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Articles, Behavioral/Systems/Cognitive

Suppression of β1-Integrin in Gonadotropin-Releasing Hormone Cells Disrupts Migration and Axonal Extension Resulting in Severe Reproductive Alterations

Jyoti Parkash, Irene Cimino, Nicoletta Ferraris, Filippo Casoni, Susan Wray, Hélène Cappy, Vincent Prevot and Paolo Giacobini
Journal of Neuroscience 21 November 2012, 32 (47) 16992-17002; DOI: https://doi.org/10.1523/JNEUROSCI.3057-12.2012
Jyoti Parkash
1Inserm U837, Jean-Pierre Aubert Research Center, Development and Plasticity of the Postnatal Brain and
2UDSL, School of Medicine, Place de Verdun, F-59045, Lille Cedex, France,
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Irene Cimino
1Inserm U837, Jean-Pierre Aubert Research Center, Development and Plasticity of the Postnatal Brain and
2UDSL, School of Medicine, Place de Verdun, F-59045, Lille Cedex, France,
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Nicoletta Ferraris
3Department Human and Animal Biology, University of Turin, Turin, 10123 Italy, and
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Filippo Casoni
1Inserm U837, Jean-Pierre Aubert Research Center, Development and Plasticity of the Postnatal Brain and
2UDSL, School of Medicine, Place de Verdun, F-59045, Lille Cedex, France,
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Susan Wray
4Cellular and Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke–National Institutes of Health, Bethesda, Maryland 20892
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Hélène Cappy
1Inserm U837, Jean-Pierre Aubert Research Center, Development and Plasticity of the Postnatal Brain and
2UDSL, School of Medicine, Place de Verdun, F-59045, Lille Cedex, France,
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Vincent Prevot
1Inserm U837, Jean-Pierre Aubert Research Center, Development and Plasticity of the Postnatal Brain and
2UDSL, School of Medicine, Place de Verdun, F-59045, Lille Cedex, France,
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Paolo Giacobini
1Inserm U837, Jean-Pierre Aubert Research Center, Development and Plasticity of the Postnatal Brain and
2UDSL, School of Medicine, Place de Verdun, F-59045, Lille Cedex, France,
3Department Human and Animal Biology, University of Turin, Turin, 10123 Italy, and
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Abstract

Reproduction in mammals is dependent on the function of hypothalamic neurons whose axons project to the hypothalamic median eminence (ME) where they release gonadotropin-releasing hormone (GnRH) into a specialized capillary network for delivery to the anterior pituitary. These neurons originate prenatally in the nasal placode and migrate into the forebrain along the olfactory-vomeronasal nerves. The complex developmental events leading to the correct establishment of the GnRH system are tightly regulated by the specific spatiotemporal expression patterns of guidance cues and extracellular matrix molecules, the functions of which, in part, are mediated by their binding to β1-subunit-containing integrins. To determine the biological role of these cell-surface proteins in reproduction, Cre/LoxP technology was used to generate GnRH neuron-specific β1-integrin conditional KO (GnRH-Itgb1−/−) mice. Loss of β1-integrin signaling impaired migration of GnRH neurons, their axonal extension to the ME, timing of pubertal onset, and fertility in these mice. These results identify β1-integrin as a gene involved in normal development of the GnRH system and demonstrate a fundamental role for this protein in acquisition of normal reproductive competence in female mice.

Introduction

Reproductive competence in mammals is centrally regulated through the hypothalamic—pituitary–gonadal axis and depends on gonadotropin-releasing hormone (GnRH) secretion (Wray, 2010). These neurons project to the median eminence (ME) of the hypothalamus where GnRH is released into the pituitary portal blood for delivery to the anterior pituitary, eliciting the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (Wray, 2010). GnRH-secreting neurons originate in the nasal placode during embryonic development and migrate to the hypothalamus apposed to olfactory-vomeronasal nerves (Schwanzel-Fukuda and Pfaff, 1989; Wray et al., 1989).

During the last 20 years, many molecular pathways that guide GnRH migration have been identified. However, only a few factors, including fibroblast growth factor-2 (Gibson et al., 2000; Tsai et al., 2005; Gill and Tsai, 2006), brain-derived neurotrophic factor (Cronin et al., 2004), and kisspeptin (Fiorini and Jasoni, 2010) are known to affect GnRH axon or neurite growth in vitro and/or in vivo. Secreted signaling molecules as well as extracellular matrix (ECM) proteins control the activity of cell-surface receptors that regulate the interactions of GnRH cells with each other, with the olfactory axonal scaffold, and with environmental cues (Wray, 2010). The integrins are a family of heterodimeric transmembrane receptors consisting of an α- and β-subunit, which mediate interactions between cells as well as between cells and the ECM (Reichardt and Tomaselli, 1991; Hynes, 2002). During brain development, integrins are involved in migration, axonal guidance, synaptogenesis, and peripheral nerve regeneration (Pasterkamp et al., 2003; Brakebusch and Fässler, 2005; Gardiner et al., 2005, 2007; Cingolani and Goda, 2008; Plantman et al., 2008; Moser et al., 2009).

The β1-subunit-containing integrins (β1-integrin) represent the largest subgroup of integrins (Brakebusch and Fässler, 2005). GnRH neurons express β1-integrin throughout their development and Semaphorin 7A signals through this cell-surface protein to regulate GnRH cell migration (Messina et al., 2011). Null mutation of the β1-integrin gene in vivo has been achieved in mice and results in embryonic lethality (Fässler and Meyer, 1995; Stephens et al., 1995). The experiments in this paper study the effect of β1-integrin on development of the GnRH system and reproduction by generating mice lacking this gene in GnRH neurons using the Cre/LoxP binary recombination system (Hamilton and Abremski, 1984; Singh et al., 2009). The absence of β1-integrin altered migration of GnRH neurons and targeting of their axons to the ME, resulting in suboptimal maturation of the GnRH system in the adult brain. Examination of fertility revealed that lack of β1-integrin in GnRH neurons caused delayed pubertal onset and disruption of estrous cyclicity in female GnRH-Itgb1−/− mice, greatly impairing reproduction. These results demonstrate a critical role for β1-integrin in GnRH neuronal function and mammalian reproduction.

Materials and Methods

Generation of GnRH neuron-specific β1-integrin (Itgb1) conditional KO mice.

GnRH::Cre mice (Yoon et al., 2005) were kindly provided by Dr. Catherine Dulac (Howard Hughes Medical Institute, Cambridge MA). Itgb1loxP/loxP mice, in which exon 3 of β1-integrin is flanked by loxP sites, were purchased from The Jackson Laboratory, maintained on a controlled 12 h dark/light cycle, provided with food and water ad libitum, and genotyped as described earlier (Stephens et al., 1995). The GnRH::Cre; Itgb1loxP/loxP mice, designated as GnRH-Itgb1−/− mice, were generated by first mating female GnRH::Cre with male Itgb1loxP/loxP mice and then crossing a heterozygous (GnRH::Cre; Itgb1loxP/+) female with a heterozygous male to generate six genotype combinations. GnRH::Cre; Itgb1loxP/loxP mice represent the homozygous conditional KO mice. GnRH::Cre; Itgb1+/+ (GnRH-Itgb1+/+) littermates were used as controls for all studies. All the transgenic lines were bred in our laboratory on a C57BL/6J;129sv background.

