowers
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VK Weise
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Abstract

In order to evaluate the reliability of high affinity [3H]GABA accumulation as a marker for GABAergic neurons, murine cerebral cortical neurons were studied in dissociated cell culture. Cultures which had been incubated in [3H]GABA were stained immunohistochemically for the GABA-synthesizing enzyme, glutamate decarboxylase, fixed with paraformaldehyde, and subsequently processed for radioautography. In mature cultures, there was an 84 to 94% correlation between the presence of the enzyme and [3H]GABA uptake within the same cortical neurons. These data provide direct evidence that those neurons which synthesize GABA are the same neurons which are labeled by high affinity [3H]GABA uptake.

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The Journal of Neuroscience: 3 (2)
Journal of Neuroscience
Vol. 3, Issue 2
1 Feb 1983
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Glutamate decarboxylase immunoreactivity and gamma-[3H] aminobutyric acid accumulation within the same neurons in dissociated cell cultures of cerebral cortex
EA Neale, WH Oertel, LM Bowers, VK Weise
Journal of Neuroscience 1 February 1983, 3 (2) 376-382; DOI: 10.1523/JNEUROSCI.03-02-00376.1983

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Glutamate decarboxylase immunoreactivity and gamma-[3H] aminobutyric acid accumulation within the same neurons in dissociated cell cultures of cerebral cortex
EA Neale, WH Oertel, LM Bowers, VK Weise
Journal of Neuroscience 1 February 1983, 3 (2) 376-382; DOI: 10.1523/JNEUROSCI.03-02-00376.1983
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