Abstract
Several key transcription factors and coregulators important to peripheral nerve myelination have been identified, but the contributions of specific chromatin remodeling complexes to peripheral nerve myelination have not been analyzed. Chromodomain helicase DNA-binding protein 4 (Chd4) is the core catalytic subunit of the nucleosome remodeling and deacetylase (NuRD) chromatin remodeling complex. Previous studies have shown Chd4 interacts with Nab (NGFI-A/Egr-binding) corepressors, which are required for early growth response 2 (Egr2/Krox20), to direct peripheral nerve myelination by Schwann cells. In this study, we examined the developmental importance of the NuRD complex in peripheral nerve myelination through the generation of conditional Chd4 knock-out mice in Schwann cells (Chd4loxP/loxP; P0-cre). Chd4 conditional null mice were found to have delayed myelination, radial sorting defects, hypomyelination, and the persistence of promyelinating Schwann cells. Loss of Chd4 leads to elevated expression of immature Schwann cell genes (Id2, c-Jun, and p75), and sustained expression of the promyelinating Schwann cell gene, Oct6/Scip, without affecting the levels of Egr2/Krox20. Furthermore, Schwann cell proliferation is upregulated in Chd4-null sciatic nerve. In vivo chromatin immunoprecipitation studies reveal recruitment of Chd4 and another NuRD component, Mta2, to genes that are positively and negatively regulated by Egr2 during myelination. Together, these results underscore the necessity of Chd4 function to guide proper terminal differentiation of Schwann cells and implicate the NuRD chromatin remodeling complex as a requisite factor in timely and stable peripheral nerve myelination.
Introduction
In the peripheral nervous system, Schwann cells produce the lipid-rich myelin sheath that envelops axons and provides trophic support vital to nerve development and saltatory propagation of action potentials (Nave and Trapp, 2008). Defects in peripheral myelination underlie one of the most common inherited neurological disorders, Charcot–Marie–Tooth disease (Scherer and Wrabetz, 2008). Maturation of Schwann cells is associated with both activation of a myelin-associated gene network and simultaneous repression of genes that mark the earlier stages of development (Jessen and Mirsky, 2008). One of the major factors regulating myelination is the zinc-finger transcription factor, early growth response-2 (Egr2/Krox20). Analysis of Egr2/Krox20-deficient mice revealed an arrest at the promyelinating stage of Schwann cell development, and it is also required for maintenance of the myelin sheath in adulthood (Topilko et al., 1994; Le et al., 2005a; Decker et al., 2006). Egr2 target genes include several lipid biosynthetic genes and major myelin components, including myelin protein zero (Mpz) and peripheral myelin protein 22 (Pmp22) (Nagarajan et al., 2001). The activity of Egr2 requires interaction with the NGFI-A/Egr binding protein (Nab1 and Nab2) transcriptional coregulators (Le et al., 2005b; Desmazières et al., 2008; Baloh et al., 2009), which directly bind and repress Egr2 transcriptional activity (Russo et al., 1995; Svaren et al., 1996).
Establishment and maintenance of gene expression patterns depend upon epigenetic regulation, and studies of histone deacetylase (HDAC) function in oligodendrocytes and Schwann cells have highlighted their importance during myelination (Marin-Husstege et al., 2002; Ye et al., 2009; Liu and Casaccia, 2010; Chen et al., 2011; Jacob et al., 2011). Histone deacetylase activity is necessary for oligodendrocyte lineage progression, and Hdac1/Hdac2 control the transcriptional program of myelination and the survival of Schwann cells. Although histone deacetylases are often recruited to genes as components of larger chromatin remodeling complexes such as the Sin3a, CoREST, or NuRD complexes, the role of a specific chromatin-remodeling complex in peripheral nerve myelination has not been characterized.
Investigations into the molecular mechanism of Nab repression revealed two independent repression domains, one of which interacts with chromodomain helicase DNA-binding protein 4 (Chd4) (Mi2β) (Srinivasan et al., 2006; Mager et al., 2008). Chd4 catalyzes ATP-dependent nucleosome remodeling as part of the nucleosome remodeling and deacetylase (NuRD) complex (Denslow and Wade, 2007; Marfella and Imbalzano, 2007). The enzymatic activities of NuRD subunits combine chromatin remodeling, and histone deacetylation through the Hdac1/2 subunits of the NuRD complex. Although the NuRD complex was originally characterized as a repressive chromatin remodeling complex, it has also been found to promote gene expression (Williams et al., 2004; Yoshida et al., 2008; Miccio and Blobel, 2010). In this study, we have tested whether NuRD activity is required for peripheral nerve myelination by analyzing the morphological and gene expression defects caused by Schwann cell-specific ablation of Chd4.
Materials and Methods
Generation of mutant mice and genotyping.
