Figure 7.
Early patterning of the otocyst is not affected in the miR-183 family morphants. In situ hybridization and bright-field images of embryos at 24 hpf are shown. Uninjected controls, >20 embryos for each probe; 182/183 MO, 7–12 embryos for each probe; 96 MO, 16–25 embryos for each probe; all3 MO, 3–8 embryos for each probe. Purple signals show expression of egr2b (A) in the hindbrain and otx1 (B), dlx3b (C), hmx3 (D), and pax2a (E) in the otocyst. A, Dorsal view is shown, and anterior is left. B–D, Anterior is left, and dorsal is up. E, Anterior is left, and medial is up. Scale bars: A, 100 μm; B–E, 25 μm.