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Journal Club

Is GABA Co-Released with Glutamate from Hippocampal Mossy Fiber Terminals?

Maddalena Delma Caiati
Journal of Neuroscience 30 January 2013, 33 (5) 1755-1756; https://doi.org/10.1523/JNEUROSCI.5019-12.2013
Maddalena Delma Caiati
International School for Advanced Studies (SISSA), Neuroscience Area, Trieste 34136, Italy
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The coexistence and co-release of different neurotransmitters from the same fiber has been well documented in several brain structures, including the retina, the spinal cord, and the auditory system (Hnasko and Edwards, 2012). Although mossy fibers (MFs), the axons of dentate gyrus granule cells, release glutamate and GABA early in postnatal development (Walker et al., 2001; Gutiérrez et al., 2003; Safiulina et al., 2006), they normally provide monosynaptic glutamatergic excitation and disynaptic GABAergic inhibition to the CA3 hippocampal field in adults. But in some conditions, such as kindling or activity-dependent processes, MFs can transiently resume a GABAergic phenotype in adulthood. This suggests that MFs can switch, in a developmentally and activity-dependent regulated way, the type of neurotransmitter released (Gutiérrez, 2005).

Studies using immunohistochemistry and electron microscopy have revealed that MFs possess the full machinery to synthesize, store, and release GABA. Glutamic acid decarboxylase (GAD67/65), the enzyme that catalyzes GABA synthesis, as well as GABA itself and the vesicular GABA transporter, VGAT, have all been detected within MF terminals. Importantly, immunogold experiments have demonstrated that AMPA and GABAA receptors are sometimes colocalized on postsynaptic sites in close apposition to MF terminals, strongly suggesting that MFs may convey a GABAergic signal to their targets (Gutiérrez, 2005). Consistent with this hypothesis, monosynaptic GABAergic currents have been recorded in CA3 principal cells upon granule cell stimulation in the dentate gyrus in acute hippocampal slices from newborn (Safiulina et al., 2006) or juvenile animals (Walker et al., 2001; Gutiérrez et al., 2003). The evoked responses fulfill the criteria for identification of MF inputs: strong paired pulse facilitation, short term frequency-dependent facilitation, and sensitivity to group II and III mGluR agonists (Safiulina et al., 2006).

The data discussed above have been challenged, however. Specifically, Uchigashima et al. (2007) questioned the GABAergic nature of MF-CA3 responses on the basis that, at least in young animals, the stimulation protocol generally used to activate MFs might coactivate adjacent GABAergic terminals, thus causing misinterpretation of the results. Given the complex nature of the neuronal network of the dentate gyrus and CA3 area, the possibility that interneurons with axonal or dendritic projections to the dentate gyrus could be activated with minimal stimulation protocol is plausible. This controversial issue may be solved by performing paired recordings from interconnected granule cells and CA3 principal neurons.

In a study recently published in The Journal of Neuroscience, Cabezas et al. (2012) used paired recordings of interconnected neurons in organotypic hippocampal slice cultures from GAD67–EGFP transgenic mice to explore the possibility that MFs can co-release glutamate and GABA onto CA3 principal cells. First, by performing immunocytochemical experiments from the hippocampus of postnatal day 15 (P15) old mice, the authors found that GAD67 is expressed only in a subset of MF terminals immunopositive for ZnT3, a selective MF marker. Interestingly, GAD67-positive granule cells showed signs of immaturity, including expression of doublecortin but not calbindin, low membrane capacitance, moderate input resistance, and small amplitude action potentials.

Next, in organotypic hippocampal slices prepared from P7–P8 old mice and kept in cultures for 10–12 days, Cabezas et al. (2012) used local photolysis of caged glutamate to examine postsynaptic responses evoked by photostimulation of individual, visually identified granule cells. Despite the expression of GAD67 in a subpopulation of immature granule cells, no unitary GABAA-mediated synaptic currents were detected in any of the 39 pairs of interconnected granule cells and CA3 principal cells examined, casting doubts on previous findings obtained from acute hippocampal slices (Walker et al., 2001; Gutiérrez et al., 2003; Safiulina et al., 2006). Based on their reversal potential and pharmacology, unitary currents were identified as mediated by AMPA/kainate receptors.

Notwithstanding the lack of GABA-mediated postsynaptic currents, however, Cabezas et al. (2012) showed that GAD67-positive granule cells release GABA. They demonstrated that GABA released from MF terminals acts upon presynaptic GABAB receptors, reducing MF excitability. Thus, repetitive activation of EGFP-positive neurons in the dentate gyrus (a train of 30 orthodromic action currents at 25 Hz elicited by depolarizing voltage pulses through the patch pipette) transiently reduced the probability of evoking antidromic action currents by extracellular stimulation of MFs in stratum lucidum, and this effect was blocked by selective GABAB receptor antagonists. Therefore, it appears that, in juvenile animals, a transient MF GABAergic phenotype is important for instructing presynaptic rather than postsynaptic elements of synapses.

