Brief Communications

Endothelial VEGF Sculpts Cortical Cytoarchitecture

Suyan Li, Katharina Haigh, Jody J. Haigh and Anju Vasudevan
Journal of Neuroscience 11 September 2013, 33 (37) 14809-14815; https://doi.org/10.1523/JNEUROSCI.1368-13.2013

Abstract

Current models of brain development support the view that VEGF, a signaling protein secreted by neuronal cells, regulates angiogenesis and neuronal development. Here we demonstrate an autonomous and pivotal role for endothelial cell-derived VEGF that has far-reaching consequences for mouse brain development. Selective deletion of Vegf from endothelial cells resulted in impaired angiogenesis and marked perturbation of cortical cytoarchitecture. Abnormal cell clusters or heterotopias were detected in the marginal zone, and disorganization of cortical cells induced several malformations, including aberrant cortical lamination. Critical events during brain development-neuronal proliferation, differentiation, and migration were significantly affected. In addition, axonal tracts in the telencephalon were severely defective in the absence of endothelial VEGF. The unique roles of endothelial VEGF cannot be compensated by neuronal VEGF and underscores the high functional significance of endothelial VEGF for cerebral cortex development and from disease perspectives.

Introduction

Vascular endothelial growth factor (VEGF or VEGF-A), a powerful secreted signaling protein, is indispensable for physiological angiogenesis (Carmeliet et al., 1996; Ferrara et al., 1996), vascular homeostasis (Lee et al., 2007), and pathological angiogenesis (Hicklin and Ellis, 2005). Current notions of brain development support the view that neuronal progenitors and/or neurons are the principal source of VEGF (Ogunshola et al., 2000) that act by binding to VEGF receptors expressed on vascular endothelial cells (Mustonen and Alitalo, 1995) to stimulate angiogenesis. High VEGF expression by neuronal progenitors in the embryonic ventricular zone (VZ) induces angiogenesis by paracrine mechanisms (Breier et al., 1995; Ogunshola et al., 2002). Consequently, conditional deletion of Vegf from neuronal progenitors resulted in impaired angiogenesis and severe disruption of neuronal development at early embryonic stages (Haigh et al., 2003; Raab et al., 2004). Although VEGF expression in endothelial cells has been reported during brain development (Virgintino et al., 2003) and plays an important role in adult endothelial homeostasis (Lee et al., 2007), its role during embryonic brain development remains unknown. Our studies have challenged notions of cerebral vascularization, which imply that blood vessels sprout passively to meet metabolic demands of growing neuronal populations and offered new perspectives on intrinsic regulation of angiogenesis in the embryonic telencephalon (Vasudevan et al., 2008). Periventricular vessels are distinct from pial vessels based on anatomical location, growth patterns, and gene expression profiles (Vasudevan et al., 2008; Won et al., 2013). In addition, the periventricular vascular network provides support and critical guidance cues to instruct neuronal migration in the developing telencephalon (Won et al., 2013). Because the propagation of the periventricular angiogenesis gradient corresponds to the neurogenetic gradient with respect to direction (Vasudevan et al., 2008) and the gradient of VEGF expression mirrors the neurogenetic gradient (Virgintino et al., 2003), we selectively deleted Vegf from endothelial cells to investigate the functional significance of endothelial cell-derived VEGF in the embryonic telencephalon. Our results unveil a unique role for endothelial VEGF in regulating cortical histogenesis and highlight the complexity of neurovascular cross talk and interactions in time and space that is of great significance for neuronal development and disease.

Materials and Methods

Animals.

To selectively delete Vegf in endothelial cells, Tie2-cre transgenic mice (males) were crossed to Vegf floxed (Vegffl/fl) mice (females) to generate Tie2-cre; Vegf fl/+ mice (males). These were further crossed with Vegffl/fl mice (females) to obtain the Vegf conditional knock-out, hereafter referred to as Vegf ΔTie2-Cre mice. The Tie2-cre transgene is known for uniform expression of the cre-recombinase in endothelial cells during embryogenesis (Schlaeger et al., 1997; Koni et al., 2001). All animal procedures were approved by the animal care and use committee of Ghent University.

