Article Information
- Received February 22, 2012
- Revision received October 28, 2012
- Accepted November 7, 2012
- First published January 9, 2013.
- Version of record published January 9, 2013.
Author Information
- Mackenzie W. Amoroso1,2,*,
- Gist F. Croft1,2,*,
- Damian J. Williams3,
- Sean O'Keeffe4,
- Monica A. Carrasco4,
- Anne R. Davis5,
- Laurent Roybon1,2,
- Derek H. Oakley1,2,
- Tom Maniatis4,
- Christopher E. Henderson1,2, and
- Hynek Wichterle1,2
- 1Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research, New York, New York 10032;
- 2Departments of Pathology, Neurology, and Neuroscience, Center for Motor Neuron Biology and Disease and Columbia Stem Cell Initiative, Columbia University, New York, New York 10032;
- 3Department of Physiology and Cellular Biophysics, Columbia University, New York, New York 10032;
- 4Department of Biochemistry and Biophysics, Columbia University, New York, New York 10032;
- 5Department of Obstetrics and Gynecology, Columbia University Medical Center, New York, New York 10032
Author contributions
Author contributions: M.W.A., C.E.H., and H.W. designed research; M.W.A., G.F.C., D.J.W., S.O., and H.W. performed research; G.F.C., M.A.C., A.R.D., L.R., D.H.O., and T.M. contributed unpublished reagents/analytic tools; M.W.A., D.J.W., and H.W. analyzed data; M.W.A., C.E.H., and H.W. wrote the paper.
↵*M.W.A. and G.F.C. contributed equally to this work.
Disclosures
- Received February 22, 2012.
- Revision received October 28, 2012.
- Accepted November 7, 2012.
This work was supported by Project A.L.S., P2ALS, National Institutes of Health (NIH) GO Grant 5 RC2 NS069395-02, NYSTEM Grant C024415, and the Dr. Leigh G. Cascarilla Postdoctoral Fellowship in Stem Cell Research (L.R). NIH DP1OD003930 (T.M.); the ALS Association (M.A.C.); and The ALS Therapy Alliance (S.O.). We thank Amy MacDermott for introducing calcium-imaging approaches into our work and Kevin Eggan for open sharing of reagents and ideas throughout this study. We are grateful to F.P. Di Giorgio for the HUES3 HB9 reporter line; Susan Brenner-Morton and Thomas M. Jessell for graciously providing ISL1, HB9, RALDH2, and FOXP1 antibodies and motor neuron expertise; Lee Rubin for the human Smo agonist; David J. Kalher and Mathew Darwin-Zimmer (NYSCF) for help with FACS; R.M. Myers and F. Pauli, for performing RNA sequencing; and OB/GYN staff and the anonymous donors for tissue samples. We benefited from many helpful discussions with members of the Project A.L.S.; Wichterle, Henderson, and Eggan laboratories; and unwavering support from Valerie and Meredith Estess (Project A.L.S.).
The authors declare no competing financial interests.
- Correspondence should be addressed to Hynek Wichterle, Columbia University, College of Physicians and Surgeons, PS14-401 630 W. 168th Street. New York, NY 10032. hw350{at}columbia.edu
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