Figure 5. mGluR-LTD is selectively disrupted in Tsc1 KO neurons. A–D, Mean EPSC amplitude ± SEM expressed as a percentage of baseline plotted versus time in minutes. Dashed lines indicate the average baseline response. Insets show representative EPSCs. Black traces represent the average response from the 10 min baseline period (1). Gray traces represent the average response during the last 10 min (2). A, B, mGluR-LTD was induced by a 5 min wash-in of 100 μm DHPG. A, Tsc1fl/fl mice: control, CreEGFP-negative neurons (n = 9 cells); Tsc1 KO, CreEGFP-positive neurons (n = 8 cells). B, WT-Cre+, CreEGFP-positive neurons from C57BL/6 mice (n = 8). C, mGluR-LTD was induced by PP-LFS, consisting of 900 pairs of stimuli (50 ms ISI) delivered at 1 Hz in the presence of d-APV (n = 10). D, NMDA-dependent LTD was induced by LFS consisting of 5 Hz stimulation for 3 min at −40 mV (n = 8–9). E, Summary data showing the mean ± SEM response measured during the last 10 min of each recording expressed as a percentage of the 10 min baseline period for the indicated genotypes. Dashed line at 100% indicates the baseline. Left bars (DHPG) represent data from A and B, middle bars (PP-LFS) are from C, right bars (LFS) are from D. *Significant difference from 100%, p < 0.01.