Mi-2βLoxP/LoxP mice (designated as Chd4loxP/loxP) (Williams et al., 2004) and mP0TOTA(Cre) mice (designated as P0-cre transgenic mice) (Feltri et al., 1999) were bred and maintained under specific pathogen-free conditions with ad libitum food and water. To generate Chd4 mutant mice (Chd4loxP/loxP; P0-cre), female Chd4loxP/+; P0-cre mice were mated with male Chd4loxP/loxP mice. Genomic DNA from the resulting offspring was analyzed by PCR for the Cre transgene and the Chd4loxP/loxP allele. Chd4loxP/loxP mice were used as controls in these experiments (control Chd4 mice). All experiments were performed in strict accordance with experimental protocols approved by the University of Wisconsin School of Veterinary Medicine (Madison, WI). The founder mouse containing the P0-cre transgene was maintained on a FVB/N background (Feltri et al., 1999) and founder Chd4loxP/loxP was maintained on a 129/C57BL/6 mixed background. Hence, mice used in experiments contained a F2 mixed genetic background. Genotyping primer sequences are as follows: Chd4, forward, sense, 5′-CTC CAG AAG AAG ACG GCA GAT CT-3′; Chd4, reverse, antisense, 5′-GTC CTT CCA AGA GAG AGC AAG-3′; KG4R, antisense, 5′-CTT CCA CTG TGA CGT CCA GAC GCA-3′; mP0, 5′-CCA CCA CCT CTC CAT TGC AC-3′; Cre-2r, 5′-CAC GAC CGG CAA ACG GAC AGA AG-3′.
Accelerating Rotarod test.
Mice were trained and tested using a 1 d train/test regimen on a MED Associates ENV-575M Rotarod with an acceleration speed from 4 to 40 rpm in 300 s. Mice were placed on the rod rotating at 4 rpm. The rotation rate was then increased at 0.12 rpm/s to determine the latency time until the mice fell off the rod. Each mouse received one training session and then three trials with 20 min between each trial. At least five mice from each genotype were analyzed at each time point. All data were analyzed using ANOVA.
Quantitative RT-PCR.
For the developmental time points (P8, P15, and P30), RNA was purified using RNeasy Lipid Tissue Mini Kit (QIAGEN) according to the manufacturer's directions from sciatic nerves of Chd4 mutant and control mice. One microgram of total RNA from each sample was used to prepare cDNA as described previously (Lee et al., 1996). Quantitative RT-PCR was performed using Power SYBR Green Assays on the TaqMan Step One Plus detection system (Applied Biosystems). Relative amounts of each gene between samples were determined using the comparative Ct method (Livak and Schmittgen, 2001) and normalized to the relative levels of 18S rRNA. Primers used for analysis are included in Table 1.
List of mouse primers used in qRT-PCR experiments
Immunohistochemistry and Western blotting.
For immunohistochemical analysis of sciatic nerve, freshly dissected nerves were embedded in Tissue-Tek OCT compound (Sakura Finetek) and cut into 5 μm cryostat sections. For fluorescence immunohistochemistry, the sections were fixed for 15 min in 4% paraformaldehyde and then blocked in 5% goat serum/1% BSA for 1 h at room temperature. Incubation with primary antibody was performed overnight at 4°C in blocking solution, and secondary antibody incubation was performed at room temperature for 1 h. For Western immunoblotting, sciatic nerves were homogenized in lysis buffer (0.25 m Tris-HCl, pH 6.8, 20% glycerol, 4% SDS, 10% β-mercaptoethanol). The protein lysates were fractionated on a SDS-PAGE gel and transferred to nitrocellulose. The primary antibodies used were rabbit anti-Scip (1:1000; kind gift from D. Meijer, Rotterdam, the Netherlands), rabbit anti-Egr2/Krox20 (1:1000; Covance), rabbit anti-CHD3/4 (1:500; Santa Cruz; sc-11378), and mouse IgM anti-β-actin (1:1000; Cell Signaling Technology). Secondary antibodies used were anti-rabbit Alexa 488 and anti-goat Alexa 568 (1:1000; Invitrogen).
BrdU cell quantification.
Mice were injected with BrdU (50 mg/kg body weight) intraperitoneally and killed 1 h later. Nerves and skin were collected and processed for BrdU immunostaining. After counterstaining with bisbenzimide (Hoechst 33342; Sigma-Aldrich), BrdU positive cells and total nuclei were quantified by counting 10 random fields at 60× magnification. Processed nerves from three mice of each genotype were included in the quantification (∼4000 nuclei for each genotype).
Electron microscopy.
Sciatic nerves were immersion fixed in a solution of 2.5% glutaraldehyde, 2.0% paraformaldehyde in 0.1 m sodium phosphate buffer, pH 7.4, overnight at 4°C. The tissue was then postfixed in 1% osmium tetroxide in the same buffer for 2 h at room temperature. Following OsO4 postfixation, the samples were dehydrated in a graded ethanol series, and then further dehydrated in propylene oxide and embedded in Epon or Durcupan epoxy resin. Ultrathin sections were contrasted with Reynolds lead citrate and 8% uranyl acetate in 50% EtOH and observed with a Philips CM120 electron microscope and captured with a MegaView III side-mounted digital camera. The g ratio was calculated by division of the axonal diameter by total fiber diameter. Measurements of g ratio were performed using the NIS-Elements D3.10 software (Nikon) for sciatic nerve electron micrographs from three mice of each genotype (∼300 axons with a diameter of at least 1 μm). Statistical significance was evaluated using the Student's t test.
Chromatin immunoprecipitation and chromatin immunoprecipitation-chip assays.
