Abstract
The role of Ca2+ in synaptic vesicle endocytosis remains uncertain due to the diversity in various preparations where several forms of endocytosis may contribute variably in different conditions. Although recent studies have demonstrated that Ca2+ is important for clathrin-mediated endocytosis (CME), the mechanistic role of Ca2+ in CME remains to be elucidated. By monitoring CME of single vesicles in mouse chromaffin cells with cell-attached capacitance measurements that offer millisecond time resolution, we demonstrate that the dynamics of vesicle fission during CME is Ca2+ dependent but becomes Ca2+ independent in synaptotagmin 1 (Syt1) knock-out cells. Our results thus suggest that Syt1 is necessary for the Ca2+ dependence of CME.
Introduction
Neurotransmitter release is mediated by exocytosis of synaptic vesicles, and subsequent endocytosis is essential for supporting rapid and repeated rounds of release (Stevens, 2003). Although the roles of Ca2+ in exocytosis are well established (Zucker and Regehr, 2002; Neher and Sakaba, 2008), its role in synaptic vesicle endocytosis remains uncertain (Ceccarelli and Hurlbut, 1980; Ramaswami et al., 1994; von Gersdorff and Matthews, 1994; Cousin and Robinson, 1998; Gad et al., 1998; Marks and McMahon, 1998; Beutner et al., 2001; Neves et al., 2001; Sankaranarayanan and Ryan, 2001; Wu et al., 2005), largely because the results are diverse among various preparations where several forms of endocytosis may contribute variably in different conditions (Wu et al., 2007). It has been recently demonstrated that Ca2+ is critical in clathrin-mediated endocytosis (CME), the slow form of endocytosis, in the calyx of Held (Hosoi et al., 2009; Wu et al., 2009). However, it remains to be shown how Ca2+ regulates the process of CME and which molecules determine the Ca2+ dependence of CME. In the present study, CME of single vesicles was monitored using cell-attached capacitance measurements, in which a sinusoidal voltage was superimposed to the holding potential and a two-phase lock-in amplifier was used to analyze the current output signal. When the phase of the lock-in amplifier was properly adjusted, the two outputs of the amplifier directly provided the changes of membrane conductance in one channel and the changes of membrane capacitance in the other channel (Neher and Marty, 1982; Debus and Lindau, 2000). When the tubular membrane neck that connects the endocytic vesicle to the plasma membrane before vesicle pinch-off has a low conductance, the vesicle capacitance (Cv) is not fully charged and the values in the capacitance trace are reduced while a transient increase in the conductance trace appears. As for exocytotic fusion pores (Debus and Lindau, 2000), the analysis of these changes can be used to estimate the dynamic behavior of endocytic fission-pore conductance (Gp) (Rosenboom and Lindau, 1994; Dernick et al., 2003; MacDonald et al., 2005; Zhao et al., 2010). Our results demonstrate that the dynamics of vesicle fission during CME is Ca2+ dependent but becomes Ca2+ independent in synaptotagmin 1 (Syt1) knock-out (KO) cells, indicating that Syt1 is required for the Ca2+ dependence of CME.
Materials and Methods
Chromaffin cell culture.
Adrenal glands of newborn pups of either sex (postnatal day 0) from wild-type (WT) or Syt1 heterozygous (HT) mouse matings were isolated in accordance with the guidelines of the National Institutes of Health, as approved by the Animal Care and Use Committee of the University of Illinois at Chicago. Tails were kept for genotyping for newborn pups from Syt1 HT matings, and electrophysiological recordings on chromaffin cell cultures from littermate WT and Syt1 KO mice were compared. WT chromaffin cells were typically from WT matings, except that WT chromaffin cells for data presented in Figure 3 were from Syt1 HT matings.
