Figure 5. Adcy1 transcript rhythms in different retinal layers and whole retinas of WT, Npas2−/−, and Drd4−/− mice. A, In WT mice, Adcy1 transcripts are robustly rhythmic the in GCL (solid red; one-way ANOVA on ranks, H4 = 15.548, p = 0.004) and PRL (short-dashed red; one-way ANOVA, F(4,23) = 18.627, p < 0.001), but not in the INL (long-dashed red). B, Adcy1 transcripts are arrhythmic and stay at low levels in the GCL of Npas2−/− (green; one-way ANOVA, F(4,20) = 0.975, p = 0.443; two-way ANOVA, NPAS2 deficiency × time interaction, F(4,40) = 6.809, p < 0.001) and Drd4−/− mice (blue; one-way ANOVA, F(4,18) = 0.644, p = 0.638; two-way ANOVA, D4 receptor deficiency × time interaction, F(4,38) = 5.082, p = 0.002). C, In the PRL of Npas2−/− mice, the Adcy1 transcript expression (green) is rhythmic and not significantly different from WT (red) (one-way ANOVA, F(4,19) = 4.092, p = 0.015; two-way ANOVA, NPAS2 deficiency × time interaction, F(4,42) = 2.760, p = 0.055). In the PRL of Drd4−/− mice (blue; one-way ANOVA, F(4,18) = 0.896, p = 0.487; two-way ANOVA, D4R deficiency × time interaction, F(4,41) = 7.270, p < 0.001), the Adcy1 transcript expression is arrhythmic and stays at low levels. D, The rhythmic expression of Adcy1 transcripts in the whole retina is not significantly different between WT and Npas2−/− mice (p = 0.407, two-way ANOVA). Data are expressed as the mean ± SEM. n = 5–6 mice per time point.