Although the data presented by Cabezas et al. (2012) favor the hypothesis that monosynaptic GABAergic currents recorded from CA3 pyramidal cells in acute slices (upon stimulation of granule cells) originate from direct activation of hilar interneurons and not dentate gyrus granule cells, it is worth noting that Cabezas et al. (2012) used a different preparation than previous studies. Specifically, whereas earlier studies (Walker et al., 2001; Gutiérrez et al., 2003; Safiulina et al., 2006) used acute hippocampal slices, Cabezas et al. (2012) used organotypic hippocampal slices kept in cultures for 10–12 days before recording. Because it is impossible to determine the exact developmental stage of MFs in culture, making a direct comparison between the two preparations is extremely difficult. Furthermore, although organotypic hippocampal slices maintain some local circuitry intact, they develop in isolation and hence they lack the experience-dependent plasticity characteristic of behaving animals. Because MF-dependent GABAergic transmission is strongly activity dependent (Gutierrez et al., 2003), the lack of extrinsic afferents in slice cultures may interfere with the acquisition of a functional GABAergic phenotype from granule cells. In addition, compared to acute slices, organotypic cultures exhibit enhanced glutamatergic connectivity as demonstrated by the four- to five-fold increase in the frequency of glutamatergic but not GABAergic miniature postsynaptic currents (De Simoni et al., 2003). Moreover, the fact that astrocytes do not reach a full maturation in organotypic cultures (Derouiche, 1993) might alter the normal glutamate and GABA metabolism, possibly lowering the level of GABA released from MF terminals. Finally, it is unclear whether, GABAA receptors facing MF terminals remain present and functional in organotypic cultures.

In addition to the caveats listed above, the observations of Cabezas et al. (2012) are difficult to reconcile with a recent report by Beltran and Gutiérrez (2012), which examined synaptic responses evoked by stimulation of single identified MF boutons attached to the apical dendrites of mechanically isolated pyramidal cells. Such stimulation produced synaptic currents that, like typical MF-evoked responses, showed a high degree of facilitation upon repetitive stimulation and were blocked by group II mGluR agonists. Interestingly, whereas stimulation of MF boutons in neurons dissociated from adult animals generated exclusively glutamate receptor-mediated responses, stimulation of MF boutons from younger animals produced either GABAergic or mixed GABAergic and glutamatergic responses.

In summary, the question of whether activation of immature MF terminals elicits monosynaptic GABAergic responses in CA3 pyramidal cells remains unsolved. Recently developed tools may help to clarify this issue. For example, expressing channelrhodopsin-2 (ChR2) via a retroviral vector in hippocampal granule cell progenitors would allow one to selectively activate ChR2-positive granule cells by photostimulation and to identify the nature of the neurotransmitter released by MFs onto patched CA3 principal cells. Classical criteria for MF identification could then be used to ascertain that ChR2-driven synaptic responses were truly MFs.

Alternatively, by sequentially uncaging glutamate with the beam-multiplexed two-photon laser, a novel optical method developed by Nikolenko et al. (2007), it would be possible to selectively activate (in acute hippocampal slices from GAD67-EGFP transgenic mice) up to a thousand GAD67-positive granule cells, causing them to fire. This would allow investigation of the nature of monosynaptic-evoked responses in CA3-targeted pyramidal cells.

Either of these approaches would permit one to selectively activate granule cells, as well as to overcome the difficulty of finding connected pairs of neurons. Such experiments might finally elucidate the role of GABA in MF terminals.

Footnotes

  • Editor's Note: These short, critical reviews of recent papers in the Journal, written exclusively by graduate students or postdoctoral fellows, are intended to summarize the important findings of the paper and provide additional insight and commentary. For more information of the format and purpose of the Journal Club, please see http://www.jneurosci.org/misc/ifa_features.shtml.

  • I acknowledge Prof. Enrico Cherubini for his helpful review of the manuscript.

  • Correspondence should be addressed to Maddalena Delma Caiati, SISSA, Via Bonomea 265, Trieste (TS) 34136, Italy. caiati{at}sissa.it

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The Journal of Neuroscience: 33 (5)
Journal of Neuroscience
Vol. 33, Issue 5
30 Jan 2013
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Is GABA Co-Released with Glutamate from Hippocampal Mossy Fiber Terminals?
Maddalena Delma Caiati
Journal of Neuroscience 30 January 2013, 33 (5) 1755-1756; DOI: 10.1523/JNEUROSCI.5019-12.2013

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Is GABA Co-Released with Glutamate from Hippocampal Mossy Fiber Terminals?
Maddalena Delma Caiati
Journal of Neuroscience 30 January 2013, 33 (5) 1755-1756; DOI: 10.1523/JNEUROSCI.5019-12.2013
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  • Author Response to Caiati Journal Club
    Dr. Jean-Christophe Poncer and Dr. Carolina Cabezas
    Published on: 26 July 2017
  • Published on: (26 July 2017)
    Page navigation anchor for Author Response to Caiati Journal Club
    Author Response to Caiati Journal Club
    • Dr. Jean-Christophe Poncer, Author, INSERM
    • Other Contributors:
      • Dr. Carolina Cabezas

    A response to this article may be found here.

    Competing Interests: None declared.

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