Mouse chromaffin cells were prepared and cultured as previously described (Gong et al., 2005). The following solutions were prepared and sterile filtered (0.22 μm): papain solution, 250 ml of DMEM (Invitrogen) was supplemented with 50 mg of l-cysteine/1 mm CaCl2/0.5 mm EDTA/20–25 U/ml papain (Worthington), and equilibrated with 5% CO2; inactivating solution, 225 ml of DMEM was supplemented with 25 ml of heat-inactivated FCS/625 mg of albumin/625 mg of trypsin inhibitor (Sigma); enriched DMEM, 500 ml of DMEM was supplemented with 5 ml of penicillin/streptomycin (Invitrogen)/5 ml of insulin-transferrin-selenium-X (Invitrogen); and Locke's solution, 154 mm NaCl/5.6 mm KCl/3.6 mm NaHCO3/10 mm glucose, pH 7.3 with NaOH. Adrenal glands were dissected from newborn mice and placed in filtered Locke's solution. Contaminating tissue was removed by dissection. The glands were incubated in 1 ml of papain solution at 37°C for 40 min and inactivated by addition of 0.75 ml of the inactivating solution for another 10 min. The medium was carefully replaced with 0.2 ml of enriched DMEM, and the glands were triturated gently through a 200 μl pipette tip. Seventy microliters of the cell aliquots were plated on 12 mm coverslips coated with poly-d-lysine (Sigma), and cells were allowed to attach before supplemented with 2 ml of enriched medium. The cells were incubated at 37°C in 5% CO2 and used within 4 d.
Solutions.
The bath solution contained 140 mm NaCl, 5 mm KCl, 2 mm CaCl2, 1 mm MgCl2, 10 mm HEPES-NaOH, and 10 mm glucose; the pH was adjusted to 7.3 with NaOH, and the osmolarity was ∼310 mmol kg−1. The solution in the cell-attached pipette contained 50 mm NaCl, 100 mm TEACl, 5 mm KCl, 2 mm CaCl2, 1 mm MgCl2, and 10 mm HEPES-NaOH; the pH was adjusted to 7.3 with NaOH, and the osmolarity was adjusted to 290 mmol kg−1. The solution in the whole-cell pipette for the double (cell-attached/whole-cell) patch approach contained 20 mm CsCl, 100 mm KCl, 20 TEACl, 1 mm MgCl2, 10 mm HEPES, 2 mm MgATP, and 0.3 Na3GTP; the pH was adjusted to 7.3 with KOH, and the osmolarity was adjusted to 290 mmol kg−1. Basal [Ca2+]i in the whole-cell pipette solutions was buffered by 1.1 mm CaCl2 and 2 mm EGTA to give a final free [Ca2+]i of ∼150 nm.
Cell-attached capacitance recordings.
Changes in membrane capacitance and conductance were recorded by the cell-attached capacitance technique of patch-clamp (Debus and Lindau, 2000; Dernick et al., 2005). Patch pipettes were pulled in four stages with a programmable P-97 puller (Sutter Instruments) and coated with a sticky wax (Kerr). Pipettes were fire polished and had a typical resistance of ∼2 MΩ in the bath solution. For cell-attached capacitance measurements, an EPC-7 plus patch-clamp amplifier (HEKA Elektronik) was used. Command voltage was applied to the bath solution. Changes of patch admittance were measured, as described previously (Debus and Lindau, 2000), with a SR830 lock-in amplifier (Stanford Research Systems) using a sine-wave amplitude of 50 mV (root mean square) at a frequency of 20 kHz. The output filter of the lock-in amplifier was set to a 1 ms time constant, 24 db. The phase of the lock-in amplifier was set such that transient capacitance changes produced by gentle suction pulses appeared only in the patch capacitance (Im) trace, with no projection into the patch conductance (Re) trace (Debus and Lindau, 2000). Because the phase may change during the recording, the phase for sections with endocytic events of interest was readjusted such that the baseline of the Re trace was at the same level before and after completion of the capacitance step.
All endocytic events were recorded by the cell-attached patch configuration unless mentioned otherwise. Capacitance steps and fission-pore durations were reliably detected for step sizes >0.2 fF, and smaller steps were not included in the analysis. The number of endocytic events per patch detected in the cell-attached recordings was counted as the total number of downward capacitance steps within the first 5 min of recordings.
Double (cell-attached/whole-cell) patch recordings.
The endocytic events were also recorded by the double (cell-attached/whole-cell) patch configuration in some experiments. In this double-patch configuration, the cells held at −65 mV were stimulated by a train of 100 ms depolarization to 0 mV at 0.2 Hz through the whole-cell pipette to induce endocytosis of single vesicles that were recorded by the other cell-attached patch pipette. The number of endocytic events recorded per patch in this double (cell-attached/whole-cell) patch configuration was counted as the total number of downward capacitance steps >0.2 fF within 5 min of the first depolarization.
