Figure 3. The number of Gal-1-expressing neurons decreases after epileptic seizures. A, Gal-1 immunoreactive cells are almost exclusively neurons. Coimmunostaining in the hippocampus and the cerebral cortex with antibodies against Gal-1 and NeuN. B, The proportion of Gal-1-positive cells identified as neurons is plotted as the percentage of Gal-1-immunoreactive cells also positive for NeuN immunostaining. C, The pattern of neuronal degeneration triggered by pilocarpine injection parallels the pattern of Gal-1 expression in basal conditions. D, In the parietal cortex, the number of neurons expressing Gal-1 decreases 24 and 72 h after pilocarpine-induced seizures. E, Quantification of the total number of Gal-1/NeuN double-positive neurons in the piriform cortex and hippocampus, per 30 μm section. F, p75NTR immunoreactivity in the hippocampus and piriform cortex 24 h after pilocarpine-induced seizures. Scale bars: A, C, F, 200 μm; D, 100 μm. n.s., not statistically significant.