In vivo chromatin immunoprecipitation (ChIP) assays were performed as previously described (Jang et al., 2006), and all data are representative of at least two independent experiments. Antibodies used in this study are as follows; rabbit anti-Egr2/Krox20 (Covance; PRB-236P), rabbit anti-Nab2 (Santa Cruz; sc-22815), rabbit anti-CHD3/4 (Santa Cruz; sc-11378), rabbit anti-Chd4 (kind gift from Paul Wade, Research Triangle Park, NC), goat anti-Mta2 (Santa Cruz; sc-9447), or control antibodies normal rabbit IgG (Millipore; 12–370) and normal goat IgG (Santa Cruz; sc-2028). Quantitative PCR was performed on the ChIP samples in duplicate to calculate the percentage recovery of a given segment relative to the total input, using the comparative Ct method (Livak, 2001). Primers used for analysis are included in Table 2. To combine ChIP with microarray analysis, amplicons were first generated from ChIP products by whole-genome amplification (Sigma-Aldrich). Labeling of the samples with Cy5 (experimental, anti-Egr2, anti-Nab2, anti-Chd4, or anti-Mta2) or Cy3 (control, 10% input) followed by microarray hybridization was performed as described previously (Jang and Svaren, 2009; Jang et al., 2010) by NimbleGen, using a custom microarray designed with isothermic probes tiled over gene loci identified in the Egr2lo/lo mouse (Le et al., 2005a). Coordinates of tiled regions are derived from the Rn4 genome build. Gaps in the tiling represent repetitive DNA regions for which unique probes could not be designed. The enrichment ratio of Cy5 to Cy3 was plotted on a log2 scale, and displayed as a moving average using a window size of five probes. Peak finding was performed using the NimbleScan software, with a false discovery rate (FDR) of 0.05. All raw data sets for the custom tiled array are available from the NCBI Gene Expression Omnibus website: accession numbers GSE30890 and GSE23648.
List of rat primers used in ChIP assays
Results
Deficiencies in motor coordination and abnormal hindlimb reflex in Schwann cell-specific Chd4 knock-out mice
To determine the role of the NuRD complex in PNS development, we created a conditional knock-out for Chd4 in Schwann cells by crossing Chd4loxP/loxP mice to a line expressing cre recombinase driven by regulatory elements from the Mpz/P0 gene (Feltri et al., 1999, 2002). The excised Chd4loxP allele lacks several exons containing the ATPase domain of Chd4 (Williams et al., 2004) and produces an unstable mRNA transcript leading to removal of Chd4 in cre-expressing cells. Immunohistochemical detection of Chd4 (Fig. 1A) in sciatic nerve shows the majority of the protein is located in the nucleus of wild-type nerve and is absent in mutant nerve.
Deficiencies in motor coordination and hindlimb reflex in Chd4 mutant mice. A, Immunohistochemistry of Chd4 (red) in the sciatic nerves of control and conditional knock-out mice at P8. Cell nuclei were counterstained with Hoechst (blue). Scale bar, 30 μm. B, Normal hindlimb postural reflex in a wild-type mouse (Chd4 f/f) characterized by spreading of the limbs. Abnormal reflex in Chd4 mutant mouse where hindlimbs are crossed and withdrawn (P55). C, Gross examination of sciatic nerve dissected from Chd4 KO and control mice at P30. D, RotaRod performance measuring latency to fall for 4-week-old mice. Three trials per session were measured with 20 min interval between trials. Statistical significance was determined using Student's t test: *p < 0.01; **p < 0.001. Error bars indicate SD.
Heterozygous Chd4 knock-out mice (Chd4loxP/+; P0-cre) are viable and fertile and have no apparent neuropathy phenotype. Chd4 mutant mice (Chd4loxP/loxP; P0-cre) and Chd4loxP/loxP mice lacking cre expression (hereafter designated Chd4 wild-type mice) are overtly indistinguishable from birth through the first 3 weeks of life. At that time, knock-out mice develop an abnormal hindlimb reflex. While wild-type mice spread their hindlimbs when suspended by the tail, Chd4 mutant mice react by crossing their hindlimbs and drawing them to the body (Fig. 1B). A similar abnormal hindlimb reflex has been observed in several other models of deficient myelination in the peripheral nervous system (Giese et al., 1992; Gillespie et al., 2000; He et al., 2010). Gross examination of sciatic nerve at 4 weeks revealed the typical white opaque appearance of control nerves and much thinner and translucent appearance of Chd4 mutant nerves, a phenotype indicative of hypomyelination (Fig. 1C).
To determine whether mutant mice differ in motor coordination, accelerating Rotarod performance was measured at 4 weeks of age. Rotarod performance was determined by placing mice on a rod rotating at 4 rpm. The rotation rate was then increased at 0.12 rpm/s to calculate the latency time until the mice fell off the rod. Rotarod training consisted of one training session and then three trials. A comparison of mean latency time between Chd4 mutant (30 ± 9 s) and wild-type mice (146 ± 8 s) shows that Chd4 ablation significantly affects motor coordination (Fig. 1D) before noticeable locomotion difficulties (p < 0.005). At 4 months, mice exhibit hindlimb muscle atrophy, wide-based gait, and difficulties in hindlimb coordination, and at 7 months the defects lead to dragging of posterior limbs. Together, these observations show absence of Chd4 function leads to progressive impairment of motor function.
