Figure 3. The AFD, AWC, and ASI neurons are required in a degenerate manner to generate negative thermotaxis bias under different conditions. A, B, Thermotaxis bias of wild-type and neuron-ablated strains (see Materials and Methods) under the indicated conditions. Wild-type data from Figure 1 are included for comparison. Data from wild-type, tax-4, and neuron-ablated strains shown in Figures 1B, 1C, 3A, and 3B were obtained together on multiple days. For each data point, n = 105 animals; 7 independent assays. *, **, and *** indicate significant differences from wild-type values at p < 0.05, p < 0.01, and p < 0.001, respectively, by one-way ANOVA with Bonferroni post hoc correction. Conditions examined further are shaded. C–E, Thermotaxis bias of indicated strains under the specified conditions. EC indicates that the promoter::caspase constructs were driven under different promoter combinations and present as extrachromosomal arrays (see Materials and Methods). n = 105 animals; 7 independent assays. *, **, and *** indicate significant differences from wild-type values at p < 0.05, p < 0.01, and p < 0.001, respectively, by one-way ANOVA with Bonferroni post hoc correction. Error bars are the SEM. Average speeds (in millimeters per second) of strains were as follows: (Tc = 15°C) wild-type—0.17 ± 0.008, AFD-ablated—0.13 ± 0.002, AWC-ablated—0.17 ± 0.007, ASI-ablated—0.15 ± 0.005; (Tc = 20°C) wild-type—0.18 ± 0.005, AFD-ablated—0.14 ± 0.004, AWC-ablated—0.20 ± 0.003, ASI-ablated—0.15 ± 0.004. F, Thermotaxis bias of wild-type or AFD-ablated strains expressing pkc-1(gf) under cell-specific promoters from extrachromosomal arrays. Numbers shown are from one transgenic line each. n = 105 animals; 7 independent assays. * and *** indicate significant differences at p < 0.05 and p < 0.001, respectively, between values indicated by brackets (one-way ANOVA with Bonferroni post hoc correction). Error bars are the SEM.