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Brief Communications

The Functional Properties of Barrel Cortex Neurons Projecting to the Primary Motor Cortex

Takashi R. Sato and Karel Svoboda
Journal of Neuroscience 24 March 2010, 30 (12) 4256-4260; DOI: https://doi.org/10.1523/JNEUROSCI.3774-09.2010
Takashi R. Sato
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Karel Svoboda
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Abstract

Nearby neurons, sharing the same locations within the mouse whisker map, can have dramatically distinct response properties. To understand the significance of this diversity, we studied the relationship between the responses of individual neurons and their projection targets in the mouse barrel cortex. Neurons projecting to primary motor cortex (MI) or secondary somatosensory area (SII) were labeled with red fluorescent protein (RFP) using retrograde viral infection. We used in vivo two-photon Ca2+ imaging to map the responses of RFP-positive and neighboring L2/3 neurons to whisker deflections. Neurons projecting to MI displayed larger receptive fields compared with other neurons, including those projecting to SII. Our findings support the view that intermingled neurons in primary sensory areas send specific stimulus features to different parts of the brain.

Introduction

In the mouse barrel cortex (Woolsey and van der Loos, 1970), the locations of the neurons within the somatotopic map account for only a small part of the response heterogeneity; even neighboring neurons sharing the same barrel column can have very different whisker selectivities (Sato et al., 2007). Does this heterogeneity reflect highly specific subcolumnar circuits (Song et al., 2005; Yoshimura et al., 2005) or simply noise in neocortical wiring? The heterogeneity of neural responses could be related to the diverse cell types intermingled within the cortex, such as excitatory and inhibitory cells (Simons, 1978; Brumberg et al., 1999; Sohya et al., 2007). Neurons with specific projection targets can also have distinct response properties (Evarts, 1965; Hikosaka and Wurtz, 1983; Swadlow, 1988, 1989; Movshon and Newsome, 1996; Ferraina et al., 2002; Briggs and Usrey, 2009). Projection-dependent neuronal function has been probed with extracellular unit recordings combined with antidromic activation (Evarts, 1965; Movshon and Newsome, 1996; Ferraina et al., 2002), but this method is inefficient, even between areas with strong connections [e.g., V1 and MT in the macaque (Movshon and Newsome, 1996)]. In addition, this method does not provide information on the precise locations of the antidromically stimulated neurons, and projection-dependent functional differences could thus still be related to cell location (e.g., cortical layer or minicolumn) (Ferraina et al., 2002) rather than the projection pattern per se.

To overcome these limitations, we combined bulk loading of a green Ca indicator (Stosiek et al., 2003) and retrograde labeling with a virus [herpes simplex virus 1 (HSV1)] (Lilley et al., 2001) expressing a red fluorescent protein (RFP; DsRed-Express) (see Fig. 1A,B). This method allows us to simultaneously image the responses of retrogradely labeled (RFP+) and nearby nonlabeled (RFP−) neurons. We found that neurons that project to the primary motor cortex (MI) have larger receptive fields compared with other neurons, including those that project to secondary somatosensory area (SII).

Materials and Methods

Virus preparation.

cDNA of DsRed-Express was subcloned between EcoRI and BsrGI in the HSV1 shuttle vector obtained from Biovex. Expression of DsRed-Express was driven by the EF1α promoter. A disabled version of HSV1 was produced by Biovex (Lilley et al., 2001; Lima et al., 2009).

Virus injection.

All experimental protocols were conducted according to the National Institutes of Health guidelines for animal research and were approved by the Institutional Animal Care and Use Committee at Janelia Farm Research Campus. C57BL/6J mice (Charles River) were deeply anesthetized using an isoflurane–oxygen mixture (1% vol isoflurane/vol O2) at postnatal day 12–14 and placed in a custom stereotactic apparatus. A small incision was made in the skin to identify the location of bregma, and then was immediately closed and sutured. Then, another small incision was made and small holes were drilled in the scalp at the injection site, either MI (1.2 mm anterior to bregma, 0.6 mm lateral) or SII (0.7 mm posterior to bregma, 4.2 mm lateral). Microstimulation in MI resulted in whisker movements. Fifty nanoliters of viral suspension (titer, 2 × 109 pfu/ml) was injected (over 1 min) using a pulled glass micropipette (tip diameter, 10–20 μm; Drummond). To prevent backflow, the micropipette was left in the brain for 10 min before pulling up. The scalp was closed and sutured, and then the animals were allowed to recover on a heated pad. RFP expression took place for 7–14 d before the imaging experiments. Similar procedures were used for bead injections (undiluted stock, LumaFluor).