Bromodeoxyuridine (BrdU) labeling.

A single BrdU injection (50 μg/g) was administered to pregnant dams carrying E13 or E17 mice. Embryonic brains were removed after 2 h, immersed in zinc fixative (BD Biosciences PharMingen) for 24 h, and processed for paraffin wax histology. BrdU immunohistochemistry was performed on coronal, 20 μm sections. Double labeling was performed with a mouse monoclonal antibody to BrdU (1:75, 347580, BD Biosciences PharMingen) and biotinylated isolectin B4 (1:40, L2140, Sigma).

Histology and Immunohistochemistry.

E13, E15, and E17 brains were fixed in zinc fixative (BD Biosciences PharMingen) for 24 h and processed for paraffin histology. Histological stainings with hematoxylin (Vector Laboratories) and eosin (Sigma) were performed on 8 μm coronal sections, and lectin histochemistry (with biotinylated isolectin B4, 1:40, Sigma) as well as immunohistochemistry were performed on 20 μm sections. Primary antibodies used for immunohistochemistry were as follows: mouse monoclonal antibody to RC2 (1:5, MAB5740, Millipore), goat polyclonal antibody to CHL1 (1:50, AF2147, R&D Systems), rat monoclonal antibody to CD31/PECAM-1 (1:50, 550274, BD Biosciences PharMingen), and rabbit polyclonal antibodies to laminin (1:10, L9393, Sigma), Ki67 (1:30, HPA000451, Sigma), microtubule associated protein 2 (MAP2; 1:50, M3696, Sigma), Tbr1 (1:25, ab31940, Abcam), GAD65/67 (1:50, AB1511, Millipore), calbindin (1:30, AB1778, Millipore), calretinin (1:30, 7699/3H, Swant), followed by secondary detection with AlexaFluor conjugates (Invitrogen). DAPI (Vector Laboratories) was used to label nuclei. High-magnification images were obtained from an FSX100 microscope (Olympus).

Morphometry.

A stereological point grid was superimposed on digital images of biotinylated isolectin-B4+ vessels. The ratio between points falling on blood vessels and on brain tissue was calculated for each section, and average values were obtained for a given brain.

Statistics.

Statistical significance of differences between groups was analyzed by two-tailed Student's t test (Prism; GraphPad software). Results were expressed as mean ± SD, and statistical significance was reported at p < 0.05.

Results

Impaired angiogenesis in VegfΔTie2-Cre embryos

To test whether deletion of Vegf from endothelial cells affected telencephalic angiogenesis, we first investigated vascular profiles in Vegffl/fl (wild-type littermates) and Vegf ΔTie2-Cre embryos at early (E13) and late (E17) embryonic stages. By E13, the periventricular vessel gradient from ventral to dorsal telencephalon was clearly visible in Vegf ΔTie2-Cre embryos (Fig. 1B). Periventricular vessel numerical density (expressed as percentage) showed no significant differences in E13 Vegffl/fl (15.54) and VegfΔTie2-Cre (14.79) telencephalon (Fig. 1A,B, n = 8, mean ± SD, p > 0.05). However, by E17, vessel density was significantly reduced in VegfΔTie2-Cre telencephalon (21.65) compared with Vegffl/fl telencephalon (26.75) (Fig. 1C,D, n = 8, mean ± SD, p < 0.05). Reduction in vessel densities was more prominent in the dorsal telencephalon (pallium) of VegfΔTie2-Cre embryos (Fig. 1D). Labeling with multiple markers of vessel components, laminin (Fig. 1E,F) and CD31/PECAM-1 (Fig. 1G,H) confirmed the reduction in E17 VegfΔTie2-Cre pallium. Similar to vessel densities, endothelial cell proliferation was comparable in E13 Vegffl/fl and VegfΔTie2-Cre telencephalon (Fig. 1I,J,M). Endothelial cell proliferation continued independent of neuroepithelial cell proliferation in both neurogenic and postmitotic regions of Vegffl/fl telencephalon at E17 (Fig. 1K). Endothelial cell proliferation in postmitotic regions was affected such that the 2.0 h BrdU labeling index was significantly reduced in VegfΔTie2-Cre telencephalon at E17 (Fig. 1L,M). Dilated and abnormally stretched vessels were also observed in the VegfΔTie2-Cre telencephalon (Fig. 1N).

