Figure 8.
Kir2.1 overexpression impairs functional connectivity of newborn DGCs in running mice. A, Analysis of mEPSCs for 21 dpi neurons. Left panels show example voltage-clamp whole-cell recordings obtained from a GFP-expressing neuron (control, black) and a Kir-overexpressing neuron (blue). Right panels depict quantitative analysis of mEPSC amplitude and frequency; n = 8 (control) and n = 14 (Kir). *p = 0.025 (21 dpi, amplitude) and p = 0.012 (frequency), t tests. Calibration: 5 pA, 1 s. B, Analysis of mEPSCs for 35 dpi neurons, with n = 14 for both control and Kir neurons. **p = 0.0094, Mann–Whitney test. Calibration: 5 pA, 1 s. C, Analysis of mIPSCs for 35 dpi neurons, with n = 12 (control) and n = 8 (Kir). **p = 0.0018, Mann–Whitney test. Calibration: 20 pA, 1 s. D, Voltage-clamp measurements of membrane capacitance (Cm) for 21 and 35 dpi neurons expressing GFP alone (control), Kir2.1 (Kir), and a nonconductive mutant version of Kir (KirNC). A significant reduction of Cm was induced by Kir but not KirNC at both time points. Data for 35 dpi were transformed to reach normality using a natural log function. **p < 0.01 and ***p < 0.001 by ANOVA with Bonferroni's post hoc test, with n = 41 (21 dpi, control), 41 (21 dpi, Kir), 17 (21 dpi, KirNC), 15 (35 dpi, control), 15 (35 dpi, Kir), and 11 (35 dpi, KirNC). All bar charts represent mean ± SEM.