Fission-pore analysis.
Fission-pore closures of endocytic events were analyzed in the same way as exocytotic fusion-pore openings as described previously (Debus and Lindau, 2000; Dernick et al., 2005; Gong et al., 2007). During fission-pore closure of endocytic events in which a transient increase in the Re signal was associated with a decline in the Im step, the Cv was determined as the total change in the Im trace for a particular event and the Gp was calculated using the formula
Amperometry.
Conventional carbon fiber amperometry for catecholamine detection used 5 μm carbon fibers (ALA Scientific Instruments) as described previously (Gong et al., 2005, 2007). The freshly cut tip of the carbon fiber electrode was positioned closely against the cell surface to minimize the diffusion distance from release sites. The amperometric current, generated by catecholamine oxidation at the exposed tip of the carbon fiber electrode, was measured using an EPC-7 plus amplifier, operated in the voltage-clamp mode at a holding potential of +700 mV. Amperometric signals were low-pass filtered at 1 kHz and digitized at 4 kHz. Similar to the stimulation pattern in double-patch configuration, secretion was induced by a train of 100 ms depolarization to 0 mV at 0.2 Hz through a whole-cell patch pipette. Amperometric recordings were collected and then analyzed with a customized macro for IGOR software (WaveMetrics) to extract spike information according to the criteria of Chow et al. (1992). The number of amperometric spikes was counted as the total number of spikes with an amplitude >10 pA within 120 s of the first depolarization.
Reagents.
Anti-clathrin heavy chain antibody (X22) was obtained from Affinity BioReagents. The KR-peptide, a Syt1 C2B domain-derived peptide with the sequence of KRLKKKKTTIKK, was used to block the interaction of Syt1 and AP-2 (the clathrin adaptor protein complex) (Grass et al., 2004). The control experiments were performed using the scrambled version of the KR-peptide (Scr-peptide, KKKRTLTKKKKI) and the mutant version of the KR-peptide (AA-peptide, KRLKKAATTIKK) with two of the conserved lysines (KK) replaced by alanines (AA), which displays no interaction with AP-2, as shown by a previous study (Kastning et al., 2007). These three peptides were synthesized by Biomatik and NeoBioscience. The intracellular application of anti-clathrin heavy chain antibody, KR-peptide, Scr-peptide, or AA-peptide was through a whole-cell patch pipette for at least 15 min in the double (cell-attached/whole-cell) patch configuration while endocytosis of single vesicles was recorded by the other cell-attached patch pipette.
Statistical analysis.
All the experiments were performed at room temperature. Data are expressed as mean ± SEM, and Newman one-way ANOVA tests were used for statistical analysis. Because typically one or two analyzable endocytic events can be obtained from each individual cell, every endocytic event was treated as statistically independent and the number of events was used for statistical testing of the fission-pore kinetics.
Results
Figure 1A shows a recording with both upward and downward capacitance steps. In this recording, the upward capacitance steps, which are associated with exocytosis as reported previously (Gong et al., 2003, 2007), occurred at earlier times than the downward capacitance steps associated with endocytosis (Fig. 1A). The plot of endocytic events, as a function of time, shows that the frequency of endocytic events increased after the first depolarization (Fig. 1B), suggesting that the stimulation effectively increased the rate of endocytotic events. The diameter of individual vesicles associated with endocytosis was calculated assuming a specific capacitance of 9 fF/μm2 (Albillos et al., 1997; Gong et al., 2003) and spherical geometry. The resulting frequency distribution of vesicle diameters for these endocytic events did not show evidence of a multimodal distribution (Fig. 1C), suggesting that the downward capacitance steps reflect a single type of endocytic events. Figure 1D exemplifies a cell-attached recording with Re (top) and Im (bottom) in a control chromaffin cell. This trace displays five downward capacitance steps, indicating sequential endocytosis of five individual vesicles. When an anti-clathrin heavy chain antibody was delivered into the cells through the whole-cell pipette in double (cell-attached/whole-cell) patch configuration, the number of endocytic events detected by the cell-attached pipette was significantly decreased in a concentration-dependent manner (p < 0.01) while control IgG had no significant effect (Fig. 1E,F), demonstrating that these endocytic events represent CME. For a subset of individual endocytic event, the decrease in Im was associated with a detectable transient change in the Re trace, reflecting detection of a narrow fission-pore (Fig. 1G), which was characterized by the fission-pore Gp and duration (Fig. 1H). We then examined the fission-pore kinetics of endocytic events in cells treated with 60 μg/ml anti-clathrin heavy chain antibody, which inhibited the number of endocytic events by ∼65% (Fig. 1F), because treatments with this antibody at concentrations of 120 or 240 μg/ml reduced the occurrence of endocytic events to such a low level that a statistical analysis was impossible. Our results showed that cells pretreated with 60 μg/ml antibody displayed no changes in the capacitance step size of the endocytic vesicles (Fig. 1I), the fission-pore Gp (Fig. 1J), or the fission-pore duration (Fig. 1K). It has been shown by electron microscopy that AP-2 and associated endocytic accessory proteins assemble into coated membrane subdomains that lack any significant curvature in the absence of clathrin, demonstrating that clathrin is important for invagination during CME (Nossal, 2001; Hinrichsen et al., 2006). Our results thus indicate that the fission-pore kinetics cannot be affected by defect in endocytic invagination.