Figure 1.
Figure 1.

Impaired angiogenesis in VegfΔTie2-Cre embryos. A, B, Isolectin B4+ periventricular vessel gradient (white arrows) in E13 Vegffl/fl (A) and VegfΔTie2-Cre (B) telencephalon. C, D, Fewer isolectin B4+ vessels in E17 VegfΔTie2-Cre pallium (D, red arrows) compared with Vegf fl/fl pallium (C, white arrows). E, F, Fewer laminin+ vessels in E17 VegfΔTie2-Cre pallium (F, red arrows) compared with Vegffl/fl pallium (E, black arrows). G, H, Fewer PECAM-1+ endothelial cells in E17 VegfΔTie2-Cre pallium (H, red arrows) compared with Vegffl/fl pallium (G, white arrows). I, J, Double labeling of endothelial cells with isolectin B4 and BrdU (white arrows point to colabel in yellow) shows no differences in endothelial cell proliferation in E13 Vegffl/fl and VegfΔTie2-Cre telencephalon. K, Endothelial cells proliferate in neurogenic and postmitotic regions of E17 Vegffl/fl telencephalon (white arrows point to coexpressing cell nuclei that appear in yellow). L, Endothelial cells proliferate only in neurogenic regions (white arrows), and there is lack of BrdU-isolectin B4 colabel in postmitotic regions (red arrows) of VegfΔTie2-Cre telencephalon. M, Endothelial cell BrdU labeling index was comparable in E13 Vegffl/fl and VegfΔTie2-Cre telencephalon (n = 8, mean ± SD, p > 0.05) and significantly decreased in E17 VegfΔTie2-Cre telencephalon compared with Vegffl/fl telencephalon (n = 8, mean ± SD, *p < 0.05). N, Dilated and stretched vessels were detected in the E17 VegfΔTie2-Cre telencephalon. Scale bars: A (applies to B–D, I–L), 100 μm; E (applies to F), 75 μm; G (applies to H), 50 μm; N, 25 μm. n = 8.

Cortical malformations in VegfΔTie2-Cre embryos

The morphology of VegfΔTie2-Cre telencephalon at E13 appeared normal in histological stainings and comparable to Vegffl/fl telencephalon (data not shown). But, by E17, while ordered cortical laminar organization was observed in Vegffl/fl embryos (Fig. 2A), deletion of endothelial VEGF had far-reaching effects with marked perturbation of cortical cytoarchitecture in VegfΔTie2-Cre embryos (Fig. 2B–H). The malformations included the following: polymicrogyria overlying heterotopic cortical plate (CP) (Fig. 2B,C), marginal zone (MZ) heterotopias (Fig. 2D), cortical dysplasia, including split CP mislocated to intermediate zone (IZ) combined with MZ heterotopia and reduced subventricular zone (SVZ) (Fig. 2E), dispersion of CP cells into deeper layers of IZ (Fig. 2F), and abnormally enlarged IZ (Fig. 2G), all of which caused severe disruption of cortical lamination. These defects were present both at rostral (Fig. 2B–G) and caudal (Fig. 2H) levels of VegfΔTie2-Cre telencephalon. Our data offer novel evidence to the important role of endothelial cell-derived VEGF in periventricular angiogenesis with significant consequences for cortical development.

Figure 2.
Figure 2.

Cortical malformations in E17 VegfΔTie2-Cre embryos. A, Hematoxylin and eosin staining showed ordered cortical laminar organization in Vegffl/fl embryos. B–H, Hematoxylin and eosin staining revealed marked perturbation of cortical cytoarchitecture in VegfΔTie2-Cre embryos. A–H, Boxed areas have been magnified (60×) and cortical layers labeled. Scale bars: A (applies to B–H), 100 μm; insets (A–H), 50 μm. n = 8.

