Intramembranous Valine Linked to Schizophrenia Is Required for Neuregulin 1 Regulation of the Morphological Development of Cortical Neurons

This work was supported by grants from the National Alliance for Research on Schizophrenia and Depression/Baer Foundation (Y.C., D.A.T., L.W.R.) and McKnight Brain Disorders (L.W.R.) and National Institutes of Health Grants DK07328 (M.L.H.) and NS29071 (L.W.R., D.A.T.). We thank Ping Ting Chen and Adnaan Sheriff for technical support and image analysis. Y.C. thanks David Lin for support and suggestions. Y.C. designed and performed all experiments in cortical neurons, analyzed data, and prepared this manuscript and figures. M.L.H. generated Gal4_DBD fusion proteins containing wild-type and mutant forms of mouse type III NRG1, performed the initial alanine scanning mutagenesis, prepared B4-ECD proteins, and tested the specificity of anti-CRD antibody in the spinal cord. D.A.T. performed experiments testing for the effectiveness of the ERBB kinase inhibitor in T47D cells. D.A.T. and L.W.R. provided valuable suggestions in the design of experiments and in data analysis and edited this manuscript.