The endocytic events observed in the cell-attached recordings are Ca2+ dependent and triggered by Ca2+ influx into the cell during patching, because removal of external Ca2+ substantially reduced the occurrence of these events (p < 0.01) (Fig. 2A). The number of endocytic events was decreased by ∼70% at 0.5 mm [Ca2+]e compared to 2 mm [Ca2+]e (p < 0.01) (Fig. 2A). Meanwhile, exocytosis, as measured by carbon fiber amperometry, was also inhibited to a similar extent at 0.5 mm [Ca2+]e (p < 0.01) (Fig. 2B). As a result, the endocytosis/exocytosis ratio was indistinguishable at 2 and 0.5 mm [Ca2+]e (p > 0.05) (Fig. 2C), suggesting that the inhibition in endocytosis at lower [Ca2+]e may be a consequence of the reduction in exocytosis. The capacitance step size (Fig. 2D,E) and the fission-pore Gp (Fig. 2D,F) of these endocytic events were not statistically different in these two groups (p > 0.05). However, the fission-pore duration was prolonged by ∼250% at 0.5 mm [Ca2+]e, compared to 2 mm [Ca2+]e (p < 0.01) (Fig. 2D,G), indicating a direct role of Ca2+in vesicle fission during CME.
Given the Ca2+ binding capability of Syt1 and its putative role as the Ca2+ sensor in exocytosis (Chapman, 2008; Pang and Südhof, 2010), we tested whether Syt1, the predominant synaptotagmin isoform in chromaffin cells (Marquèze et al., 1995), may be responsible for the Ca2+ sensitivity of fission-pore dynamics during CME by examining the fission-pore kinetics of endocytic events in Syt1 KO cells (Fig. 3A). There was a significant decrease in the number of endocytic events in KO cells compared to WT cells (p < 0.01) (Fig. 3B). Although exocytosis, as measured by carbon fiber amperometry, was also inhibited (p < 0.01) (Fig. 3C), the endocytosis/exocytosis ratio was decreased in KO cells (p < 0.01) (Fig. 3D), pointing to a direct role of Syt1 in CME beyond exocytosis. Both the capacitance step size of endocytic vesicles (Fig. 3A,E) and the fission-pore Gp (Fig. 3A,F) were indistinguishable between WT and KO cells (p > 0.05). However, the fission-pore duration was increased by ∼150% in KO cells compared to WT cells (p < 0.01) (Fig. 3A,G). Comparison of the fission-pore durations at 2, 1, and 0.5 mm [Ca2+]e between WT and KO cells revealed that the Ca2+ dependence of the fission-pore duration observed in WT cells was abolished in Syt1 KO cells (Fig. 3H), indicating that the Ca2+ dependence of fission dynamics during CME requires Syt1.