Animals and surgical procedures.

Mice (postnatal day 20–27) were anesthetized using an isoflurane–oxygen mixture (1% vol isoflurane/vol O2). Core body temperature was maintained at 37°C using a heating blanket (Harvard Apparatus). Imaging windows were installed above the barrel cortex. A small craniotomy (diameter, 1–2 mm) was made 1 mm posterior from bregma and 3.5 mm lateral from the midline on the right hemisphere. The intact dura was covered with 2% agarose (Type-IIIA, Sigma), dissolved in HEPES-buffered artificial CSF, and a 5 mm cover glass (World Precision Instruments). The cover glass was sealed in place using dental acrylic, leaving one side open for electrode access (Svoboda et al., 1997, 1999; Sato et al., 2007).

Loading procedures.

Following the craniotomy, the mice were placed under a custom-made two-photon microscope and anesthetized with ketamine (160 mg/kg) and xylazine (10 mg/kg). Layer 2/3 neurons in the barrel cortex (rows D, C, B; arcs 1, 2, 3, 4, 5) were loaded with Fluo-4 AM (F14201, Invitrogen) using multi-cell bolus loading (Stosiek et al., 2003; Ohki et al., 2005; Sato et al., 2007). The choice of Fluo-4 AM, compared with other calcium indicators (e.g., Oregon Green Bapta-1 AM), was based on its high signal-to-noise ratio (Sato et al., 2007). Fluo-4 AM was dissolved in 20% (w/v) Pluronic F-127 (P-6867, Invitrogen) in DMSO to a concentration of 10 mm. This solution was then diluted 10-fold into external buffer containing (in mm): 125 NaCl, 5 KCl, 10 glucose, 10 HEPES, 2 CaCl2, and 2 MgSO4. In some experiments, 1–100 nm Alexa was added to visualize the flow. Glass micropipettes (tip resistance, ∼1 MΩ) were filled with the loading solution and inserted into the barrel cortex. The tips of the micropipettes were brought into a cortical region expressing DsRed-Express. Repetitive pulses of positive pressure (5–10 psi, 10 ms, 10–20 times; PicoSpritzer II, General Valve) were applied to eject the dye to bulk load a small tissue volume (diameter, ∼200 μm).

Two-photon microscopy.

In vivo imaging was performed using a custom-made two-photon laser-scanning microscope controlled by ScanImage software (Pologruto et al., 2003). The light source for Fluo-4 imaging was a pulsed Ti:sapphire laser (λ, ∼810 nm; 50–150 mW in the objective back-focal plane; MaiTai, Spectra-Physics) and for DsRed-Express imaging was a solid state Ytterbium laser (λ, ∼1030 nm; 100–200 mW in the objective back-focal plane; t-Pulse, Amplitude Systemes). Red and green fluorescence photons were separated using a 565 nm dichroic mirror (Chroma Technology) and barrier filters (green, BG22; red, 607/45; Chroma Technology). Signals were collected using photomultiplier tubes (3896 or H7422P-40 MOD, Hamamatsu). The objective lens (40, 0.8 NA) and trinoc were from Olympus. We used frame scanning (frame rate, 15.6 Hz) with 2 ms line durations (32 × 1024 pixels). Pixel dimensions were 0.10 × 1.3 μm. Images were collected in layer 2 and 3, 120–250 μm from the top of the dura (Woolsey and van der Loos, 1970; Bureau et al., 2006; Sato et al., 2007).

Sensory stimulation.

The loading pipette was used to measure the sensory stimulation-evoked local field potential (LFP). A hand-held stimulator was used to identify whiskers that were effective in evoking LFPs in the area loaded with Ca2+ indicator. These whiskers were then deflected using a piezoelectric stimulator (300 μm deflection in the rostral–caudal direction, positioned 5 mm from the base of the whisker) while recording the LFP. The pair of whiskers evoking the largest LFP response [principal whisker (PW)] and the second largest LFP response [surround whisker (SW)] were used for further mapping.

