Figure 4. Expression of GlyR α1, α3, or α4a subunits restored the normal touch response in Dhx37-deficient embryos. A, Injection of MO2, which blocks the splicing of Dhx37, into wild-type embryos led to a dorsal bend response. Coinjection of either GlyR α1, α3, or α4a subunit RNAs with MO2 decreased the abnormal behavior and restored the normal response. Mixed application of GlyR α1, α3, and α4a subunit RNAs with MO2 also restored the normal escape. Neither GlyR α2 nor α4b subunit RNAs had an effect on the motor recovery. The histogram represents the ratio of embryos exhibiting level 1, 2, or 3. B, All of the wild-type embryos injected with control MO exhibited a lateral turn in the escape response. Injection of GlyR α1, α3, or α4a MOs alone induced the level 2 response (α1 MO: 8%; α3 MO: 21%; α4a MO: 65%). Mixed application of α1 and α3 MOs, α1 and α4a MOs, or α3 and α4a MOs increased the level 2 response rate compared with single MO injections. Most of the embryos injected with all three MOs (α1, α3, and α4a) exhibited the severe level 3 phenotype. Injection of GlyR βa MO did not affect the touch response. GlyR βb MO-injected embryos displayed level 3 motor deficits.