Unusual Pungency from Extra-Virgin Olive Oil Is Attributable to Restricted Spatial Expression of the Receptor of Oleocanthal

This work was supported by National Institutes of Health (NIH) Grant DC02995 (P.A.S.B.), NIH Grant DC006760 (P.A.S.B., G.K.B.), and NIH Grant GM29028 (A.B.S.). J.B.S. was supported by NIH–Ruth L. Kirschstein National Research Service Award FCA121716A. Confocal microscopy was supported by National Science Foundation Grant DBI-0216310 (N. Rawson). TRPA1 knock-out mice were generously provided by David Corey and Kelvin Kwan from Harvard University (Cambridge, MA). The following reagents were provided as gifts: human TRPA1 (gift from Dr. A. Patapoutian, The Scripps Institute, La Jolla, CA), human TRPV1 (from Dr. Y. Mori, Kyoto University, Kyoto, Japan), human TRPV2 (from Dr. S. Wakabayashi, National Cardiovascular Center Research Institute, Osaka, Japan), human TRPV4 (from Dr. W. Leidtke, Duke University, Durham, NC), rat TRPV1, rat TRPV2, rat TRPM8 (from Dr. D. Julius, University of California, San Francisco, San Francisco, CA), and rat TRPV4 or mouse TRPV3 (from Dr. M. Caterina, The Johns Hopkins University, Baltimore, MD). We thank N. Rawson and K. Yee for assistance with nasal biopsy tissue and H. Ozdener for help with immunohistochemistry experiments. We also thank K. Plank for assistance with mouse colony maintenance.