Generation of GnRH::Cre;Itgb1loxP/loxP; GnRH::GFP triple transgenic mice.

GnRH::GFP mice (Spergel et al., 1999) were kindly provided by Dr. Daniel J. Spergel (Section of Endocrinology, Department of Medicine, University of Chicago, Illinois). GnRH-Itgb1−/− mice were first crossed with GnRH::GFP animals to obtain triple heterozygous transgenic mice. Then a heterozygous (GnRH::Cre;Itgb1loxP/+; GnRH::GFP) female was crossed with a heterozygous male to generate all genotype combinations.

Genotyping and DNA extraction.

For genotyping, three pairs of primers were used: GnRH-Cre-specific primers, sense 5′-CTGGTG TAGCTGATGATCCG-3′, and antisense 5′-ATG GCT AAT CGC CAT CTT CC-3′; and Itgb1loxP/loxP primers, sense 5′-CGGCTCAAAGCAGAGTGTCAGTC-3′, and antisense 5′-CCACAACTTTCCCAG TTA GCT CTC-3′. GnRH-GFP mice were selected by PCR analysis of mouse tail DNA with primer GnRH 51 5′-GAAGTACTCAACCTACCAACGGAAG-3′ and antisense primer hGFP1 5′-GCCATCCAGTTCCACGAGAATTGG-3′, which amplified a 278 bp DNA fragment in mice transgenic for the GnRH-GFP minigene.

To obtain the genomic DNA of pups, a clipping from the ear or tail was collected and placed in 10% Chelex-100 resin (Bio-Rad) with 0.1%-Tween 20 and 0.15 mg/ml proteinase K. Samples were incubated at 50°C for 90 min, proteinase K was inactivated at 95°C for 20 min, and the solution was cooled to 10°C. PCRs were performed using 7 μl of DNA.

PCR was performed using a thermocycler (35 cycles: 30 s denaturation at 94°C, 1 min annealing at 58–65°C, and 2 min elongation at 72°C).

Animals.

Mice were housed in a room with controlled photoperiod (12 h light/dark) and temperature (21–23°C) with food and water ad libitum, in the animal facility of the Jean-Pierre Aubert Research Center at the Lille 2 University School of Medicine, France. All experiments were performed in accordance with the guidelines for animal use specified by the European Communities Council Directive of November 24, 1986 (86/609/EEC), regarding mammalian research and in accordance with National Institutes of Health (NIH)–National Institute of Neurological Disorders and Stroke guidelines, and Institutional Animal Care and Use Committee approval.

For immunohistochemical analysis, embryos were obtained from timed-pregnant control or conditional β1-integrin KO mice and anesthetized with an intraperitoneal injection of chloral hydrate (8%; 350 mg/kg). Heads from the embryos were washed thoroughly in cold 0.1 m PBS, fixed in fixative solution [4% paraformaldehyde (PFA), 0.2% picric acid in 0.1 m PBS, pH 7.4] for 6–8 h at 4°C and cryoprotected in 20% sucrose overnight at 4°C. The following day, heads were embedded in OCT embedding medium (Tissue-Tek), frozen on dry ice, and stored at −80°C until sectioning. P7 pups and adult mice (3–5 months old) were anesthetized with chloral hydrate (400 mg/kg, i.p.) and perfused transcardially with 10 ml of saline, followed by 100 ml of 4% PFA, pH7.4. Brains were removed and immersed in the same fixative for 2 h at 4°C and stored in 0.1 m PBS until slicing. Free-floating coronal sections (35 μm thick) were cut on a vibratome (VT1000S; Leica) and processed for immunohistochemistry.

Fluorescence-activated cell-sorter analysis.

Heterozygous GnRH::Cre; Itgb1loxP/+; GnRH::GFP mutants were crossed to obtain in the same litter GnRH::Cre; Itgb1+/+; GnRH::GFP, GnRH::Cre; Itgb1loxP/+; GnRH::GFP and GnRH::Cre; Itgb1loxP/loxP; GnRH::GFP. Embryos were harvested at E12.5 from timed-pregnant GnRH::Cre;Itgb1loxP/+; GnRH::GFP mice, previously anesthetized with an intraperitoneal injection of chloral hydrate (8%; 350 mg/kg) and killed by cervical dislocation. Nasal regions were dissected from each embryo and dissociated using a papain-based dissociation protocol previously described (Maric et al., 2003). After dissociation, the cells were physically purified using an EPICS ALTRA flow cytometer (Beckman Coulter). Sorted GFP-positive cells (yield: 600–800 cells obtained from each E12.5 embryo of the litter) were collected into a tube containing 500 μl of sterile HBSS (Invitrogen) and subsequently centrifuged for 1 min at 7500 g (maximum) to relocate material to the bottom of the tube. HBSS was then aspired and 8 μl of a solution containing 1 μl of 0.1% Triton X-100 and 7 μl of Prime RNase inhibitor (diluted 1:100 in diethylpyrocarbonate-treated water; Invitrogen) was added. Captured cells were used to synthesize first-strand cDNA using the SuperScript III First-Strand Synthesis System for reverse transcription (RT)-PCR (Invitrogen) following the manufacturer's instructions. Controls without reverse transcriptase were performed to demonstrate the absence of contaminating genomic DNA. RNAs isolated from heads of E12.5 embryos and GT1–7 cells were also reverse transcribed and used as positive controls. Total RNA from GT1–7 cells and E12.5 heads was isolated by extraction with TRIzol (Invitrogen).

The genotype of embryos was verified after fluorescence-activated cell sorting (FACS) isolation and cDNAs of GnRH-GFP neurons isolated either from GnRH::Cre;Itgb1+/+;GnRH::GFP or GnRH::Cre;Itgb1loxP/loxP; GnRH::GFP embryos (n = 5 for each genotype) were used for the PCR analysis. PCR was performed for GnRH and Itgb1 at 35 cycles on a thermocycler (30 s denaturation at 94°C, 30 s annealing at 55–65°C, and 2 min elongation at 72°C). PCR primer pairs were as follows: GnRH forward primer, 5′-GCTAGGCAGACAGAAACTTGC-3′; GnRH reverse primer, 5′-GCATCTACATCTTCTTCTGCC-3′; L19 (ribosomal housekeeping gene) forward primer, 5′-CCTGAAGGTCAAAGGGAATGTGTTC-3′ and reverse primer, 5′-GGACAGAGTCTTGATGATCTCCTCC-3′; Itgb3 forward primer, 5′-AGGCTTGTGGTGCTTGGGCG-3′; and Itgb3 reverse primer, 5′-GGAGGTGGGGAGGGTGTCAGG-3′. Itgb1 primers were designed in exon 3 of β1-integrin gene, which is floxed in conditional mutant mice when Cre recombination takes place. PCR primer pairs were as follows: Itgb1 forward primer, 5′-GCAGGGCCAAATTGTGGGTGGT-3′ and Itgb1 reverse primer, 5′-GGCCGGAGCTTCTCTGCCAT-3′.