Schwann cell developmental delay and radial sorting defects in Chd4 mutant sciatic nerve
To further characterize the myelination process in Chd4 knock-out mice, we performed electron microscopy analysis of sciatic nerve at postnatal day 8, 15, and 30. Peripheral myelination begins shortly after birth and is completed between the second and fourth week of life in mice (Friede and Samorajski, 1968; Ayers and Anderson, 1975) (for review, see Garbay et al., 2000). Compared with wild type, the nerve fibers of Chd4 mutant animals at P8 have a striking lack of myelinated axons (Fig. 2A,B). Moreover, the sparsely distributed axons with a myelin profile are hypomyelinated. By this stage in development, radial sorting of large-caliber axons (>1 μm) is complete in wild-type nerve. In contrast, examination of Remak bundles in mutant nerve reveals a significant number of large caliber axons that have not yet established a 1:1 relationship with individual Schwann cells (Fig. 2C, inset).
Chd4 mutant mice show a developmental delay. A, B, Electron microscopy analysis of sciatic nerve from wild-type and Chd4 mutant mice at P8, showing hypomyelination (arrows) and a radial sorting defect where large-caliber axons are still associated with Remak bundle axons (C, inset) (asterisks). Scale bars: 10 and 1 μm. D, The g ratio measures the ratio of axon diameter to its myelinated diameter. Unmyelinated Schwann cells are represented with a g ratio of 1 (box). Histogram error is given as mean SD for each bin. E, Axon classification into categories at P8: clustered-unmyelinated (dark gray), sorted-unmyelinated (light gray), or sorted-myelinated (black). P15 WT sciatic nerve (F) contrasts with hypomyelination (G, H) and radial sorting defects (I) in Chd4 mutant nerve. Scale bar, 2 μm.
To quantify myelin thickness, the g ratio was analyzed in P8 animals (Fig. 2D). The g ratio is measured by dividing the axon diameter by its myelinated diameter. The largest g ratio bin for a myelinated axon in wild-type animals peaked at 0.60 while mutant animals peaked at 0.70, a shift indicating hypomyelination of myelinated axons. In addition, axons completely lacking a myelin sheath (g ratio of 1) form the largest bin (59.0%) of axons in the mutant nerve. In contrast, the wild-type nerve has a small number of amyelinated axons (7.2%). The mean g ratio for wild-type axons is 0.70 compared with 0.88 in mutant mice and establishes a significant difference in myelin sheath thickness (p < 0.0001) between genotypes. This analysis confirms Chd4 knock-out mice have both thinner myelin profiles and fewer myelinated fibers.
The radial sorting defect was further characterized by classifying axons into three categories according to Schwann cell association and myelin state: clustered-unmyelinated, sorted-unmyelinated, or sorted-myelinated (Fig. 2E). Small-caliber axons are clustered into Remak bundles by nonmyelinating Schwann cells and are represented by the clustered-unmyelinated group. A radial sorting defect would also result in large-diameter axons appearing in this group. Sorted axons are associated with a single Schwann cell and have diameters >1 μm. At P8, the majority of wild-type nerve is composed of clustered-unmyelinated (52.0 ± 4.0%) and sorted-myelinated axons (44.9 ± 4.3%). In contrast, Chd4 knock-out nerve contains a significantly larger percentage of clustered-unmyelinated axons, 68.9 ± 3.2%, reflecting a radial sorting defect of large caliber axons, and relatively few myelinated axons (12.9 ± 0.9%). Sorted-unmyelinated axons also make up a significant percentage of axon classifications in the mutant nerve (18.19 ± 3.4%).
At P15, a peak stage of myelination, Chd4-deficient nerve exhibited similar developmental delays found at P8; however, the severity of the phenotype showed greater variability in radial sorting penetrance. Figure 2, G and H, highlights the persistence of amyelinated and hypomyelinated axons. Radial sorting defects are also seen at this developmental stage (Fig. 2I) and g ratio analysis (data not shown) continues to show myelin is significantly thinner than control nerve. Average axon diameters are decreased somewhat (wild type, 2.2 ± 0.7 μm, vs Chd4 mutant, 1.8 ± 0.6 μm; p < 0.0001). Together, these results indicate Chd4 is required for the process of axonal sorting in developing Schwann cells. In addition, the lack of a myelin sheath or thin myelin membranes in mutant Schwann cells that have established a 1:1 relationship with an axon may indicate Chd4 requirement for the timely initiation of myelination.
Deletion of Chd4 results in progressive defects in peripheral nerve myelin formation
Our characterization of the myelination time course in Chd4 knock-out mice was extended to P30, a late myelination/maintenance stage (Fig. 3). As noted above, Chd4 knock-out mice exhibit clear motor deficits beginning at 3 weeks of age. Our analysis at this time point indicates Chd4 knock-out mice have completed radial sorting as large-caliber axons are no longer associated with Remak bundles (Fig. 3B). The myelinated axons appear to have normal periodicity, if fewer wraps (Fig. 3C, inset). At P30, there is no statistical difference in overall axon diameter (wild type, 2.4 ± 0.9 μm, vs Chd4 mutant, 2.3 ± 0.8 μm; p < 0.3747). The g ratio plots show the myelin thickness in Chd4 mutant mice is consistently thinner across all axon diameters, while the slope of the trend line indicates the axon diameter is unchanged between control and mutant axons (Fig. 3D). A comparative analysis at these three time points is consistent with a developmentally delayed myelination process in Chd4-deficient nerve.
