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Articles, Systems/Circuits

Mapping Brain Metabolic Connectivity in Awake Rats with μPET and Optogenetic Stimulation

Panayotis K. Thanos, Lisa Robison, Eric J. Nestler, Ronald Kim, Michael Michaelides, Mary-Kay Lobo and Nora D. Volkow
Journal of Neuroscience 10 April 2013, 33 (15) 6343-6349; https://doi.org/10.1523/JNEUROSCI.4997-12.2013
Panayotis K. Thanos
1Laboratory of Neuroimaging, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland 20852,
2Behavioral Neuropharmacology and Neuroimaging Laboratory, Medical Department, Brookhaven National Laboratory, Upton, New York 11973,
3Department of Psychology, Stony Brook University, Stony Brook New York 11794,
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Lisa Robison
1Laboratory of Neuroimaging, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland 20852,
3Department of Psychology, Stony Brook University, Stony Brook New York 11794,
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Eric J. Nestler
5Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029
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Ronald Kim
1Laboratory of Neuroimaging, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland 20852,
2Behavioral Neuropharmacology and Neuroimaging Laboratory, Medical Department, Brookhaven National Laboratory, Upton, New York 11973,
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Michael Michaelides
5Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029
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Mary-Kay Lobo
4Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, and
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Nora D. Volkow
1Laboratory of Neuroimaging, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland 20852,
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Abstract

Positron emission tomography (PET) with [18F]2-fluoro-2-deoxy-d-glucose was used to measure changes in regional brain glucose metabolism (BGluM) in response to optogenetic stimulation (using the excitatory channelrhodopsin-2) of the nucleus accumbens (NAc) in awake rats. We demonstrated not only increases in BGluM that correlated with c-Fos expression in the region of stimulation, but also BGluM increases in the ipsilateral striatum, periaqueductal gray, and somatosensory cortex, and in contralateral amygdala, ventral pallidum, globus pallidus, and hippocampus, as well as decreases in BGluM in regions of the default mode network (retrosplenial cortex and cingulate gyrus) and secondary motor cortex. Additional exploration of c-Fos expression in regions found to be activated by PET results found corroborating evidence, with increased c-Fos expression in the ipsilateral somatosensory cortex, contralateral amygdala and globus pallidus, and bilateral periaqueductal gray. These findings are consistent with optogenetic excitation of the area of stimulation (NAc), as well as with stimulatory and inhibitory effects on downstream regions. They also confirm the utility of PET imaging to monitor connectivity in the awake rodent brain.

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The Journal of Neuroscience: 33 (15)
Journal of Neuroscience
Vol. 33, Issue 15
10 Apr 2013
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Mapping Brain Metabolic Connectivity in Awake Rats with μPET and Optogenetic Stimulation
Panayotis K. Thanos, Lisa Robison, Eric J. Nestler, Ronald Kim, Michael Michaelides, Mary-Kay Lobo, Nora D. Volkow
Journal of Neuroscience 10 April 2013, 33 (15) 6343-6349; DOI: 10.1523/JNEUROSCI.4997-12.2013

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Mapping Brain Metabolic Connectivity in Awake Rats with μPET and Optogenetic Stimulation
Panayotis K. Thanos, Lisa Robison, Eric J. Nestler, Ronald Kim, Michael Michaelides, Mary-Kay Lobo, Nora D. Volkow
Journal of Neuroscience 10 April 2013, 33 (15) 6343-6349; DOI: 10.1523/JNEUROSCI.4997-12.2013
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