Perturbed neuronal proliferation, migration, and lamination in VegfΔTie2-Cre telencephalon

Because E15 is an age midway through mouse cortical neurogenesis and migration, we investigated whether vascular and neuronal-specific defects could be observed by this stage. Endothelial cells/vessels in the VegfΔTie2-Cre pallium were reduced as observed by labeling with PECAM-1 (Fig. 3A,B), laminin (Fig. 3C,D), and isolectin B4 (Fig. 3E,F) markers. To define the identity of the cells that accounts for the defective cortical cytoarchitecture, we examined the expression of markers specific for neuroepithelial precursors and differentiating neurons. Progenitor cells in neurogenic regions around the lateral ventricles were identified in Vegffl/fl telencephalon by Ki67 immunoreactivity (Fig. 3G). In contrast, abnormally proliferating progenitors were observed outside the neurogenic zone along the full length of the VegfΔTie2-Cre pallium (Fig. 3H). Spatial patterns of neuronal differentiation were examined by expression of MAP2 and cortical neuron population marker Tbr1, which marks CP, subplate (SP), and layer VI in wild-type embryos (Fig. 3I–M). MAP2-immunoreactive postmitotic neurons were detected within the SVZ in VegfΔTie2-Cre embryos, revealing evidence of perturbed neuronal migration (Fig. 3J,K). Tbr1 immunoreactivity revealed a continuous uniform band of Tbr1-positive cells in the CP of E15 Vegffl/fl embryos (Fig. 3L). On the other hand, abnormally thicker and thinner bands of Tbr1-positive cells were detected in the CP of VegfΔTie2-Cre embryos (Fig. 3M). The radial glial scaffold that extends fibers from ventricular to pial surface in Vegffl/fl embryos (Fig. 3N) and is essential for guiding migrating cortical neurons was shortened and disrupted in VegfΔTie2-Cre embryos (Fig. 3O). We also examined the tangential stream of GABA neurons that migrate from basal to dorsal telencephalon in Vegffl/fl and VegfΔTie2-Cre embryos (Fig. 3P,Q). GAD65/67 immunoreactivity depicted a reduced GABA neuron stream in the dorsal telencephalon of VegfΔTie2-Cre embryos (Fig. 3Q). To identify whether specific subsets of GABA neurons were affected, we used calbindin and calretinin immunohistochemistry (Fig. 3R–U). Calbindin-positive and calretinin-positive neurons were significantly reduced in VegfΔTie2-Cre pallium (Fig. 3S,U). Several layers of the dorsomedial telencephalon labeled heavily for calretinin in Vegffl/fl embryos (Fig. 3T); in contrast, calretinin-positive cells were significantly reduced dorsomedially in VegfΔTie2-Cre embryos (Fig. 3U). Calretinin-positive cells formed a single continuous row and labeled Cajal-Retzius cells of the MZ and SP in Vegffl/fl embryos (Fig. 3V). The uniform layer of the MZ in VegfΔTie2-Cre telencephalon was disturbed at places, calretinin-positive cells were seen outside the MZ, and calretinin-labeled fibers in the SP were thinner (Fig. 3W). Axonal tracts labeled by neural cell adhesion molecule, CHL1 (Fig. 3X,Y) occasionally narrowed and appeared displaced in the VegfΔTie2-Cre dorsal telencephalon at this stage (Fig. 3Y).

Figure 3.
Figure 3.