Ablation of Chd4 impairs the ability of Schwann cells to myelinate. Electron micrographs of sciatic nerve from control (A) and Chd4 cko (B) mice at P30. Magnification of myelin wraps show normal periodicity (C, inset). Scale bar, 0.1 μm. Scatter plot analysis of g ratio and axon diameter (D) shows the axon diameter is similar between genotypes; however, thinner myelin wraps axons of all diameters in Chd4 KO mice. Quantitation of the rate of dysmyelination indicates 24% of large-caliber axons contain abnormalities in myelination (E). Examples include amyelination, frayed myelin (F), or disorganized Schwann cell structures containing myelin debris (G). Scale bar: 1 and 2 μm, respectively. In addition, macrophage infiltration is characteristic of P30 nerve (H). Scale bar, 2 μm.
Examination of the myelinated fibers within P30 mutant nerve detected a number of abnormalities in the peripheral nervous system of Chd4-deficient mice. Classification analysis of large-caliber axons as myelinated or abnormal indicates 24.0% of mutant cells present an unusual phenotype (Fig. 3E). Abnormalities include lack of a myelin sheath, the appearance of large, distended Schwann cells filled with myelin debris, and the presence of vacuole-filled cells with split or frayed myelin layers (Fig. 3F,G). In addition, immune system activation becomes apparent at this stage with the infiltration of macrophages (Fig. 3H). No signs of onion bulbs or nonmyelinating Schwann cell defects are detectable in Chd4 mutant nerve at P30.
In summary, the myelination process in Chd4 knock-out animals is developmentally delayed and characterized by the continued presence of promyelinating cells and hypomyelination. Loss of Chd4 impairs defasciculation of large-caliber axons from Remak bundles through P15, indicating a postnatal role of Chd4 in the timing of these processes. While this phenotype is ameliorated by P30, the onset of inflammation and myelin degradation at P30 indicates that the ablation of Chd4 is incompatible with the maintenance of a myelin sheath in mutant Schwann cells.
Chd4 ablation leads to increased Schwann cell proliferation and deregulation of genes induced and repressed during development
Since many Schwann cells in Chd4 mutant mice fail to produce myelin, we hypothesized other attributes of delayed myelination would also be present. Mature myelinating Schwann cells typically undergo cell cycle arrest, but several gene deletions affecting myelination (including Egr2 and Nab mutants) also result in persistent proliferation (Zorick et al., 1999; Le et al., 2005a,b; Bremer et al., 2010; He et al., 2010). The proliferation status of Schwann cells was determined in P8 sciatic nerve. Mice were intraperitoneally injected with BrdU, and then killed 1 h later for analysis (Fig. 4). The proliferation rate was calculated from the ratio of BrdU-positive nuclei to total nuclei counterstained with bisbenzimide. While 3.9% of cells in wild-type nerve were BrdU positive, Chd4 knock-out mice had a higher level of proliferation, 5.86% (p < 0.019). In summary, the persistence of cell division indicates that Chd4 function is necessary for complete arrest of Schwann cell proliferation.
Persistence of Schwann cell proliferation. A–D, BrdU immunohistochemical analysis of P8 Chd4 cko sciatic nerve demonstrates an increased number of proliferating cells compared with control littermates. The BrdU-positive nuclei were counted, and proliferation was determined as a percentage of BrdU-positive nuclei compared with all Hoechst-stained nuclei in the nerve section. Scale bar, 30 μm. p < 0.019; n = 3 for each genotype.
Several independent studies have shown Egr2 in conjunction with Nab expression is critical for peripheral myelination (Le et al., 2005b; Desmazières et al., 2008; Baloh et al., 2009). Thus, knock-out mice with hypomyelination phenotype (e.g., Calcineurin B1, Sox10, YY1, Dicer, Hdac1/2) are commonly associated with a deficient level of Egr2 (Kao et al., 2009; Finzsch et al., 2010; He et al., 2010; Pereira et al., 2010; Yun et al., 2010; Chen et al., 2011; Jacob et al., 2011). However, in Chd4-deficient mice, Egr2/Krox20, and Nab2 maintain wild-type or greater mRNA expression levels throughout development (Fig. 5A). This is also true of protein expression for Egr2 (Fig. 5B). Importantly, the Chd4 mutant phenotype does not stem from reduced Egr2/Nab expression, suggesting that Chd4 is required for a step that is downstream of Egr2 induction itself.
Chd4 deletion results in deregulation of Schwann cell genes induced and repressed during development. A, B, The mRNA expression levels for the indicated genes were determined by quantitative RT-PCR from sciatic nerve samples at the P8, P15, and P30 time points. Expression data are presented as a ratio of Chd4 mutant compared with wild-type mRNA levels (C). P30 protein levels of Egr2 (D), Oct6 (E), activated β-catenin (G), and P4 levels of Pmp22 (F) mirror the levels found in the mRNA expression data. Error bars indicate SD. Line represents relative level in wild-type mice. *p < 0.05; **p < 0.01; ***p < 0.005.