Imaging sessions began 30 min after dye loading. Trial durations were 960 ms (15 frames). To minimize use-dependent depression, intertrial intervals were long (20–30 s) (Armstrong-James, 1975) (supplemental Fig. 2, available at www.jneurosci.org as supplemental material). Whiskers were stimulated in an interleaved manner. The imaging session lasted 1–1.5 h (100–200 trials). Two factors conspired against longer imaging sessions. First, Fluo-4AM was extruded from the neuronal cytoplasm. Second, extracellular baseline fluorescence increased with time due to unknown causes (Koester et al., 1999).

In some experiments, after the imaging sessions fluorescent beads were injected into the center of the imaging site, and the PW was verified anatomically by examining the barrel pattern in tangential sections (100 μm thick) through layer 4 stained with cytochrome oxidase (Wong-Riley and Welt, 1980; Land and Simons, 1985).

Data analysis.

Single whisker deflections cause zero, one, or, rarely, two action potentials in L2/3 neurons (Simons, 1978; Armstrong-James and Fox, 1987; Svoboda et al., 1997; Brecht and Sakmann, 2002; Brecht et al., 2003; Sato et al., 2007). Action potentials cause Ca2+ accumulations in somata and dendrites that can be detected with Ca2+ imaging in vivo (Svoboda et al., 1997, 1999; Waters and Helmchen, 2004). Because individual dendrites were difficult to identify, we performed fluorescence measurements in somata. Regions of interest were manually selected inside the somata.

Trials that resulted in action potential-evoked fluorescence changes and trials that did not produce fluorescence changes were separated using two parameters, as described previously (Sato et al., 2007): (1) The change in ΔF/F between the last prestimulus frame and the first poststimulus frame. Because spikes can occur up to ∼50 ms after whisker deflection (Glazewski et al., 1996; Brecht et al., 2003), we also computed the difference between the first and second poststimulus frames. Fd was defined as the larger of these values. (2) The amplitude, AF, of the fluorescence transient derived from template matching (Clements and Bekkers, 1997). The template was: Embedded Image Embedded Image The time constant of the template, τ, was the decay time of the exponential fluorescence transients (1 s). The origin (0 ms) of the template window (−192 to 256 ms, eight frames) was positioned at the frame of interest, and amplitude and offset were adjusted to fit the data by minimizing the sum of squared errors.

We plotted Fd and AF for all trials (see Fig. 2B) and then applied hierarchical clustering, using the Euclidean distance, to define the distance between points. For many cells, the trials fell into two groups, corresponding to failures and successes. To quantify the separation between these groups, we calculated the 95% confidence ellipse for each of the two clusters. We focused our analysis on those cells in which the two confidence ellipses were separated, and discarded the rest. Assigning responsive trials and nonresponsive trials in this manner resulted in error rates of <1% (Sato et al., 2007).

For all neurons in which more than five responsive trials were detected, the response probability (Pr) was determined for each cell and whisker (PW, SW) as the number of responsive trials divided by the total number of trials. The ratio of these two values was calculated as follows: Embedded Image

Results

Following injection of viral suspension into MI, RFP+ neurons were found in the contralateral primary motor cortex, ipsilateral SII, ipsilateral posterior cingulate cortex (29c), ipsilateral thalamus, and layers 2/3 and 5 of the ipsilateral barrel cortex. This pattern was largely consistent with previous studies using HRP injections (Porter and White, 1983) and our own data obtained with fluorescent beads, a classic retrograde tracer (Katz et al., 1984) (Fig. 1C,D). The labeling efficiency with HSV1 was lower compared with beads (∼30%; comparing areas with the densest labels) (Fig. 1C, compare left and right). However, HSV1 infection provided brighter labeling, allowing us to image RFP+ cells in vivo (Fig. 1B). In contrast, bead-labeled neurons were harder to detect in vivo.

Figure 1.
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Figure 1.