Ovarian histology and quantitative analysis.

Ovaries were collected from 3-month-old control and conditional KO mice, fixed in 4% PFA solution and stored at 4°C. Paraffin-embedded ovaries were sectioned at a thickness of 5 μm (histology facility, University of Lille 2, France) and stained using hematoxylin-eosin protocol. The number of corpora lutea, Graafian follicles, and atretic follicles were counted on photomicrographs from every tenth section throughout the ovary and statistical analysis was performed as described below.

Estrous cyclicity.

To examine the possible effects of mutations on estrous cyclicity, vaginal lavage of female GnRH::Cre; Itgb1loxP/loxP mice and their control littermates (GnRH::Cre; Itgb1+/+) was performed every day (10:00 A.M. to 1:00 P.M.) using 0.9% saline. Smears were observed under the microscope and the phase identified as diestrus (M/D) if they predominantly contained leukocytes, as proestrus (P) if they predominantly contained basal and cornified nucleated cells, and as estrous (E) if they predominantly contained cornified epithelial cells. An estrous cycle was considered normal when the vaginal lavage had leukocytes for 2 d followed by 1 d of nucleated and 1–2 d of cornified cells.

Gonadal steroid-induced LH surge protocol in ovariectomized animals.

Mice were bilaterally ovariectomized (OVX) and implanted subcutaneously with SILASTIC capsules containing 17β-estradiol (E2; 1 μg/20 g body weight). SILASTIC capsules were prepared as follows: crystalline E2 was dissolved in absolute ethanol, mixed with SILASTIC medical adhesive (Type A) (Dow Corning) at a concentration of 0.1 mg/ml adhesive, and injected into SILASTIC tubing (Dow Corning; internal diameter, 1 mm; external diameter, 2.125 mm) (Bronson, 1981; Clarkson et al., 2008). Six days after OVX, mice received a single subcutaneous injection of 17β-estradiol 3-benzoate (1 μg/20 g of body weight in sesame oil) at 9:00 A.M. On the following day, animals received another subcutaneous injection of progesterone (500 μg/20 g body weight in sesame oil) at 9:00 A.M. Between 7:30 and 8:30 P.M. (lights off at 8:00 P.M.) on the same day, mice were anesthetized with an overdose of chloral hydrate (400 mg/kg, i.p.) and trunk blood was collected for LH assay. Trunk blood was collected in tubes containing EDTA (0.2 m) and centrifuged at 6500 rpm for 15 min at 4°C and the supernatant-obtained (plasma) was stored at −80°C until ELISA for LH.

LH assay.

Plasma LH was measured using Rodent LH ELISA kit (ERKR7010-A; Endocrine Technologies) with a sensitivity of 0.3 ng/ml and 7% intra-assay and 10% interassay coefficients of variation.

Immunohistochemistry.

Tissues were cryosectioned (Leica cryostat) at 16 μm for embryos, and at 35 μm for free-floating sections for adult brains. Immunohistochemistry was performed as previously reported (Giacobini et al., 2008), using Alexa-Fluor 488-conjugated (1:400) and Cy3-conjugated (1:800) secondary antibodies (Invitrogen). Fluorescent specimens were mounted using 1,4-diazabicyclo[2.2.2]octane (Sigma-Aldrich). The primary antisera used were as follows: rabbit anti-GnRH (1:3000), a generous gift from Prof. G. Tramu (Centre Nationale de la Recherche Scientifique, URA 339, Université Bordeaux I, Talence, France) (Beauvillain and Tramu, 1980) and rat anti-β1-integrin (1: 500; BD 558741).

Image analysis.

Images were captured using a Nikon microscope (Eclipse 80i) and 2×/0.06 NA, 10×/0.30 NA, and 20×/0.50 NA objectives (Nikon) equipped with a digital camera (CX 9000; MBF Bioscience). For observation coupled with confocal analysis, a laser-scanning Fluoview confocal system (IX70; Olympus) and 10×/0.30 NA, 20×/0.70 NA, and 60×/1.25 NA objectives (Olympus) were used. Subsequent analysis of digitized images was performed with ImageJ (NIH, Bethesda, Maryland; http://imagej.nih.gov/ij/) and Photoshop (Adobe) software to process, adjust, and merge the photomontages.

Analysis of GnRH neurons in transgenic mice.

Serial sagittal sections (16 μm) from E14.5 GnRH-Itgb1+/+ (n = 4) and GnRH-Itgb1−/− (n = 7) mice were cut and immunolabeled for GnRH throughout the head. Quantitative analysis of GnRH neuronal number, as a function of location, was performed over three regions (the nasal compartment, the nasal/forebrain junction, and ventral forebrain). Serial coronal sections (35 μm) through the organum vasculosum of the lamina terminalis (OVLT) and ME of adult GnRH-Itgb1+/+ (females, n = 5; males, n = 3) and GnRH-Itgb1−/− (females, n = 5; males, n = 4) mouse brains were labeled for GnRH. Total number of GnRH cells was calculated for each brain (throughout the entire brain) and combined to give group means ± SEM. No ectopic localization of GnRH neurons was detected in the brains of mutant mice, both during embryonic development as well as at the adult stage, compared with control animals, suggesting that the GnRH neurons were not misrouted.

The density of GnRH-immunoreactive terminals in the ME of control and GnRH-Itgb1−/− mice was also evaluated. Two medial sections per animal (n = 3 each genotype) were chosen. Fiber intensity was evaluated with a confocal microscopy, Zeiss LSM 710. Images (24-bit) were collected with set parameters at 1.5 μm intervals through a 35 μm section using a 10× objective. Maximum intensity projections were calculated with ZEN 2009 (Carl Zeiss) algorithms for each series of confocal images that were binarized with ImageJ software (NIH; http://imagej.nih.gov/ij/). A user-defined threshold parameter was used and applied to each section to optimize detection of labeled fibers. The same threshold values were applied to all images to avoid subjective evaluation. The density values of labeled fibers were calculated based on the number of total pixels in each binarized image per area and combined to give group means ± SEM.

Nasal explants.