Perturbation of vascular and neuronal development in E15 VegfΔTie2-Cre telencephalon. A–F, Reduced PECAM-1+ endothelial cells (B, yellow asterisk), laminin+ vessels (D, red arrows), and isolectin B4+ vessels (F, yellow arrows) in E15 VegfΔTie2-Cre pallium (B, D, F) compared with Vegffl/fl pallium (A, C, E). C, E, Orange arrows indicate well-formed vascular network in Vegffl/fl pallium. G, H, Ki67 labeling marks the proliferating progenitor pool in Vegffl/fl telencephalon (G), whereas Ki67-positive progenitors spanned the entire pallium of VegfΔTie2-Cre embryos (H). Boxed regions were magnified; white asterisks indicate Ki67-negative CP (G), and yellow asterisks indicate abnormal proliferation (H). I, J, MAP2 immunoreactivity reveals normal neuronal differentiation zone of E15 Vegffl/fl pallium (I, white asterisks), which is reduced in VegfΔTie2-Cre pallium (J, yellow asterisks). A band of MAP2+ cells were abnormally positioned in the VegfΔTie2-Cre IZ-SVZ (J, yellow arrows). K, High-magnification image of MAP2+ neurons in the VegfΔTie2-Cre SVZ. L, M, Tbr1 immunoreactivity in E15 Vegffl/fl (L) and VegfΔTie2-Cre (M) pallium. Boxed regions are magnified. The uniform arrangement of CP cells in Vegffl/fl pallium (L, white asterisk) was altered in VegfΔTie2-Cre pallium with abnormally thicker (M, yellow arrow) and thinner (M, yellow asterisk) cell arrangement in the CP. N, O, RC2 immunostaining reveals continuous radial glial fibers extending from VZ to pial surface in Vegffl/fl pallium (N) and shortened radial glial processes in VegfΔTie2-Cre pallium (O, red asterisks). P, Q, GAD65/67 immunoreactivity shows decreased stream of GABA neurons in VegfΔTie2-Cre pallium extending to the medial edge (Q, yellow asterisks) compared with Vegffl/fl pallium (P, white asterisks). R–U, Calbindin+ and calretinin+ neurons were decreased (S, U, yellow asterisks) in E15 VegfΔTie2-Cre pallium compared with Vegffl/fl pallium (R, T, white asterisks). Calretinin immunoreactivity was decreased in the hippocampal primodium of VegfΔTie2-Cre embryos (U, yellow arrow). V, W, Uniform calretinin labeling in MZ and SP of Vegffl/fl embryos (V) and irregular calretinin labeling outside MZ in VegfΔTie2-Cre telencephalon (W). W, The boxed region was magnified, and yellow arrows point to calretinin+ cells outside MZ. X, Y, CHL1 immunostaining reveals axonal tracts in Vegffl/fl (X) and VegfΔTie2-Cre (Y) pallium. Y, Yellow arrow points to displaced axonal tract. DAPI (blue) was used to label nuclei (A, B, G–M, P–Y). Scale bars: A (applies also to B, E–J, L, M, P–U, X, Y), 100 μm; C (applies also to D), 75 μm; N (applies to O, V, W), 50 μm; magnified inset in G (applies to magnified insets in H, L, M), 50 μm; W (magnified inset), 25 μm. n = 8.

These defects in neuronal proliferation, differentiation, and lamination observed in E15 dorsal telencephalon of VegfΔTie2-Cre embryos became marked by E17 and reflected diversely in a wide range of severe cortical malformations (Fig. 4A–F). Ki67-positive proliferating progenitors were observed infiltrating the CP ectopias in E17 VegfΔTie2-Cre embryos (Fig. 4B). Breakdown of pial membrane with concurrent neuronal overmigration as well as disruption of the VZ continuity was particularly obvious (Fig. 4B). Abnormal crests and grooves of MAP2-positive cells revealed intense polymicrogyria in E17 VegfΔTie2-Cre telencephalon (Fig. 4D). Tbr1 immunostaining showed split CP with the two edges of the overextended CP misplaced into SVZ-IZ regions (Fig. 4F). These results reveal the crucial role of endothelial VEGF that impacts neuronal progenitors, neurons, and radial glia in the developing telencephalon.

Figure 4.
Figure 4.