Many of the genes typically expressed in immature or promyelinating Schwann cells remain elevated in peripheral nerve of Egr2- and Nab-deficient mice (Le et al., 2005a,b). Since Chd4 was found to mediate repression by Nab proteins (Srinivasan et al., 2006), we investigated whether such genes are similarly de-repressed in Chd4 mutant mice. During development, the timing of myelination is sensitive to transient Oct6/Pou3f1 expression at the promyelinating stage and subsequent repression following the initiation of myelination (Scherer et al., 1994; Bermingham et al., 1996; Blanchard et al., 1996; Jaegle et al., 1996). In Chd4 mutant nerve, expression of Oct6/Pou3f1 is significantly upregulated at both P8 (1.9-fold) and P30 (5.2-fold) (Fig. 5A,C). Several studies have identified other Schwann cell markers that are expressed during the immature stage of Schwann cell development and then are downregulated as development progresses. These genes include Sox2 (Le et al., 2005a,b), L1cam, Id2, the p75/nerve growth factor receptor (Jessen et al., 1990), the Egr family member, Egr1 (Topilko et al., 1997), Notch1 (Woodhoo et al., 2009), and c-Jun (Parkinson et al., 2004) (for review, see Jessen and Mirsky, 2008). Interestingly, these genes exhibit wild-type expression levels during the initiation of myelination at P8 (Fig. 5D). However, expression of these markers is significantly upregulated in Chd4 mutant mice at the P30 time point. De-repression of genes characteristic of immature Schwann cells late in developing nerve indicates Chd4 deficiency causes failure to maintain gene repression patterns typical of mature peripheral nerve.
Egr2 is required for developmental induction of lipid biosynthesis and major myelin genes (Topilko et al., 1994; Le et al., 2005a). We examined the expression levels of Mpz, the cholesterol biosynthesis genes, HMG CoA-reductase (Hmgcr), and squalene epoxidase (Sqle), Connexin 32 (Cx32), and Pmp22 in mutant animals by quantitative RT-PCR, and found that they were strongly downregulated relative to wild type (Fig. 5E) at multiple developmental time points. Furthermore, protein levels of Pmp22 are greatly reduced in the Chd4 knock-out at P4 (Fig. 5F). Myelin genes Pmp22 and Mpz are cooperatively activated by Egr2 and Sox10 (LeBlanc et al., 2007; Jang et al., 2010; Jones et al., 2011). However, quantitative RT-PCR analysis of Sox10 [and Oct6 and Egr2, which are regulated by Sox10 (Finzsch et al., 2010)] indicate that Sox10 levels are not reduced in Chd4 mutant mice.
NuRD components, Hdac1 and Hdac2, have previously been shown to regulate oligodendrocyte differentiation by disrupting the β-catenin-TCF interaction (Ye et al., 2009). Hdac-mediated control of activated β-catenin (ABC) is also critical in peripheral myelination (Jacob et al., 2011). However, analysis of Chd4 mutant nerve for elevated protein levels of ABC indicates this pathway is unchanged between genotypes (Fig. 5G).
The NuRD complex assembles on dynamically regulated genes in myelinating Schwann cells
Initial studies of the NuRD complex suggested that it was predominantly repressive, and studies of Chd4 binding supported its role in developmentally regulated gene repression through recruitment to chromatin by Egr2/Nab protein complexes (Srinivasan et al., 2006; Mager et al., 2008). However, studies of Chd4 function in other systems have shown that it can also be involved in gene activation (Williams et al., 2004; Yoshida et al., 2008; Miccio et al., 2010). Given that Egr2 and Nab knock-outs exhibit both reduced expression of myelin genes and de-repressed levels of immature Schwann cell genes (Le et al., 2005a,b), it was possible that Chd4 had a similar bimodal effect on transcription in peripheral nerve. Therefore, we examined the role of Chd4 in gene activation or repression by testing for Chd4 occupancy of developmentally regulated genes using in vivo ChIP. Immunoprecipitations were performed using pooled P15 rat sciatic nerve samples and performed on three independent chromatin preparations with antibodies targeting Egr2, Chd4, and another NuRD component, Mta2 (Fig. 6).
ChIP analysis for occupancy of Egr2, Chd4, and MTA2 on Egr2 activated and repressed genes. The bar graph shows ChIP analysis of NuRD subunits, Chd4 and Mta2 (A), and Egr2 (B) binding to activated and repressed genes in P15 rat sciatic nerve. Quantitative PCR was used to calculate percentage recovery for both experimental and control (IgG) immunoprecipitations relative to total input, and the mean of three independent ChIP experiments is shown. The negative control site is an Egr2 negative binding site in Ndrg1. Error is shown as ±SEM. *p < 0.05.