Because Syt1 interacts with the endocytic adaptor AP-2 (Zhang et al., 1994; Chapman et al., 1998; Haucke and De Camilli, 1999; Grass et al., 2004), we tested whether the alterations in the endocytosis/exocytosis ratio and in the fission-pore kinetics in Syt1 KO cells are mediated by this interaction. Disruption of Syt1–AP-2 binding was fashioned using a peptide derived from the Syt1 C2B domain (KR-peptide with the sequence of KRLKKKKTTIKK), which is essential for the Syt1–AP-2 interaction (Grass et al., 2004). When the KR-peptide was delivered into the cells through the whole-cell patch pipette in the double (cell-attached/whole-cell) patch configuration, the number of endocytic events recorded at 2 mm [Ca2+]e was inhibited in a concentration-dependent manner (p < 0.01) (Fig. 4A). On the other hand, carbon fiber amperometry experiments showed that this KR-peptide had no effects on exocytosis (Fig. 4B), indicating a specific role of the Syt1–AP-2 interaction in endocytosis. As a consequence, the endocytosis/exocytosis ratio was reduced in cells treated with this KR-peptide (p < 0.01) (Fig. 4C). Both a scrambled version of the KR-peptide (Scr-peptide) and a mutant version of the KR-peptide, which displayed no interaction with AP-2 (Kastning et al., 2007) (AA-peptide), had no significant effects on endocytosis (Fig. 4A), exocytosis (Fig. 4B), or the endocytosis/exocytosis ratio (Fig. 4C) (p > 0.05), thus supporting the specificity of the KR-peptide. These results thus suggest that the reduction in the endocytosis/exocytosis ratio in Syt1 KO cells (Fig. 3D) may be likely due to the disrupted Syt1–AP-2 interaction.
To determine whether the KR-peptide changes the fission-pore kinetics, the endocytic events measured at 25 μm peptide concentration were analyzed because too few endocytic events could be obtained in cells dialyzed with this peptide at 100 μm (Fig. 4A). The capacitance step size of the endocytic vesicles (Fig. 4D) and the fission-pore Gp (Fig. 4E) were indistinguishable between control cells and cells treated with the KR-peptide (p > 0.05). Notably, and in contrast to Syt1 KO cells, the KR-peptide had no effect on the fission-pore duration at 2 mm [Ca2+]e (p > 0.05) (Fig. 4F). Moreover, in both control cells and cells treated with 25 μm KR-peptide, the fission-pore duration was increased in a similar fashion by a factor of ∼2 when lowering [Ca2+]e from 2 to 1 mm (Fig. 4F). Meanwhile, treatments with the Scr-peptide and the AA-peptide did not alter the capacitance step size of the endocytic vesicle (Fig. 4D), the fission-pore Gp (Fig. 4E), or the fission-pore duration (Fig. 4F) at both 2 and 1 mm [Ca2+]e (p > 0.05). These results indicate that, with KR-peptide at a concentration that produces partial inhibitions, the remaining CME events were still mediated by Syt1 while a higher peptide concentration resulted in full inhibitions. In contrast, inhibition was incomplete in Syt1 KO but the remaining events showed Ca2+-independent fission-pore dynamics. The most likely explanation for these results is that, in Syt1 KO cells, CME is mediated by other synaptotagmin isoforms with presumably no or low Ca2+ affinity than Syt1.
Discussion
In the present study, we monitored CME of single vesicles by two different configurations: cell-attached patch configuration, where endocytic events may be spontaneous and/or triggered by mechanical stimulation during patching, and the double (cell-attached/whole-cell) patch configuration, where endocytic events are stimulated by a train of depolarizations applied through the whole-cell patch pipette. The number of exocytotic events, which coexist with these endocytic events, as shown in Figure 1A, was increased in the double (cell-attached/whole-cell) patch configuration compared to cell-attached patch configuration (data not shown), indicating that the depolarizations were effective in stimulating exocytosis. Our results showed that the frequency of endocytic events was also increased after stimulation (Fig. 1B). It may, therefore, be expected that the number of endocytic events recorded in the double-patch configuration would be higher than that in the cell-attached patch configuration. However, contrary to this expectation, our results showed that the numbers of endocytic events recorded in the two configurations were comparable (Figs. 1F, 2A, 3B, 4A). The number of endocytic events in the double-patch configuration could possibly be lowered due to a loss of some factors necessary for CME, when the cells are infused with intracellular solution in the whole-cell pipette. Consistent with this implication, a previous study has demonstrated that compensatory endocytosis, which occurs consistently in perforated patch configuration, shows rapid wash-out in the whole-cell configuration (Smith and Neher, 1997).