Marked disturbance of neuronal development and axonal tracts in E17 VegfΔTie2-Cre embryos. A, B, Ki67 labeling of E17 Vegffl/fl (A) and VegfΔTie2-Cre (B) telencephalon. Boxed regions were magnified. A, Inset, White asterisks indicate Ki67-negative CP. B, Inset, Yellow arrows indicate abnormally proliferating Ki67-positive cells in a cortical ectopia. Red arrow points to a disrupted site along the ventricular surface. Pink arrow points to DAPI-labeled ectopia breaching pial surface. C, D, MAP2 labeling revealing normal differentiation zone in Vegffl/fl pallium (C) and abnormal differentiation in VegfΔTie2-Cre pallium (D, yellow arrows). E, F, Tbr1 immunoreactivity in Vegffl/fl (E) and VegfΔTie2-Cre (F) embryos. Boxed regions were magnified, revealing well-organized CP in Vegffl/fl embryos (E, white asterisk) and disrupted CP arrangement in VegfΔTie2-Cre embryo (F, yellow asterisks). G–Z, CHL1 labeling of axonal tracts in Vegffl/fl and VegfΔTie2-Cre embryos along the rostrocaudal axis. White arrows indicate well-formed axon tracts in sections from Vegffl/fl embryos (G, I, M, S, U, W, Y). Axon tracts were severely disturbed in corresponding sections from VegfΔTie2-Cre embryos (H, J, N, T, V, X, Z, follow yellow arrows) and extended into cortical plate (N, T, V, X, Z, yellow stars). Corpus callosum fibers cross the midline in Vegffl/fl embryos (K, white arrow) but stop abruptly at the midline in VegfΔTie2-Cre embryos (L, green arrow). The two limbs of the anterior commissure (ac) cross at the midline in Vegffl/fl embryos at two levels along the rostrocaudal axis (O, Q). Crossing of ac at the midline does not happen at the rostral level in VegfΔTie2-Cre embryos (P, green arrow), but only at the caudal level (R) indicative of a thinner commissural tract in VegfΔTie2-Cre embryos. n = 5.

Disturbed axonal tracts in VegfΔTie2-Cre embryos

Axonal fiber tracts containing cortical efferent and afferent fibers innervated the internal capsule in E17 VegfΔTie2-Cre embryos, and a loss of trajectory of axon tracts was not observed. However, axon tracts labeled with CHL1 were severely defective at all rostrocaudal levels in the E17 VegfΔTie2-Cre telencephalon (Fig. 4G–Z). In contrast to the normal location of subcortical axonal fiber tracts just beneath the CP in Vegffl/fl embryos, in VegfΔTie2-Cre embryos, the axon tracts thinned severely at places (Fig. 4H,N,T,V) or abnormally thickened and extended into CP (Fig. 4N,T,V,X,Z). Fiber tracts that decussate at the midline, most notably the corpus callosum and anterior commissure, were affected in VegfΔTie2-Cre embryos (Fig. 3L,P). Although corpus callosum fibers crossed the midline in Vegffl/fl embryos (Fig. 3K), the callosal projections failed to show decussation in VegfΔTie2-Cre embryos and stopped abruptly at the midline (Fig. 3L). Although the anterior commissure crossed at two levels along the rostrocaudal axis in Vegffl/fl embryos, the anterior commissure crossed at the midline only at the more caudal level, indicative of a thinner commissural tract in VegfΔTie2-Cre embryos (Fig. 3O–R). Thus, development of the major axon systems of the telencephalon was significantly impaired in VegfΔTie2-Cre embryos.

Discussion

Our results uncover endothelial VEGF's novel and critical role in molding the development of periventricular vessels, neuroepithelial cells, neurons, and eventually the telencephalic architecture. At early embryonic stages (E9–E13), neuronal VEGF signaling is the primary player regulating telencephalic development. By E15, when vessel density increases along with concurrent expansion of the cortex, endothelial VEGF becomes critical for ongoing developmental processes. Telencephalic angiogenesis was affected along with significant consequences for neuronal development. These results lay the basis for future studies investigating detailed mechanisms mediated by endothelial VEGF. It would also be interesting to investigate what different isoforms of endothelial VEGF are secreted and to what degree these isoforms specifically affect vascular and/or neuronal development.