Gene expression is greatly reduced for myelin protein and lipid biosynthesis genes in Egr2, Nab protein, and Chd4-deficient nerve. Therefore, we further examined NuRD binding to the promoter regions of Mpz, Cx32, and Sqle, genes previously described to be positively regulated by Egr2 (Bondurand et al., 2001; Jang and Svaren, 2009; Jang et al., 2010) (Fig. 6A). The most robust binding of NuRD components was observed at the Sqle promoter, where Chd4 and Mta2 binding was observed at 6.6- and 6.5-fold over IgG control, respectively. Occupancy of Egr2 was also confirmed at this site to bind 38.7-fold higher than background. Significant accumulation of Egr2 was also detected in c-Jun, a gene repressed during myelination (Fig. 6B). Previous studies have shown Egr2 suppresses the JNK/c-Jun signaling pathway to regulate cell proliferation and death (Parkinson et al., 2004, 2008). ChIP analysis in myelinating sciatic nerve place binding of Egr2 and Mta2 of the NuRD complex 1 kb upstream of the c-Jun promoter. As a negative control, there was minimal binding of all three factors to a negative site in Ndrg1. These data demonstrate binding of both Egr2 and NuRD components to activated and repressed genes during peripheral nerve myelination.
To examine locus-wide binding of the NuRD complex in coordination with Egr2/Nab proteins, we examined protein binding by ChIP-chip array in myelinating sciatic nerve. The accumulation of the NuRD complex at multiple gene loci was assessed using in vivo ChIP samples hybridized to a custom tiled microarray described previously (Jang et al., 2010). ChIP assays for NuRD binding were performed in P15 rat sciatic nerve using two antibodies for Chd4 and an antibody for another NuRD component, Mta2. Previous ChIP-chip data for Egr2 (Jang et al., 2010) and an additional array for Nab2 were also used in this analysis. This array contains tiled probes for 50–100 kb regions around genes dynamically regulated during peripheral nerve myelination. Using NimbleScan peak finding software (FDR < 0.05), NuRD binding was found on 46.7% of Egr2-induced genes, such as myelin and lipid synthesis genes (Fig. 7A). However, binding to Egr2-repressed genes occurred at a rate of 60% to genes typically expressed in promyelinating Schwann cells such as Oct6, Sox2, or Id2 (Table 3) or on the negative regulator of myelination, c-Jun (Fig. 7B). Overall, ChIP-chip analysis of NuRD binding reveals that Chd4 binds to genes that are both activated and repressed by Egr2 activity.
NuRD complex components and Egr2 assemble on both activated and repressed genes during myelination. Chd4 binding is found on Sqle (A), and a negative regulator of myelination, c-Jun (B). ChIP-chip data for Egr2 (Jang et al., 2010) and two independent Chd4 antibodies were used to determine the binding location of Chd4 in P15 rat sciatic nerve. Data were analyzed using NimbleScan peak finding software. Peaks with a value of FDR <0.05 are colored in red. ChIP-chip analysis for occupancy of Egr2, Chd4, and MTA2 on the Egr2-repressed gene, c-Jun (B), confirms binding of these proteins to the same site upstream of the c-Jun promoter (box).
ChIP-chip analysis of NuRD binding
Discussion
Schwann cells of the peripheral nervous system require coordinated gene expression changes for myelin development to occur in a timely fashion. While epigenetic mechanisms play a significant role in a variety of developmental processes, distinct chromatin remodeling pathways that modulate gene expression changes during peripheral myelination have only recently begun to be investigated. Previous studies uncovered a novel mechanism for NAB repression of Egr2 target genes through interaction with the Chd4 subunit of the NuRD complex (Srinivasan et al., 2006; Mager et al., 2008). Our results now demonstrate that Chd4 ablation in the peripheral nervous system results in impaired Schwann cell differentiation leading to a developmental delay in myelination, reflected in the prevalence of promyelinating Schwann cells, hypomyelination, and enhanced proliferation at P8. Inefficient initiation of the myelin-producing stage in Schwann cell development indicates loss of Chd4 results in delayed and incomplete Schwann cell differentiation. The myelination status deteriorates considerably by P30, resulting in significant ataxia, myelin degeneration, and altered gene expression patterns, indicating that Chd4 may also play a role during later stages of Schwann cell myelination for the maturation and homeostasis of the myelin sheath beyond P15.
Although the association of Chd4 with NAB coregulators provided the mechanistic basis for this study, the resulting phenotype is significantly less severe than that caused by deletion of both NAB genes, or by knock-in of a NAB-resistant allele of Egr2 (Le et al., 2005b; Desmazières et al., 2008; Baloh et al., 2009). One possible explanation could be redundancy with Chd3. Chd4 is highly similar to Chd3, and both proteins have been shown to be part of the NuRD complex (Ramírez and Hagman, 2009; Y. Wang et al., 2009) and have been shown to interact with Nab2 (Srinivasan et al., 2006). However, using an antibody that detects both Chd3 and Chd4, there seems to be little background expression of Chd3 after deletion of Chd4, although it is possible that residual levels could compensate to some degree. In addition, NAB proteins have two independent repression domains, one of which exerts its activity in an Hdac- and Chd4-independent manner (Srinivasan et al., 2006). Therefore, it is anticipated that NAB proteins retain some level of repressive activity in the absence of NuRD activity.