We have shown here that the dynamics of fission-pore closure in chromaffin cells is Ca2+ dependent, which is consistent with Ca2+-dependent fission-pore kinetics in insulin-secreting INS-1 cells (MacDonald et al., 2005). In insulin-secreting cells, fission-pore kinetics exhibit two distinct kinetic components (Zhao et al., 2010). Further experiments will be necessary to determine whether this is also the case in chromaffin cells and whether the different kinetic components display different Ca2+ sensitivity.
The central finding of the present study is that the Ca2+ dependence of fission-pore kinetics requires Syt1. In the absence of Syt1, fission-pore dynamics is Ca2+ independent. The complete inhibition of endocytosis by the KR peptide at 100 μm concentration (Fig. 4A) indicates that endocytosis is mediated via an AP-2–Syt interaction. Similarly, a peptide derived from synaptotagmin 2 (Syt2) blocks CME in the calyx of Held where Syt2 is the predominant synaptotagmin isoform (Hosoi et al., 2009). Like Syt1 (Zhang et al., 1994), all synaptotagmin isoforms, including Syt4, bind AP-2 (Ullrich et al., 1994). In contrast to Syt1, the C2 domain of Syt4 does not display Ca2+ binding activity (Ullrich et al., 1994; Li et al., 1995; Dai et al., 2004). It is therefore possible that the endocytic events with Ca2+-independent fission-pore dynamics in Syt1 KO cells are mediated by Syt4. Interestingly, a recent study showed that overexpression of Syt4 increased the endocytic fission-pore duration in PC12 cells (Zhang and Jackson, 2010), consistent with our result that at physiological [Ca2+]e, the fission-pore duration is increased in Syt1 KO cells (Fig. 3G,H).
It has been proposed that Syt4 may play a role in regulating neurotransmitter release (Moore-Dotson et al., 2010), fusion pore dynamics during exocytosis (Wang et al., 2001, 2003), and granule maturation (Ahras et al., 2006) in PC12 cells, a chromaffin cell-derived cell line. However, in a recent immunocytochemical analysis confirming that Syt4 is also expressed in primary rat chromaffin cells, it was found that Syt4 was not present in dense core vesicles (Matsuoka et al., 2011). Our results suggest that, in the absence of Syt1, Syt4 may support endocytosis.
Although nuclear magnetic resonance studies have indicated that purified Syt1 has relatively low intrinsic Ca2+ affinity (Fernandez et al., 2001), recent evidence suggests that, when anchored to a membrane with physiological lipid composition, Syt1 displays Ca2+-dependent enhancement in exocytosis even at submicromolar Ca2+ levels (Lee et al., 2010). Such Ca2+ levels are achievable in most nerve terminals following a single action potential or low-frequency stimulation, suggesting that Syt1 may serve as the Ca2+ sensor in endocytosis as well. Previous studies have noted that the rate of synaptic vesicle endocytosis is slower in Syt1 KO animals (Poskanzer et al., 2003, 2006; Nicholson-Tomishima and Ryan, 2004) but an elevation in [Ca2+]e rescues the endocytic defect caused by a Syt1 mutant with low Ca2+ affinity (Poskanzer et al., 2006). However, due to the evidence that there exist multiple forms of endocytosis, such as CME, kiss-and-run, and bulk endocytosis in synapses (Wu et al., 2007), and that the contribution of each form of endocytosis in synaptic vesicle endocytosis varies markedly in different conditions such as when Ca2+ level is changed (Wu et al., 2009), the mechanism for the defect in synaptic vesicle endocytosis from Syt1 KO animals (Poskanzer et al., 2003, 2006; Nicholson-Tomishima and Ryan, 2004) remains uncertain. Our results identify that, in contrast to WT cells, the Ca2+ dependence of fission dynamics during CME is abolished in Syt1 KO cells (Fig. 3H), suggesting that Syt1 is a necessary component for the Ca2+ dependence of CME.
Footnotes
This work was supported by the University of Illinois at Chicago startup fund, Brain Research Foundation Grant BRF SF 2010-06, and National Science Foundation Award 1145581 to L.-W.G. We thank Dr. J. Morgan (University of Texas at Austin) for critical reading of this manuscript.
- Correspondence should be addressed to Liang-Wei Gong, Department of Biological Sciences, University of Illinois at Chicago, 840 West Taylor Street, Chicago, IL 60607. lwgong{at}uic.edu
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