Figure 3.
The TRN dendritic/Ca2+ relationship is independent of dendritic size. A, Top, Stacked 2PLSM image of an example TRN neuron filled. Boxes indicate where line scans were performed. Bottom, Corresponding Ca2+ responses obtained from the neuron above. B, Left, Plot depicts Ca2+ responses obtained along 14 different TRN dendrites as a function of dendritic distance. C, Left, Correlation between TRN dendrite diameter and measured distance from soma. Right, Correlation between the peak Ca2+ response and the measured TRN dendritic diameter. D, Left, Correlation between dendrite diameter and measured distance from soma for both dLGN (blue) and VB neurons (red). Right, Correlation between Ca2+ response and the measured dendritic diameter for both thalamocortical projection neurons. E, Left, Diagram illustrating how data were pooled based on their radial distance from soma (soma, proximal, intermediate, distal I, and distal II). Right, Plot summarizing the average response for the three different thalamic cell types (dLGN: soma, 2.9 ± 2.5%, n = 7N; proximal, 7.9 ± 4.2%, n = 11D/7N; intermediate, 8.6 ± 4.2%, n = 13D/8N; distal I, 8.1 ± 3.2%, n = 9D/6N; VB: soma, 2.4 ± 1.0%, n = 4N; proximal, 5.9 ± 2.9%, n = 8D/4N; intermediate, 10.2 ± 2.7%, n = 8D/4N; distal I, 11.0 ± 3.0%, n = 7D/4N).
