Figure 2.
A, Quantification of histological sections stained with anti-GFAP at different distances from the lesion epicenter (0) show that tissue sparing was significant improved at the epicenter and areas rostral and caudal in mice treated with fenretinide (*p < 0.05). B, C, Representative micrographs showing GFAP immunostaining at the epicenter of the injury in vehicle (B)- and fenretinide (C)-treated mice at 28 dpi. Note the greater tissue sparing in mice treated with fenretinide. D, Graph showing that mice treated with fenretinide have greater neuron survival at distances of 300 and 500 μm rostral and 500 μm caudal to the lesion epicenter (*p < 0.05). Quantification done on tissue sections stained with NeuN. E, F, Micrographs showing NeuN staining of neurons in the ventral horn 500 μm rostral to the lesion epicenter in mice treated with vehicle (E) and fenretinide (F) at 28 dpi. Note the marked increased in neuronal profiles in mice treated with fenretinide. G, Mice treated with fenretinide display significantly greater serotonergic innervation in the ventral horns 1 mm caudal to the lesion epicenter (*p < 0.01). H, I, Representative micrographs showing 5-HT-immunoreactive fibers in the ventral horn at a distance 1000 μm caudal to the injury site in vehicle (H)- and fenretinide (I)-treated mice. A marked increased in serotonergic fibers is seen in the ventral horns of mice treated with fenretinide compared with vehicle-treated injured mice (n = 8 for each group). Scale bars: B, C, 500 μm; E, F, H, I, 100 μm. Error bars indicate SEM.