Retrograde labeling with a virus expressing a red fluorescent protein. A, Schematic showing retrograde labeling of neurons in SI by injection of the retrograde virus HSV1 into MI. B, In vivo image of RFP+ neurons (maximum-intensity side projection of an image stack of RFP+ neurons; 512 × 128 × 96; section spacing, 8 μm). C, Distribution of labeled neurons in SI barrel cortex after bead (left) or virus (right) injection into MI. The white lines indicate the pia and the border between the cortex and the white matter. D, Normalized distribution of labeled neurons after bead (black, 1293 neurons) and HSV (white, 808 neurons) injection into MI.

We loaded a cluster (diameter, ∼200 μm) of layer 2/3 neurons, including RFP+ neurons, in the barrel cortex with the Ca indicator Fluo-4 AM (see Materials and Methods) (Fig. 2A) (Stosiek et al., 2003; Ohki et al., 2005; Sato et al., 2007). In the green fluorescence channel, loaded neurons could be identified as areas of relatively homogenous, dim green fluorescence against a highly heterogeneous fluorescent background (Fig. 2A, left). In the red channel, RFP+ cells showed bright red fluorescence on a dark background (Fig. 2A, right, cell 1). We used field potential measurements to determine the PW and the SW producing the largest responses, at the center of the imaging window (supplemental Fig. 1A, available at www.jneurosci.org as supplemental material) (Sato et al., 2007).

Figure 2.
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Figure 2.

The receptive fields of barrel cortex neurons with projections to MI or SII. A, Left, Cells were loaded with Fluo-4 AM; right, one cell in the field of view (RFP+, cell 1) sends axons to MI. B, Examples of whisker stimulation-evoked fluorescence transients for three cells on two different trials. Columns correspond to the three cells identified in A. Red traces indicate trials with action potential-evoked fluorescence transients, blue traces indicate trials without fluorescence transients. Whisker stimuli are indicated at the bottom of C. C, Multiple fluorescence transients from cells 1–3 aligned on the whisker stimulus (bottom). D, Plots of amplitude (AF) and difference (Fd) (see supplemental Methods, available at www.jneurosci.org as supplemental material) for all trials for the cells identified in A. The points were clustered into two groups (red, successes; blue, failures). The 95% confidence ellipses are overlaid on the graph. E, The response probability of these three cells to stimulation of D4 and D5. F, The distribution of the ratios of response probabilities to PW and SW stimulation following the virus injection into MI (left) and SII (right). The red bars indicate data from RFP+ cells and the white bars indicate the data from RFP− cells.

We next mapped the receptive fields of Fluo-4 AM-labeled neurons by stimulating the PW and SW. Following a whisker deflection, most neurons exhibited fluorescence changes in some trials but not in other trials. In many neurons, trials with and without fluorescence changes could be reliably distinguished (e.g., cells 1–3) (Fig. 2B–D), and we focused our analysis on these neurons (256/351 cells); the remainder of the neurons, for which “spike sorting” was not possible, were not analyzed further. Trials with fluorescence changes correspond to trials with single action potentials, whereas trials without fluorescent changes correspond to trials without action potentials (Sato et al., 2007).

For example, the RFP+ cell 1 responded equally often to stimulation of PW and SW: the response probability was 0.36 for PW stimulation (149 trials), and 0.35 for SW stimulation (150 trials) (Fig. 2E). However, the neighboring RFP− neurons (cells 2, 3) were more selective to PW stimulation (cell 1: PW, 0.17; SW, 0.05; cell 2: PW, 0.13; SW, 0.01). Thus, in this experiment the RFP+ cell had a larger receptive field and a higher response probability compared with the neighboring neurons.

We quantified the receptive field size by calculating the ratio of the response probability to PW and SW stimulation. When neurons were labeled by injecting HSV1 into MI, the ratio was larger for RFP+ neurons compared with RFP− neurons (RFP+ neurons, n = 40, 0.71 ± 0.26; RFP−, n = 117, 0.52 ± 0.28; mean ± SD; p < 0.00001, for comparison between the actual data and the data shuffled within each imaging session) (Fig. 2F, left), implying that MI-projecting neurons have larger receptive fields. In addition, the response probability for the PW was larger for RFP+ neurons compared with RFP− neurons (RFP+ neurons, n = 40, 0.61 ± 0.24; RFP− neurons, n = 120, 0.55 ± 0.27; mean ± SD; p < 0.05, for comparison between the actual data and the data shuffled within each imaging session).

