Figure 1.
Stick-figure diagrams of the proteins examined, highlighting selected domains and mutations. Proteins are shown from N terminus (left) to C terminus (right), and the number of amino acids is shown at the right. For the Cdh23 proteins, the number of amino acids is for the precursor with the N-terminal signal peptide. Asterisk indicates the C-terminal PBM. A, For prestin, predicted transmembrane (TM) domains are gold, and the cytoplasmic tail (Cyto. tail) is purple. Upstream of the C-terminal transmembrane domain, predicted cytoplasmic segments are black, and predicted extracellular segments are gray. B, For Cdh23 and Cdh23 with exon 68 [Cdh23(+68)], the cadherin ectodomain repeats (R) are gray, the 35-residue exon 68 peptide (68) is gold, and the 35-residue harmonin binding site (b) in the cytoplasmic tail (Cyto. tail) is purple. The FLAG-tagged Cdh23 proteins examined are estimated to contain 11 full cadherin ectodomain repeats. PM, Plasma membrane. C, For harmonins a1 and b1, the N domain (N) is purple, and the three PDZ domains (PDZ1, PDZ2, and PDZ3) are gold. CC, Predicted coiled-coil domain; PST, domain rich in proline, serine, and threonine. D, For espin, the 116-residue C-terminal actin-bundling module (C-term. ABM) is purple and has putative F-actin binding sites (a1, a2) disposed at opposite ends. The GFP-tagged espins examined are splice-isoform 3A, which begins approximately midway between xAB and PR2, 59 aa upstream of PR2. AR, Ankyrin-like repeat; PR, proline-rich domain; xAB, additional F-actin binding site; WH2, Wiskott-Aldrich homology 2 domain. E, For myosin XVa, the motor domain is purple. The GFP-tagged myosin XVa proteins examined are isoform 2a, which is missing the N-terminal extension upstream of the motor domain. IQ, Two IQ domains; M4, MyTH4 (myosin tail homology 4) domain; F, FERM (band 4.1, ezrin, radixin, and moesin) domain; S, SH3 (Src homology 3) domain.