Our studies of Chd4-deficient nerve revealed not only de-repression of genes that are normally repressed in mature myelinating Schwann cells, but also reduced levels of several critical myelin genes in sciatic nerves from knock-out mice. Accordingly, ChIP analysis in myelinating sciatic nerve detected binding of Chd4 and another NuRD subunit (Mta2) at promoters of repressed genes, consistent with direct repression of negative regulators by the NuRD complex during development. This analysis also detected binding of Chd4 and MTA2 to promoters that are induced during myelination. Our findings are similar in many respects to analysis of Nab1/Nab2 double knock-out mice (Le et al., 2005b), which revealed that Nab proteins are required for both gene activation and gene repression by Egr2.
Other studies of Chd4 function have shown direct requirement of the NuRD complex to both activate and repress transcription in hematopoietic development (Yoshida et al., 2008; Miccio and Blobel, 2010; Miccio et al., 2010). Although we detect binding of NuRD components at both activated and repressed genes, it is possible that H3K4 trimethylation at active promoters prevents repressive remodeling by the NuRD complex (Nishioka et al., 2002; Zegerman et al., 2002). In addition, the activation state of a given promoter may reflect a balance between deacetylation by the NuRD complex, and acetylation by other coactivators (CBP/p300), which are recruited by Egr2 and associated factors. Interestingly, genomic profiling of Hdac binding has revealed widespread binding of Hdac1/2 to active genes (Z. Wang et al., 2009). Therefore, it is possible that histone deacetylation at regulatory regions may precede positive or negative methylation (K4 vs K9/K27) at different genes as myelination proceeds.
Recent studies have shown that peripheral nerve myelination depends upon activity of Hdac1/2 (Chen et al., 2011; Jacob et al., 2011). The role of these histone deacetylases is mechanistically complex since they are constituents of multiple complexes (NuRD, Sin3a, CoRest). In addition, it is clear that they also have important nonhistone substrates. For example, in glial development, Hdac1 and Hdac2 regulate oligodendrocyte differentiation by disrupting the β-catenin-TCF interaction (Ye et al., 2009), and HDAC-mediated deacetylation of NF-κB is a determinant of gene regulation (Chen et al., 2011). The phenotype of the Chd4-deficient peripheral nerve is significantly milder compared with Hdac1/2-deficient mice, which exhibit failure to myelinate and lack of Egr2 expression (Chen et al., 2011; Jacob et al., 2011). We did not observe any obvious level of apoptosis exhibited by Hdac-deficient mice (Jacob et al., 2011), and the level of Oct6 expression indicates that NF-κB acetylation status is likely not perturbed in Chd4-deficient mice. In addition, Western blotting indicated no significant difference in β-catenin at P30. This likely reflects the fact that NuRD complexes mediate only a subset of Hdac-regulated events.
Substantial progress has been made in identifying transcription factors that coordinate gene expression during myelination. However, for many such studies, a common feature of the knock-out phenotype is reduced Krox20/Egr2 expression, as observed in the Sox10, Calcineurin B1, Hdac, Oct6, dicer, and YY1 knock-out studies of peripheral nerve (Blanchard et al., 1996; Kao et al., 2009; Finzsch et al., 2010; He et al., 2010; Pereira et al., 2010; Yun et al., 2010; Chen et al., 2011; Jacob et al., 2011). Importantly, the Chd4 knock-out phenotype does not stem from reduced Egr2 expression, suggesting that gene regulation by Chd4 is required downstream of (or parallel to) Egr2 activity.
While development favors induction of a myelinated nerve state, these conditions must be quickly reversible in response to nerve injury. During nerve injury, Schwann cells undergo a process of dedifferentiation, and suppress myelin genes while inducing negative regulators (Jessen and Mirsky, 2008). For the nervous system to complete a full recovery and remyelinate, the balance of transcriptional control must then swing back to promoting Schwann cell myelination. Ineffective suppression of negative regulators significantly affects both timing and extent of remyelination. The molecular pathways controlling repression of dedifferentiation factors are not well understood. However, recent studies have elucidated the importance of epigenetic regulation of myelin gene expression. Shen et al. (2008) showed remyelination efficiency in oligodendrocytes decreases with age due to the reduced ability of senescent cells to recruit HDAC proteins to the promoters of differentiation inhibitors and neural stem cell markers. Of note, the NuRD complex is one of the major HDAC-containing chromatin-remodeling complexes. Moreover, subunits of the NuRD complex are prone to silencing during aging leading to changes in chromatin modification, structure, and protein recruitment (Pegoraro and Misteli, 2009). Improving our understanding of the epigenetic mechanisms that control myelin formation and maintenance, including recruitment of chromatin remodeling complexes, will be critical to elucidate the genomic programming required for myelination.
Footnotes
This work was supported by National Institutes of Health Grant HD41590 (J.S.) and National Institute of Child Health and Human Development Grant P30 HD03352 (core grant to the Waisman Center). We thank Albee Messing and Katja Georgopoulos for generously providing the P0-CRE and the Chd4 floxed mice, respectively; Camila Lopez-Anido for mouse work assistance; Paul Wade for a Chd4 antibody; Dies Meijer for Oct6 antibodies; and Karla Knobel, the University of Wisconsin Electron Microscope Facility, and David Gamm for providing microscopy resources.
The authors declare no competing financial interests.
- Correspondence should be addressed to Dr. John Svaren, Waisman Center, University of Wisconsin–Madison, 1500 Highland Avenue, Madison, WI 53705. jpsvaren{at}wisc.edu