We next examined the functional properties of neurons projecting to SII (supplemental Fig. 1B, available at www.jneurosci.org as supplemental material). Using double injection of red and green fluorescent beads into MI and SII, we confirmed that only a small portion of neurons project to both MI and SII: only 40/993 cells were double labeled (394 cells labeled from MI, 639 cells from SII from four mice; analysis was focused on areas where the labeling was most dense) (supplemental Fig. 1C, available at www.jneurosci.org as supplemental material) (Chakrabarti and Alloway, 2006). Following HSV1 injection into SII, we measured the response properties of RFP+ neurons in the barrel cortex (Fig. 2F, right). The ratio of the response probabilities to PW and SW stimulation were similar between RFP+ and RFP− neurons (RFP+ neurons, n = 24, 0.57 ± 0.31; RFP− neurons, n = 71, 0.62 ± 0.27; mean ± SD; p > 0.50). The response probability was also similar (RFP+ neurons, n = 24, 0.58 ± 0.27; RFP− neurons, n = 72, 0.59 ± 0.25; mean ± SD; p > 0.50). Finally, we compared the receptive field size and the response probabilities between MI-projecting neurons and SII-projecting neurons, measured in different animals. The ratio of the response probabilities to PW and SW stimulation was larger for MI-projecting neurons compared with SII-projecting neurons (p < 0.03). For the response probability to PW stimulation, the difference was not significant (p > 0.10). Importantly, for the RFP− neurons neither the receptive field size nor the response probability to PW stimulation were significantly different between the two sets of experiments (receptive field size, p > 0.80; response probability, p > 0.80).

Discussion

Primary sensory cortices send axons to multiple cortical and subcortical areas that process different aspect of sensory information (Felleman and Van Essen, 1991; Kaas et al., 1999; Alloway, 2008). Our results are consistent with the view that different targets receive distinct types of information from the same area (Movshon and Newsome, 1996; Morishima and Kawaguchi, 2006; Brown and Hestrin, 2009). We speculate that MI receives signals related to voluntary whisking, which might not require information about individual whiskers. In contrast, SII may be involved in object recognition and require information relating touch and whisker identity (Alloway, 2008).

Our study is the first demonstration of in vivo Ca imaging combined with retrograde tracing. This approach has advantages over extracellular recording combined with antidromic stimulation: it allows us to compare the activity of neighboring neurons simultaneously. Thus, the functional difference can be unambiguously assigned to projection patterns rather than differences in location (Ferraina et al., 2002) or the state of animals. In contrast, antidromic stimulation methods provide information about precise spike timing and can be applied to deep brain structures (Hikosaka and Wurtz, 1983).

Understanding the logic underlying the diversity of neural responses in the cortex is an essential step in revealing cortical computation. The technique used in the current study, along with related approaches (Sohya et al., 2007; Lima et al., 2009), will be useful in assigning other types of sensory responses to particular cell types defined by their axonal projection patterns.

Footnotes

  • We thank Susana Lima, Tony Zador, and Ed Callaway for information related to retrograde viruses, Patrick Kanold, Takaki Komiyama, Na Ji, and Mac Hooks for extensive discussions, Noah Gray, Chris Harvey, Mariko Izumo, Alla Karpova, and Tianyi Mao for advice on molecular cloning, Asaf Keller, Kevin Alloway, Ford Ebner, and Dan Simons for advice on anatomy and microstimulation, and Takaki Komiyama, Dan O'Connor, and Leopoldo Petreanu for comments on the manuscript.

  • Correspondence should be addressed to Takashi R. Sato, Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147. satot{at}janelia.hhmi.org

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The Journal of Neuroscience: 30 (12)
Journal of Neuroscience
Vol. 30, Issue 12
24 Mar 2010
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The Functional Properties of Barrel Cortex Neurons Projecting to the Primary Motor Cortex
Takashi R. Sato, Karel Svoboda
Journal of Neuroscience 24 March 2010, 30 (12) 4256-4260; DOI: 10.1523/JNEUROSCI.3774-09.2010

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The Functional Properties of Barrel Cortex Neurons Projecting to the Primary Motor Cortex
Takashi R. Sato, Karel Svoboda
Journal of Neuroscience 24 March 2010, 30 (12) 4256-4260; DOI: 10.1523/JNEUROSCI.3774-09.2010
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