Figure 2. Reduced response to CIO stimulation in 6- and 12-week-old ATXN1[82Q] mice. A, Background images of exposed cerebella (top row), pseudocolored images from individual animals (middle row), and compilation images from each genotype (bottom row) demonstrate characteristic response patterns to CIO stimulation. B, Time courses for single trials illustrate typical changes in flavoprotein autofluorescence over the duration of CIO stimulation (horizontal bar). C, Representative FPs evoked by CIO stimulation for each genotype. The stimulation artifact is shown only for WT/FVB. D, E, Quantification of CF-mediated flavoprotein autofluorescence responses and CF-N1 of the FP. WT/FVB: N = 11 animals; n = 60 observations for both autofluorescent flavoprotein imaging (AFI) and FPs; 6-week-old WT/FVB mice: N = 5, n = 40 for AFI; and N = 4, n = 32 for FPs; Atxn1−/− mice: N = 4, n = 32; ATXN1[30Q] mice: N = 8, n = 60 for AFI; and N = 7, n = 51 for FPs; 6-week-old ATXN1[82Q] mice: N = 4, n = 32; 12-week-old ATXN1[82Q] mice: N = 4, n = 32; ATXN1[82Q]-K772T mice: N = 4, n = 32. Boxes denote significant differences.