The Tie2-Cre line used in this analysis has also been shown to be active in the hematopoietic system (Goossens et al., 2011), and previous reports have demonstrated that macrophage sources of VEGF as well as other angiogenic factors play important roles in vessel anastomosis during early vessel patterning in the developing CNS (Fantin et al., 2013). However, up until E13, we could not detect any changes in the vasculature associated with endothelial loss of VEGF and only by E15 were changes in vessel patterning observed. Microglia (the macrophage population of the CNS) increases significantly in number after birth concurrent with myelination events during postnatal brain development (Erblich et al., 2011). Despite loss of microglia, mouse embryonic forebrain development has been reported to be normal (Erblich et al., 2011). In addition, in the embryonic telencephalon, up until E17, microglia have been reported to be either absent from the cortical plate (Sturrock, 1981) or very sparsely distributed throughout the cortex (Cunningham et al., 2013). Together, our study signifies an important role for endothelial VEGF in promoting periventricular angiogenesis.

In addition, endothelial VEGF is capable of acting as a key “stop” signal for neuronal proliferation and migration in the embryonic telencephalon. In the absence of endothelial VEGF, overproduction of neuronal progenitors combined with excess neuronal migration in some cases and/or disrupted neuronal migration caused severe malformations in the embryonic telencephalon by late embryonic stages. These findings shed new light on the role of endothelial VEGF in regulating cortical lamination. Moreover, the phenotypic differences resulting from specifically deleting VEGF from neuronal progenitors versus endothelial cells becomes apparent. Although conditional deletion of Vegf from neuronal progenitors resulted in decreased neuronal proliferation, increased apoptosis, and extensive degeneration of the cerebral cortex (Haigh et al., 2003; Raab et al., 2004), in sharp contrast, conditional deletion of VEGF from endothelial cells resulted in an abnormal increase of neuronal proliferation and disturbed neuronal migration during cortical development. The overmigration of neurons past the breached pial membrane, radial glial detachment, and formation of MZ heterotopias in VegfΔTie2-Cre embryos resembles cobblestone lissencephaly (Olson and Walsh, 2002), one common form of cortical dyslamination seen in human congenital muscular dystrophy syndromes. In addition to the key radial migration event essential for cerebral cortex development (Rakic, 1988; Hatten, 2002), the tangential migration event of GABA neurons (Marin and Rubenstein, 2001) was also affected in VegfΔTie2-Cre embryos. Further work is needed to clarify whether this is a result of a loss of GABA neuron populations in the absence of endothelial VEGF or the lack of necessary guidance cues along the tangential route or both. Axonal tracts that follow stereotyped pathways (Lopez-Bendito and Molnar, 2003) were markedly impaired in VegfΔTie2-Cre embryos, bringing forth additional questions as to the precise role of endothelial VEGF in axon growth and guidance. Interestingly, neuronal VEGF cannot compensate for all of these defects caused in the absence of endothelial VEGF.

Lack of endothelial VEGF may contribute to diseases, such as cobblestone lissencephaly, mental retardation, epilepsy, and developmental dyslexia. Our data illustrate the intimate and symbiotic relationship between endothelial cells and neurons of the developing brain that respond meticulously and independently to VEGF signals depending on the cell type that secretes it.

Footnotes

  • This work was supported by a National Alliance for Research on Schizophrenia and Depression Young Investigator Award and National Institutes of Health Grants R21NS064386 and R01NS073635 to A.V.

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to Dr. Anju Vasudevan, Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience, McLean Hospital/Harvard Medical School, 115 Mill Street, Belmont, MA 02478. avasudevan{at}mclean.harvard.edu

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Endothelial VEGF Sculpts Cortical Cytoarchitecture
Suyan Li, Katharina Haigh, Jody J. Haigh, Anju Vasudevan
Journal of Neuroscience 11 September 2013, 33 (37) 14809-14815; DOI: 10.1523/JNEUROSCI.1368-13.2013
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