Constancy and Variability in the Output of a Central Pattern Generator

Accounting for all the inhibitory synaptic inputs to a HE(10) motor neuron. An HE(10) motor neuron was recorded in voltage clamp (holding potential −45 mV) simultaneously with extracellular recording from four ipsilateral premotor heart interneurons, HN(3), HN(4), HN(6), and HN(7). Here and throughout interneurons and motor neurons are indexed by segmental ganglion number, thus HN(7) is the heart interneuron of segmental ganglion 7, and all recordings are ipsilateral. One to three bursts of HN firing were analyzed in each preparation: in this preparation three were used. In the portion of one burst illustrated (preparation was in synchronous mode here), standard symbols and colors are used to plot each HN spike, and dropped dashed lines from each HN spike symbol indicate the associated IPSC in the HE current trace. For clarity only a short portion of the trace associated with the HN burst is illustrated. 100% of the recorded IPSCs over 3 bursts were matched by a spike recorded from one of the HN interneurons with the correct latency in this HE(10) motor neuron. HN spikes from 11 bursts (including the one illustrated) from each interneuron were also used to generate the spike-triggered averages of IPSCs in the motor neuron shown on the lower right. The vertical dashed line indicates the time of the triggering interneuronal spike, standard colors identify each spike-triggered average, and standard symbols indicate the latency of the peak average IPSC from this triggering event. Note that the amplitude of the individual IPSCs from each identified HN spike match the amplitude expected from the spike-triggered average.

Determining the absolute and relative synaptic strength of each input to a heart motor neuron. In the top, an HE(12) motor neuron was recorded in voltage clamp (holding potential, −45 mV) simultaneously with extracellular recording from four ipsilateral premotor heart interneurons, HN(3), HN(4), HN(6), and HN(7). HN spikes from 11 bursts (including the ones illustrated) from each interneuron were used to generate the spike-triggered averages of IPSCs in the HE(12) motor neuron, and subsequently similar recordings were used to generate the spike-triggered averages of IPSCs for the HE(8) motor neuron in the same preparation. Upward arrows indicate the time of the triggering HN spike, and the downward arrows indicate the peak of the averaged IPSC used to measure amplitude: standard colors identify each heart interneuron and associated spike-triggered average IPSC. Iconic unilateral circuit diagram (bottom right) identifies the recorded neurons. Preparations are specified by the day on which they were recorded: letters accompany the day designation if more than one preparation was recorded on that day.

Whisker-box diagram of absolute synaptic strength of inputs to the HE(8), HE(10), and HE(12) motor neurons determined as illustrated in Figure 3. Iconic unilateral circuit diagram (above) identifies the recorded neurons for each diagram, and standard colors are used to indicate the strength of each HN input in each diagram. Each box shows the median value at its waist and the third and first quartile at its top and base, respectfully. The whisker ends indicate the lowest datum still within 1.5 * IQR (interquartile range) of the lower quartile, and the highest datum still within 1.5 * IQR of the upper quartile with outliers indicated by red crosses. The strength profile across the four inputs, HN(3), HN(4), HN(6), and HN(7) interneurons, is distinctive for each motor neuron but with considerable variability in the strength of each input in each motor neurons across animals (n).

Viewing the animal-to-animal variation in absolute synaptic strength illustrated in Figure 3. Iconic unilateral circuit diagram (above) identifies the recorded neurons for each graph, and standard colors and symbols are used to indicate the strength of each HN input in each graph. Absolute strength (in nanosiemens; top graphs) or relative strength (bottom graphs) is plotted versus preparation ordered by the absolute or relative strength of the HN(3) input, respectively.

Determining the input and output temporal pattern of the HE(8) and HE (12) motor neurons. Left, Simultaneous extracellular recordings were made of ipsilateral HN(3), HN(4), HN(6), and HN(7) premotor interneurons (inputs) (standard color code) and HE(8) and HE (12) motor neurons (outputs) (black) in both peristaltic (P) and synchronous (S) coordination modes. Right, Summary phase diagram of the premotor interneurons (standard color code) and the HE(8) and HE(12) motor neurons in both the peristaltic (pink-outlined boxes) and synchronous (light blue-outlined boxes) coordination modes. For both motor neurons and interneurons, the average duty cycle is indicated by the length of the bar: the left edge of each bar indicates the average phase of the first spike of the burst, and the right edge indicates the average phase of the last spike of the burst. Average phase is indicated by a vertical line within the bar. Error bars indicate SDs. A phase diagram of the interneurons was first constructed from measurements of activity phase relative to the middle spike of the ipsilateral HN(4) interneuron for both synchronous and peristaltic coordination modes. To align these ipsilateral phase diagrams, the synchronous HN(4) interneuron was assigned a phase of 0.511, as measured with respect to the peristaltic HN(4) interneuron in bilateral recordings (Norris et al., 2006). All other synchronous interneurons were then offset by the same amount as the phase of the synchronous HN(4) interneuron.

Whisker-box diagrams of absolute and relative synaptic strength of inputs to the HE(8) and HE(12) motor neurons. Data were drawn from a series of 12 preparations in which the input and output temporal pattern of the HE(8) and HE(12) motor neurons was completely specified as illustrated in Figure 6 and in which the strength pattern of the inputs to both motor neurons was also measured, as illustrated in Figure 3. The strength profile across the four inputs, HN(3), HN(4), HN(6), and HN(7) interneurons (standard color code used), is similar to that for the larger population illustrated in Figure 4, indicating that our sample is representative.

Complete analysis of input and output temporal patterns and synaptic strength patterns (spatial pattern) for three different preparations from our sample of 12 (summarized in Fig. 7). Temporal patterns were determined as in Figure 6, but the two ipsilateral phase diagrams were fused into a composite for ease of presentation. The strength pattern of the inputs, determined as in Figure 3, is shown to the right of each composite phase diagram. Standard colors and symbols are used throughout. Preparations are specified by the day on which they were recorded: letters accompany the day designation if more than one preparation was recorded on that day.

Complete analysis of input and output temporal patterns and synaptic strength patterns (spatial pattern) for three different preparations from our sample of 12 (summarized in Fig. 7). Temporal patterns were determined as in Figure 6, but the two ipsilateral phase diagrams were fused into a composite for ease of presentation. The strength pattern of the inputs, determined as in Figure 3, is shown to the right of each composite phase diagram. Standard colors and symbols are used throughout. Preparations are specified by the day on which they were recorded: letters accompany the day designation if more than one preparation was recorded on that day.