Embryos were obtained from timed-pregnant animals. Nasal pits of E11.5 NIH Swiss mice were isolated under aseptic conditions in Gey's Balanced Salt Solution (Invitrogen) enriched with glucose (Sigma-Aldrich) and maintained at 4°C until plating. Explants were placed onto glass coverslips coated with 10 μl of chicken plasma (Cocalico Biologicals). Thrombin (10 μl; Sigma-Aldrich) was then added to adhere (thrombin/plasma clot) the explant to the coverslip. Explants were maintained in defined serum-free medium (SFM) (Fueshko and Wray, 1994) containing 2.5 mg/ml Fungizone (Sigma-Aldrich) at 37°C with 5% CO2 for up to 30 d in vitro (DIV). From culture day 3–6, fresh medium containing fluorodeoxyuridine (8 × 10−5 m; Sigma-Aldrich) was provided to inhibit the proliferation of dividing olfactory neurons and non-neuronal explant tissue. The medium was replaced with fresh SFM twice a week.

Functional assays in nasal explants.

To determine the function of β-integrin on GnRH cell migration and axon outgrowth, pharmacological perturbation was performed on explants using Echistatin. Echistatin is a 49 aa protein with an Arg-Lys-Asp (RGD) sequence that is a member of the disintegrin family that occurs in the venom of Echis carinatus. It specifically inhibits β1- and β3-integrins (Pfaff et al., 1994). Explants in experimental groups were maintained in SFM or SFM plus Echistatin (0.1 μm) at 3 DIV for 72 h. Drug concentrations were based on data from previous studies (Pasterkamp et al., 2003). Control explant medium was changed, as in the treatment group, at 3 and 6 DIV. At 7 DIV, explants were processed for immunocytochemistry for GnRH and density of the fibers in the periphery of the explants quantified. The main tissue mass contained the nasal pit/olfactory epithelial region, surrounding mesenchyme, and nasal midline cartilage. The periphery refers to the area surrounding the main tissue mass into which cells had spread and/or migrated.

Quantification of GnRH fiber density was performed on digitized photomicrographs (using a 20× UPlanFl Phase objective; Olympus I X50 inverted microscope equipped with a CCD CoolSNAP-Pro camera; Media Cybernetics). First, a threshold was manually set to specifically demonstrate the network structures in the image. The quality and resolution of the images allowed reliable and exclusive threshold of the networks without the need for image filtering. Images were then placed in bins and subjected to the “Skeletonize” function of ImageJ software, which excluded the GnRH cell somas from the analysis (see Fig. 4d,f). The corresponding density was measured by dividing the mean pixel area of GnRH fibers for the total area occupied by the fiber network. This quantitative method was chosen because the complex nature of the fiber network prevented the quantification of individual fiber length and branching. Three pictures were taken for each explant and average density was calculated for each sample to homogenize internal variability. Finally, mean density among treatment groups was calculated. Twelve animals were used for the control group (n = 12) and 7 for the treatment group (Echistatin; n = 7).

The data are presented as means ± SEM. All experiments used explants generated from different individuals on multiple culture dates.

Statistical analysis.

For comparison of multiple groups, statistical significance was determined using a one-way ANOVA (for Gaussian distributed data) followed by Fisher 's least significant difference post hoc analysis test. For comparison between two groups, a two-tailed unpaired Student's t test was used. The significance level was set at p < 0.05. Data groups are indicated as mean ± SEM.

Results

Generation of conditional KO mice lacking β1-integrin in GnRH neurons

Recently it was shown that GnRH neurons begin expressing β1-integrin at early stages of embryonic development and that Semaphorin 7A regulates the migration of immortalized GnRH cells through β1-integrin activation (Messina et al., 2011). Interestingly, in the same work it has been shown that GnRH neurons expressed β1-integrin at comparable levels in the migratory and postmigratory stages, supporting the notion that this molecule might regulate several biological processes throughout development.

In the present work, the gene encoding the β1-integrin subunit was inactivated in GnRH neurons by crossing floxed β1-integrin (Itgb1) mice with LoxP sites flanking exon 3 (Jax mice; Fig. 1a) with GnRH::Cre mice (Yoon et al., 2005), which express the Cre recombinase gene in GnRH neurons, to obtain GnRH::Cre; Itgb1+/+ (GnRH-Itgb1+/+) and GnRH::Cre; Itgb1loxP/loxP (GnRH-Itgb1−/−) mice. Crosses typically displayed Mendelian segregation of the three embryonic genotypes: GnRH::Cre; Itgb1loxP/loxP animals, which were homozygous null for the Itgb1 gene in GnRH cells and wild type for this gene in other cell types; GnRH::Cre; Itgb1loxP/+ animals, which were heterozygous for the Itgb1 gene in GnRH cells; and GnRH::Cre; Itgb1+/+ which were wild type for Itgb1 in all cells, including GnRH cells. To confirm cell-specific deletion of Itgb1 in GnRH cells, GnRH::Cre; Itgb1loxP/loxP animals were crossed with GnRH::GFP animals, which express the green fluorescent protein (GFP) under the control of the GnRH promoter (Spergel et al., 1999) so that reverse transcription (RT)-PCR analysis could be performed on embryonic GnRH-GFP cells isolated through fluorescence-activated cell sorting (FACS). E12.5 embryos were harvested from pregnant triple-mutant mice. At this stage, the majority of the GnRH population is located within the nasal region (Fig. 1b, green dots) (Schwanzel-Fukuda and Pfaff, 1989; Wray et al., 1989). Therefore, noses were dissected from mutant GnRH::Cre; Itgb1loxP/loxP; GnRH::GFP (GnRH-Itgb1−/−;GnRH-GFP) and control GnRH::Cre; Itgb1+/+;GnRH::GFP E12.5 embryos (GnRH-Itgb1+/+; GnRH-GFP) and purified GnRH-GFP-positive neurons were isolated by FACS (Fig. 1b). RT-PCR experiments were performed on cDNAs of primary GnRH-GFP neurons, positive controls (GT1–7 cells and E11.5 heads), and negative control cDNAs (water and –RT). Transcripts of the expected molecular size for mouse GnRH and the housekeeping gene L19 were found in all samples but negative control (Fig. 1c). Primers within the third exon of the Itgb1 gene, which is floxed upon Cre recombination, were used. RT-PCR analysis revealed expression of Itgb1 in the positive samples and in the GnRH-Itgb1+/+; GnRH-GFP cells, whereas Itgb1 was lacking in GnRH-Itgb1−/−; GnRH-GFP neurons (Fig. 1c), confirming cell-specific deletion of Itgb1 in GnRH neurons in conditional-KO mice.

Figure 1.
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Figure 1.

Generation of GnRH neuron-specific Itgb1 KO mouse. a, Exon 3 of the β1-integrin gene (Itgb1) is flanked by two LoxP sites; Cre recombinase expression is regulated by the mouse GnRH promoter. GnRH::Cre; Itgb1loxP/loxP mice were crossed with GnRH::GFP animals, which express the GFP under the control of the GnRH promoter, to generate triple-transgenic mice. b, Schematic summarizing the steps of GnRH-GFP cells' isolation. E12.5 embryos were harvested from GnRH::Cre; Itgb1loxP/+; GnRH::GFP pregnant mice; the nasal regions were dissected and GnRH-GFP cells were purified by FACS. c, RT-PCR for GnRH and Itgb1 was performed on total RNA isolated from the indicated samples. Positive (GT1–7 cells; E11.5 whole heads) and negative controls (water) were included in the reaction mix.

