Figure 3.
Magnitude of cooling-induced TRPA1 potentiation is dependent on the temperature and AITC concentration. A, B, Representative continuous whole-cell recordings at −60 mV from HEK-293 cells expressing rTRPA1 and perfused with Ca2+-free bath solutions containing AITC at 5 μm (A) or 50 μm (B). AITC was perfused at 30°C (red horizontal bars) or 20°C (open horizontal bars). Addition of 5 μm AITC caused a very slow activation of the current. For this reason, the early phase of current development is omitted in A. The 20 μm HC-030031 was perfused at the times indicated by the black horizontal bars. C, Quantification of the increase in inward current evoked by cooling over that evoked by AITC alone. Cooling from 30 to 20°C (black bars) or from 30 to 10°C (blue bars) is shown in the presence of varying concentrations of AITC. The bars show mean ± SEM of three (500 μm AITC) or four (50 and 5 μm AITC) cells from whole-cell recordings as shown in A and B.
