Figure 3. Radioactive in situ for TRPV1 in DRGs and brain of mouse, rat, and human. A, Bright-field image of radioactive in situ in mouse DRG, counterstained with H&E. B, Double labeling with TRPV1 antibody (brown) and radioactive in situ probe (black) shows complete colocalization between the two in mouse DRG. C, Bright-field image of radioactive in situ in rat DRG, counterstained with H&E. S, Small-diameter neurons; M, medium-diameter neurons; L, large-diameter neurons. Asterisks indicate negative cells. D, Dark-field image of radioactive in situ in rat DRG. E–H, Coronal sections of rat brain processed for radioactive TRPV1 in situ hybridization showing positive signals in PH (E), SuM (F), IF (G), and DMV (10th motor nucleus; H). I–P, Close-up images of indicated regions are shown for rat (I–L) and mouse (M–P). Q, Dark-field image of radioactive in situ signal in human DRG. R, S, Representative images of radioactive in situ in human brain sections, demonstrating complete lack of staining. ac, Anterior commissure; CA1, CA1 region of hippocampus; cc, corpus callosum; Cd, caudate; CI, claustrum; DG, dentate gyrus; ec, external capsule; GP, globus pallidus; ins gyrus, insular gyrus; Pir Cx, piriform cortex; Put, putamen. Scale bars: A, B, Q, 100 μm; C, D, I–L, 50 μm; E–H, 2 mm; M–P, 25 μm; R, S, 5 mm.