Lack of β1-integrin expression in GnRH neurons leads to defects in their migratory process and their axonal targeting to the target tissues

To investigate whether the absence of β1-integrin signaling in GnRH neurons affects their development and migration, the number and distribution of GnRH neurons were examined at E14.5 in control (GnRH-Itgb1+/+) and GnRH-Itgb1−/− mice (Fig. 2a–d). The number of GnRH cells in the nasal compartment (cells located in the olfactory-vomeronasal epithelia and across the nasal mesenchyme), olfactory bulb (ob), and ventral forebrain (vfb) areas of embryos was determined (Fig. 2e, top, red boxes). GnRH-Itgb1−/− animals showed a significant accumulation of GnRH cells in the nasal compartment compared with control littermates (Fig. 2e, bottom). Concomitantly, in mutant mice fewer GnRH neurons were located in the final brain target area (vfb; Fig. 2c–e), consistent with a migratory defect (one-way ANOVA, F(7,43) = 44.3, p < 0.0001). To assess whether this defect was compensated after birth, the number and distribution of GnRH neurons were analyzed in brains of postnatal day 7 (P7) mice. Indeed, the total number of these neuroendocrine cells as well as their localization within the brain was unchanged between GnRH-Itgb1−/− and control infantile animals (GnRH-Itgb+/+ mice, n = 6, mean GnRH cell number = 873 ± 31; GnRH-Itgb−/− mice, n = 4, mean GnRH cell number = 829 ± 52; unpaired Student's t test, t(8) = −0.768, p = 0.46).

Figure 2.
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Figure 2.

GnRH-Itgb1−/− mice show defective GnRH migration during embryonic development. a–d, Confocal photomicrographs showing GnRH immunoreactivity in sagittal sections of E14.5 control (a, c) and GnRH-Itgb1−/− (b, d) embryo heads. Dashed lines indicate the boundary between the nose and the forebrain. Analysis of the location of GnRH neurons in three regions along the migratory pathway, nasal compartment (nose), olfactory bulb (ob), and ventral forebrain (vfb) reveals a significant accumulation of cells in the nasal region of GnRH-Itgb1−/− mice when compared with control mice (a, b). Consistently, fewer GnRH neurons are located in the vfb of GnRH-Itgb1−/− than of GnRH-Itgb1+/+ embryos (c vs d). e, Top, Schematic of a sagittal section of an E14.5 embryo. Red boxes indicate the areas of the analysis of GnRH cell distribution. oe, Olfactory epithelium; vno, vomeronasal organ. Bottom, Quantitative analysis revealed a migratory defect of GnRH neurons at this developmental stage. Data are represented as means ± SEM (n, number of embryos; **p < 0.005, *p < 0.05, Fisher 's least significant difference post hoc analysis). Scale bars: (in a) a, b, 20 μm; (in c) c, d, 40 μm.

However, in adult animals, a significant 30% reduction was found in the number of GnRH cell bodies (Fig. 3a,b) of GnRH-Itgb−/− mice compared with control littermates, regardless of the sex (GnRH-Itgb+/+, n = 8, mean GnRH cell number = 712 ± 33; GnRH-Itgb−/−, n = 9, mean GnRH cell number = 436 ± 14. n, number of mice; unpaired Student's t test, t(15) = 7.98, p < 0.0001). No differences in the number and bilateral distribution of GnRH neurons was observed between male and female brains of the same genotype (GnRH-Itgb+/+ females, n = 5, mean GnRH cell number = 672 ± 44; GnRH-Itgb+/+ males, n = 3, mean GnRH cell number = 780 ± 15; unpaired Student's t test, t(6) = −1.81, p = 0.12; GnRH-Itgb−/− females, n = 5, mean GnRH cell number = 443 ± 26; GnRH-Itgb−/− males, n = 4, mean GnRH cell number = 427 ± 5; unpaired Student's t test, t(7) = 0.52, p = 0.62).

Figure 3.
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Figure 3.

The lack of β1-integrin in GnRH cells leads to a suboptimal number of GnRH neurons and reduced innervation of the median eminence (me) in adulthood. a–d, Confocal photomicrographs showing GnRH immunoreactivity in coronal sections of adult control (a, c) and conditional mutant (b, d) brains. Images show a significant reduction in the number of GnRH neurons at the level of the OVLT (arrowheads). In addition, β1-integrin-KO GnRH neurons also display shorter neurites than control cells (insets in a and b, arrowheads). c, d, The me shows a dramatic loss of GnRH-immunoreactive terminals in GnRH-Itgb1−/− mice (d) when compared with control littermates (c). III V, third ventricle. Scale bars: a and b and insets (in a), 40 and 20 μm, respectively; c and d (in c), 200 μm.

Notably, the GnRH neurons of GnRH-Itgb1−/− animals displayed shorter neurites than those of GnRH-Itgb+/+ mice (Fig. 3a,b, insets). Consistent with this observation, densitometric analysis of immunostaining of the ME, the terminal field of GnRH neuroendocrine neurons, revealed a marked loss of GnRH fibers in homozygous mice when compared with control mice (Fig. 3c,d). The innervation of the ME of female GnRH-Itgb1−/− mice was reduced by >70% when compared with control mice (GnRH-Itgb+/+, n = 3, mean density of fibers = 409.8 ± 97; GnRH-Itgb−/−, n = 3, mean density of fibers = 80.6 ± 25; unpaired Student's t test, t(15) = 3.12, p < 0.005). Quantitative analysis revealed a 33% reduction in the innervation of the ME in males (GnRH-Itgb+/+, n = 3, mean density of fibers = 410 ± 20; GnRH-Itgb−/−, n = 4, mean density of fibers = 271.3 ± 12; unpaired Student's t test, t(5) = 6.43, p < 0.005), thus evidencing a marked sex difference percentage reduction of fiber density in males vs females, p < 0.05).

Inhibition of β1-integrin in vitro disrupts GnRH fibers' network

To determine the role of Itgb1 in regulation of GnRH fiber elongation, a nasal explant model was used (Fig. 4a). Explants were treated with Echistatin (0.1 μm), a selective inhibitor of β1- and β3-integrins (Pfaff et al., 1994). RT-PCR experiments for β3-integrin (Itgb3) were performed on cDNAs of primary E12.5 GnRH-GFP sorted neurons, GT1–7 cells, which display features of mature postmigratory GnRH neurons, and water (Fig. 4b). A transcript of the expected molecular size for the mouse Itgb3 was found in GT1–7 cells but not in primary migratory GnRH cells and in the negative control (Fig. 4b), indicating that Echistatin could only act via Itgb1 signaling in GnRH cells in explants.

Figure 4.
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Figure 4.

