Article Information
- Received August 31, 2011
- Revision received December 17, 2011
- Accepted January 9, 2012
- First published February 29, 2012.
- Version of record published February 29, 2012.
Author Information
- Hatim A. Zariwala1,2,
- Bart G. Borghuis1,
- Tycho M. Hoogland3,
- Linda Madisen2,
- Lin Tian1,
- Chris I. De Zeeuw3,4,
- Hongkui Zeng2,
- Loren L. Looger1,
- Karel Svoboda1, and
- Tsai-Wen Chen1
- 1Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147,
- 2Allen Institute for Brain Science, Seattle, Washington 98103,
- 3Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), 1105 BA Amsterdam, The Netherlands, and
- 4Department of Neuroscience, Erasmus Medical Center, 3015 GE Rotterdam, The Netherlands
Author contributions
Author contributions: H.Z., K.S., and T.-W.C. designed research; H.A.Z., B.G.B., T.M.H., and T.-W.C. performed research; L.M., L.T., C.I.D.Z., H.Z., L.L.L., and T.-W.C. contributed unpublished reagents/analytic tools; B.G.B., T.M.H., and T.-W.C. analyzed data; H.A.Z., B.G.B., T.M.H., H.Z., L.L.L., K.S., and T.-W.C. wrote the paper.
Disclosures
- Received August 31, 2011.
- Revision received December 17, 2011.
- Accepted January 9, 2012.
This work was supported by the Howard Hughes Medical Institute and the Allen Institute for Brain Science, and by an NIH grant (DA028298) to H.Z. We thank M. Stryker and C. Niell for advice in visual cortex imaging, L. Petreanu for suggestions with the experimental design, S. Wang and D. Kim for comments on this manuscript, K. Smith and A. Hu for surgical and histological support, D. Flickinger for help with microscope design, Q. Sun and E. Schreiter for help with measuring intracellular GCaMP3 concentration, and S. Sternson for providing Cre virus.
The authors declare no competing financial interests.
- Correspondence should be addressed to either of the following: Hongkui Zeng at the above address, hongkuiz{at}alleninstitute.org; or Tsai-Wen Chen at the above address, chent{at}janelia.hhmi.org
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