Figure 1. Visualization of membrane trafficking dynamics in Drosophila. A, Schematic of intersecting trafficking pathways in synaptic boutons at the Drosophila NMJ. B, Super resolution imaging (photobleaching microscopy with nonlinear processing) (Munck et al., 2012) of a larval synaptic bouton expressing N- and C-Split Venus-GRP1-PH (green), a marker for PI(3,4,5)P3, and immunolabeled with anti-Syntaxin1A (red) and anti-Rim Binding Protein (RBP, blue) (Khuong et al., 2013). The images indicate that PI(3,4,5)P3 and Syntaxin1A cluster preferentially at active zones that are marked by RBP. Scale bar, 500 nm. Image courtesy of Thang Manh Khuong (KU Leuven, Leuven, Belgium). C, Live imaging reveals trafficking of the BMP receptor Tkv-mCherry in synaptic boutons (top), and dynamic movement along axon terminals (indicated in yellow as a kymograph; bottom).