β1-integrin inhibition disrupts GnRH fibers network in vitro. a, Schematic of a nasal explant removed from an E11.5 mouse and maintained in serum-free media for 7 DIV. Ovals represent olfactory pit epithelium (OPE); in center is nasal midline cartilage (NMC) and surrounding mesenchyme (M). GnRH neurons (dots) migrate from OPE and follow olfactory axons to the midline and off the explant into the periphery. Boxed region within schematic is area shown in c–f. b, Representative gel of PCR products for GnRH and β3-integrin (Itgb3) from GnRH neurons isolated from GnRH::GFP E12.5 nasal regions through FACS. Positive (GT1–7 cells) and negative controls (water) were included in the reaction mix. c, e, Explants in experimental groups were maintained in SFM (CNTR) with or without Echistatin (0.1 mm) at 3 DIV for 72 h and fixed at 7 DIV for immunocytochemical processing (GnRH, green). d, f, Representative images binarized and subjected to the “Skeletonize” function of ImageJ software. Scale bars: (in c) c–f, 40 μm.

The application of Echistatin severely restricted the GnRH fiber network in the periphery of the explant (Fig. 4c–f). Quantitative analysis revealed a significant reduction in the density of the GnRH-immunoreactive fiber network following β1-integrin blockage (controls, n = 12, mean density of fibers = 0.057 ± 0.005; Echistatin-treated, n = 7, mean density of fibers = 0.042 ± 0.002). Data are combined values from three independent experiments (n, number of explants; unpaired Student's t test, t(17) = 3.06, p < 0.05). The absence of a change in GnRH cell number after Echistatin treatment (control: 197 ± 24, n = 12; Echistatin: 220 ± 29, n = 7; unpaired Student's t test, t(17) = −0.72, p = 0.48) indicates that this drug does not exert mitogenic or survival effects on GnRH neurons in vitro and is consistent with the hypothesis that β1-integrin regulates neurite sprouting of primary GnRH neurons.

GnRH::Cre;Itgb1loxP/loxP female mice exhibit impaired fertility

The observation that GnRH-Itgb1−/− mice had a dramatic loss of GnRH innervation in the ME suggested that fertility could be disrupted. Since alterations in neuroendocrine activity are expected to result in impaired reproductive capacity, fertility was examined in control and conditional Itgb1 KO mice using a continuous mating protocol for 90 d. Male reproductive capacity was assessed by breeding young adult (P90) GnRH-Itgb1−/− males with confirmed control dams (GnRH-Itgb1+/+) and monitoring the occurrence of litters over 3 months. In adult males, all fertility parameters examined, such as (1) number of days required to produce one litter (unpaired Student's t test, t(7) = 0.4, p = 0.7), (2) fertility index (number of litters/month) (unpaired Student's t test, t(13) = 0.26, p = 0.79), and (3) number of pups/litter, did not differ between control and GnRH-Itgb1−/− mice (Fig. 5a–c; unpaired Student's t test, t(56) = 0.63, p = 0.53). In contrast, female GnRH-Itgb1−/− mice showed significant alterations of the reproductive axis. Both the fertility index (one-way ANOVA, F(2,18) = 4.09, p = 0.04) and the number of pups/litter (one-way ANOVA, F(2,66) = 5.94, p = 0.004), were significantly reduced in the GnRH-Itgb1−/− females (Fig. 5a–c). Whereas, the latency to first pregnancy was not affected in any group analyzed (one-way ANOVA, F(2,16) = 0.12, p = 0.9).

Figure 5.
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Figure 5.

Female GnRH-Itgb1−/− mice exhibit impaired fertility. Fertility in GnRH-Itgb1+/+ and GnRH-Itgb1−/− female mice. Matings were performed for 90 d. a, The latency to first pregnancy was not affected in any group analyzed. b, The total number of litters per female was significantly reduced in conditional mutant female mice compared with control females mated with either GnRH-Itgb1−/− or GnRH-Itgb1+/+ males. c, Conditional mutant female mice gave birth to a reduced number of pups per litter compared with control females. Data are represented as means ± SEM (n, number of animals; *p < 0.01, Fisher 's least significant difference post hoc analysis). d, Morphological analysis of ovaries from 3- to 5-month-old control (GnRH-Itgb1+/+; n = 5) and mutant mice (GnRH-Itgb1−/−; n = 5). Ovary sections (5 μm thick) were stained with hematoxylin-eosin. In GnRH-Itgb1−/− females, the ovaries displayed a greater number of atretic follicles (AF) and a relative paucity of corpora lutea (CL), when compared with the ovaries of control littermates in which follicular development was normal. GF, Graafian follicles. e, The numbers of corpora lutea, Graafian follicles, and atretic follicles were quantified in the ovaries of CNTR and KO mice. Data are represented as means ± SEM. ap < 0.05, unpaired Student's t test. Scale bar, 100 μm.

To determine whether the observed defects were associated with an ovulation deficiency, ovarian morphology in 3- to 5-month-old control and GnRH-Itgb1−/− female mice was evaluated. In contrast to control mouse ovaries, which contained large Graafian follicles and several corpora lutea, histological inspection of adult conditional null mouse ovaries revealed a significant reduction in the number of corpora lutea (unpaired Student's t test, t(8) = 3.1, p = 0.01), which serves as a confirmation that there are reduced numbers of ovulations (Fig. 5d,e).

GnRH::Cre;Itgb1loxP/loxP female mice exhibit delayed puberty and abnormal estrous cycles

Since the lack of β1-integrin in GnRH neurons impacted only the reproductive axis in females and not in males, female mice were further characterized. Vaginal opening and first estrus have been shown to be estrogen-dependent processes, which correlate with the onset of puberty in rodents (Ojeda and Skinner, 2006). Thus, weaned GnRH-Itgb1+/+ (n = 12) and GnRH-Itgb1−/− (n = 12) female littermates were examined for vaginal opening and first estrus. Vaginal opening was significantly delayed by ∼6 d in GnRH-Itgb1−/− female mice compared with control females (Fig. 6a; GnRH-Itgb1+/+: 29.1 d ± 0.23, GnRH-Itgb1−/−: 35.4 d ± 0.57; unpaired Student's t test, t(22) = −10.3, p < 0.0001). Analysis of cumulative percentage of vaginal opening within the two groups revealed that whereas, in control females, 100% of the mice had vaginal opening by P28, the entire population of GnRH-Itgb1−/− displayed vaginal opening at P37 (Fig. 6b). Similarly, the first estrus was significantly delayed by ∼10 d in GnRH-Itgb1−/− versus GnRH-Itgb1+/+ mice (Fig. 6a,c; GnRH-Itgb1+/+: 32.5 d ± 0.23, GnRH-Itgb1−/−: 41.9 d ± 0.58; unpaired Student's t test, t(22) = −15, p < 0.0001). Daily inspection of vaginal cytology in GnRH-Itgb1−/− mice revealed absence of normal estrous cyclicity (Fig. 7a). GnRH-Itgb1−/− mice exhibited a persistent diestrus state (GnRH-Itgb1+/+ mean percentage time in proestrus: 14 ± 2, GnRH-Itgb1−/− mean percentage time in proestrus: 1 ± 0.7, unpaired Student's t test, t(22) = 7, p < 0.0001; GnRH-Itgb1+/+ mean percentage time in estrus: 26.5 ± 2, GnRH-Itgb1−/− mean percentage time in diestrus: 10.5 ± 1, unpaired Student's t test, t(22) = 7, p < 0.0001; GnRH-Itgb1+/+ mean percentage time in diestrus: 59 ± 1, GnRH-Itgb1−/− mean percentage time in diestrus: 88 ± 1; unpaired Student's t test, t(22) = −15, p < 0.0001) with the sporadic occurrence of complete 4–5 d ovarian cycles (Fig. 7c), whereas control littermates showed regular estrous cyclicity (Fig. 7a,b). These findings show that GnRH-Itgb1−/− mice display ovarian cyclicity deficits.

