Figure 1.
Overexpression of dopamine D2R subunit in the striatum of Drd2-EGFP mice. A, Saturation binding isotherm for D2R using [3H]-methylspiperone performed on striatal membrane extracts (n = 12–14 mice/strain). B, Bmax values obtained from the radioligand binding assay. C, Kd values for D2R binding. D, E, Representative plots of qPCR assays (n = 8/genotype) using primer sets for the endogenous control β-actin gene “actb” (D) and drd2 (E). F, Drd2 mRNA levels expressed relative to wild-type mice. G, Linear representation of the clone RP23–161H15, which contains Drd2 (red), Ankk1 (black), and Ttc12 (blue). Exons and introns are represented by solid and open boxes, respectively. H, Inferred schematic of GENSAT-BX37 containing EGFP downstream of the Drd2 start codon. I, Enlarged diagram of upstream and downstream sequences around EGFP (green) deducted from the sequencing results. Left, Upstream sequence aligns with intron 1 of Drd2 (red). Right, Downstream sequence aligns with the C terminus of EGFP (green), a part of the insertion cassette (polyA, in gray), and intergenic sequence between Ankk1 and Ttc12 (red). *p < 0.05 compared with wild type.