Figure 6.
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Figure 6.

Female GnRH-Itgb1−/− mice exhibit delayed puberty and abnormal estrous cyclicity. a, Vaginal opening and time of the first estrus were examined daily from P21 as good indicators of puberty onset in rodents. Vaginal opening in GnRH-Itgb1−/− females was delayed by ∼6 d when compared with control females. Vaginal smears were prepared daily following vaginal opening, and the age at first estrus (defined by the presence of a majority of cornified epithelial cells) was recorded (n = 12 each group). First estrus was delayed by ∼10 d in GnRH-Itgb1−/− females. Data are represented as means ± SEM. n, number of animals; *p < 0.0001, unpaired Student's t test. b, Analysis of cumulative percentage of vaginal opening within the two groups. In control females, 100% of the mice displayed vaginal opening by the P28, whereas the entire population of GnRH-Itgb1−/− mice had vaginal opening by the P37. c, Analysis of cumulative percentage of the first estrus within the two groups. In control females, 100% of the mice had the first estrus by the P34, whereas the entire population of GnRH-Itgb1−/− mice entered puberty at the P44.

Figure 7.
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Figure 7.

Female GnRH-Itgb1−/− mice show abnormal estrous cyclicity. Vaginal cytology was assessed for 20 d in GnRH-Itgb1+/+ (n = 12) and GnRH-Itgb1−/− mice (n = 12). a, Time in each estrous cycle phase as a percentage of the cycle. GnRH-Itgb1−/− females remained in a predominantly diestrus state with the sporadic occurrence of complete ovarian cycles, whereas control littermates showed a regular estrous cyclicity. Data are represented as means ± SEM. n, number of animals; *p < 0.0001, unpaired Student's t test. M/D, metestrus/diestrus; E, estrus; P, proestrus. b, c, Representative estrous cyclicity of control (b) and GnRH-Itgb1−/− mice (c). Control females typically show a regular 4 d ovarian cycle (b), whereas GnRH-Itgb1−/− mice are characterized by with highly irregular cycles (c).

GnRH::Cre;Itgb1loxP/loxP mice do not elevate LH under gonadal steroid-positive feedback condition

In female mice, GnRH-induced LH surge stimulates ovulation, which is an essential process in fertility and is induced by positive feedback of gonadal steroids on the hypothalamic–pituitary axis. To examine the GnRH/LH surge, an OVX-E-P replacement model was used as previously described (Hanchate et al., 2012). While OVX GnRH-Itgb1+/+ mice exhibited an LH surge in response to gonadal steroid treatment (Fig. 8; unpaired Student's t test, t(6) = −198, p < 0.0001), OVX GnRH-Itgb1−/− littermates did not exhibit an LH surge, displaying levels of LH that were near the limit of detection (Fig. 8; unpaired Student's t test, t(8) = 0.67, p = 0.52).

Figure 8.
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Figure 8.

LH surge in GnRH-Itgb1−/−. Profile of LH surge in OVX mice of the two genotypes treated with estrogen and progesterone. Note that while control OVX mice (n = 4) exhibited an LH surge in response to gonadal steroid treatment, GnRH-Itgb1−/− littermates (n = 5) did not, confirming that estrogen-based positive feedback is disrupted. Data are represented as means ± SEM. n, number of animals; *p < 0.0001, unpaired Student's t test.

Discussion

Integrins are heterodimeric (αβ) ECM receptors that are widely expressed throughout the mammalian nervous system (Pinkstaff et al., 1999), where they regulate development and function of neurons (Anton et al., 1999; Benson et al., 2000; Chavis and Westbrook, 2001; Graus-Porta et al., 2001; Huang et al., 2006; Belvindrah et al., 2007; Webb et al., 2007). Using a novel mouse model of β1-integrin ablation in GnRH neurons we uncovered a fundamental role of integrins in the development of the GnRH system, establishment of the hypothalamic–pituitary–gonadal axis and involvement of β1-integrin signaling in the initiation of puberty and regulation of the pre-ovulatory gonadotropin surge.

RT-PCR analysis on GnRH-sorted cells deriving from triple-mutant embryos (GnRH::Cre;Itgb1loxP/loxP; GnRH::GFP) revealed that recombination has already occurred at E12.5, when the majority of this population is migrating across the nasal mesenchyme. Analysis of conditional mutant embryos at E14.5 shows deficits consistent with the reduced migration of GnRH neurons. However, this delay appears to be compensated during later developmental stages with the number and distribution of GnRH neurons being unaffected in the brain of P7 mutants compared with control mice. Indeed, many factors controlling the precise journey of these neuroendocrine cells from nose to brain have been elucidated (Wierman et al., 2011), which could be responsible for such compensatory mechanism.

Interestingly, the number of GnRH cells is significantly reduced by approximately a third in the brains of adult GnRH-Itgb1−/− mice, implying that cell death might occur within this cell population between the infantile and the adult stage. Indeed, it is very well established that integrin-mediated signals are necessary in normal cells to control cell survival and apoptosis (via PI3-kinase and Akt; Hynes, 2002). However, due to the small size of the GnRH neuronal population and the limited temporal window during which apoptosis takes place, it is not possible to determine whether this is the case. The loss of GnRH immunoreactive neurons has also been reported in other mutant mice, such as neuropilin 2−/−, netrin 1−/−, and ephrin 3–5−/− mice, but its cause remains undetermined (Schwarting et al., 2004; Gamble et al., 2005; Cariboni et al., 2007).

At the end of the migratory process, GnRH neurons undergo striking structural plasticity, and elongate their axons to contact the ME. Previous studies have shown that many neurons use members of the integrin family of cell-surface receptors to respond to factors that promote neurite growth and axonal elongation to their final target areas (Hynes, 2002). Consistently, ablation of Itgb1 gene in GnRH neurons leads to defects in neuritogenesis. In fact, GnRH-Itgb1−/− animals display shorter neurites than those of GnRH-Itgb+/+ mice. To verify that such defects were caused by the removal of β1-integrin, perturbation of primary GnRH cells was performed in nasal explants. Indeed, inhibition of β1-integrin significantly disrupted the GnRH fibers network in vitro, further substantiating the notion that integrins are required for the proper neurite outgrowth of these neuroendocrine cells.

The selective deletion of Itgb1 in GnRH neurons is also responsible for striking reproductive defects in female mice, as evidenced by the delay in the onset of puberty, alterations in ovarian morphology, and the reduced number and size of litters. GnRH-Itgb1+/− female mice did not show any reproductive abnormalities (data not shown) indicating that one Itgb1 allele is sufficient to grant normal development of the GnRH axis.

A very interesting aspect of the present study is the finding that male reproductive function was not affected by the disruption of β1-integrin signaling in GnRH neurons, as shown by the continuous mating experiments. Notably, both male and female GnRH-Itgb1−/− mice have a comparable decrease in the size of the GnRH neuronal population within the brain when compared with control mice (30% reduction). However, the extent of reduction in GnRH neuronal projections was sexually dimorphic, being milder in male than in female GnRH-Itgb1−/− mice (33% reduction in male versus 70% reduction in female mutant mice). Hormonal factors, such as sex steroids including estrogens and testosterone, direct formation of sexually dimorphic circuits by influencing axonal guidance and synaptogenesis, neurogenesis, cell migration, cell differentiation, and cell death (Simerly, 2002; Tobet et al., 2009). Several reports indicate that gonadal steroids are capable of modulating the expression of different classes of guidance molecules, which are known to bind β1-integrin to activate their intracellular signaling pathway, in physiological as well as in pathological conditions (Cullinan-Bove and Koos, 1993; Liu et al., 1994; Nikolova et al., 1998; Pavelock et al., 2001; Khan et al., 2005; Nguyen et al., 2011; Richeri et al., 2011). Thus, it is possible that gonadal steroid-dependent mechanisms could be responsible of the sex difference observed in the GnRH neuronal projections to the ME in GnRH-Itgb1−/− mice.

A strong precedent for this notable sex difference does exist since several gene mutations including cyclooxygenase (Lim et al., 1997), progesterone receptor (Lydon et al., 1996), ErbBs (Prevot et al., 2005), and FSH (Kumar et al., 1997) cause female infertility but have no effect on male reproductive capacity.

The phasic secretion of LH from the pituitary gland is perhaps the most significant sex difference in endocrine physiology (Simerly, 1998). In female rats, plasma levels of estradiol increase during the estrous cycle and lead to a massive surge in LH secretion on the afternoon of proestrus (Simerly, 1998). Treatment of OVX adult female rats with exogenous estradiol causes afternoon surges in LH release, yet treatment of gonadectomized male rats fails to cause a similar response due to organizational effect of androgens around the time of birth (Jarzab and Döhler, 1984).

Gonadal steroids via positive feedback to the hypothalamic–pituitary gonadotropic axis control the onset of the LH surge and thus ovulation. After exogenous administration of estradiol benzoate and progesterone to OVX GnRH-Itgb1−/− females, GnRH neurons failed to induce an LH surge, suggesting that the reproductive dysfunction of these animals is due to an attenuated GnRH-system function. We cannot exclude that additional defects affecting the development and function of synapses on GnRH dendrites could occur in the absence of β1-integrin. Recent studies have demonstrated that the dendritic tree of GnRH neurons is subject to marked remodeling during postnatal development (Cottrell et al., 2006) and that the density of spines along GnRH dendrites increases not only during sexual maturation (Cottrell et al., 2006), but also at the onset of the GnRH/LH surge induced by gonadal steroids in OVX adult mice (Chan et al., 2011). Notably, previous works have demonstrated the essential role of integrins in synapse maturation and plasticity (Benson et al., 2000; Chavis and Westbrook, 2001; Huang et al., 2006; Webb et al., 2007; Warren et al., 2012). In addition to these functions, β1-containing integrins have been also shown to influence circuit function by controlling dendritic arbor formation and/or stability. For instance, inhibiting β1-integrin signaling in developing retinal ganglion cells blocks dendritic growth (Lilienbaum et al., 1995), and plating cortical neurons on integrin substrates such as laminin promotes dendritic branching and extension (Moresco et al., 2005).

Thus, the dysfunction of the GnRH system in β1-integrin mutants likely derives from a combination of multiple developmental defects ultimately affecting neuronal activity and secretion of GnRH neurons.

This work demonstrates the critical role of GnRH Itgb1 in the complex developmental signaling pathways that control mammalian reproduction.

Future studies should determine which ligands activating β1-integrin signaling cascade could be modulated by sexual hormones within the CNS and what effect they have on neuronal connectivity and function during critical periods of the GnRH network activation.

Footnotes

  • This work was supported by Compagnia di San Paolo, Turin, Italy (grant number 2008.1224 to P.G.); the Institut National de la Santé et de la Recherche Médicale, Inserm, France (grant number U837); Agence Nationale de la Recherche, ANR, France (grant numbers ANR-2010-JCJC-1404–01 to P.G. and ANR-09-BLAN-0267 to V.P.); the University of Lille 2, Lille, France (Appel a‘ Projets du Conseil Scientifique de l'Universite′ Lille 2 to P.G.); and the Intramural Research Program of the National Institute of Neurological Disorders and Stroke–National Institutes of Health. We thank Meryem Tardivel of the Imaging Core Facility of IFR114 of the University of Lille 2 for technical assistance, Julien Devassine and Delphine Cappe (Animal facility-IFR114) for the maintenance of transgenic mouse lines, the histology facility at University of Lille 2, André Défossez and Claude-Alain Maurage for the histological analyses of specimens, and Nathalie Jouy from the cytometry core facilities (BioImaging Center Lille Nord de France–IFR114).

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to Paolo Giacobini, Inserm U837, Jean-Pierre Aubert Research Center, Unit 837, Development and Plasticity of the Postnatal Brain, Place de Verdun, 59045, Lille Cedex, France. paolo.giacobini{at}inserm.fr

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Journal of Neuroscience
Vol. 32, Issue 47
21 Nov 2012
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Suppression of β1-Integrin in Gonadotropin-Releasing Hormone Cells Disrupts Migration and Axonal Extension Resulting in Severe Reproductive Alterations
Jyoti Parkash, Irene Cimino, Nicoletta Ferraris, Filippo Casoni, Susan Wray, Hélène Cappy, Vincent Prevot, Paolo Giacobini
Journal of Neuroscience 21 November 2012, 32 (47) 16992-17002; DOI: 10.1523/JNEUROSCI.3057-12.2012

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Suppression of β1-Integrin in Gonadotropin-Releasing Hormone Cells Disrupts Migration and Axonal Extension Resulting in Severe Reproductive Alterations
Jyoti Parkash, Irene Cimino, Nicoletta Ferraris, Filippo Casoni, Susan Wray, Hélène Cappy, Vincent Prevot, Paolo Giacobini
Journal of Neuroscience 21 November 2012, 32 (47) 16992-17002; DOI: 10.1523/JNEUROSCI.3057-12.2012
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