Skip to main content

Umbrella menu

  • SfN.org
  • eNeuro
  • The Journal of Neuroscience
  • Neuronline
  • BrainFacts.org

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
  • ALERTS
  • FOR AUTHORS
    • Preparing a Manuscript
    • Submission Guidelines
    • Fees
    • Journal Club
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE
  • SfN.org
  • eNeuro
  • The Journal of Neuroscience
  • Neuronline
  • BrainFacts.org

User menu

  • Log in
  • Subscribe
  • My alerts

Search

  • Advanced search
Journal of Neuroscience
  • Log in
  • Subscribe
  • My alerts
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
  • ALERTS
  • FOR AUTHORS
    • Preparing a Manuscript
    • Submission Guidelines
    • Fees
    • Journal Club
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE
PreviousNext
Articles, Cellular/Molecular

Storage and Uptake of d-Serine into Astrocytic Synaptic-Like Vesicles Specify Gliotransmission

Magalie Martineau, Ting Shi, Julien Puyal, Ann M. Knolhoff, Jérôme Dulong, Bruno Gasnier, Jürgen Klingauf, Jonathan V. Sweedler, Reinhard Jahn and Jean-Pierre Mothet
Journal of Neuroscience 20 February 2013, 33 (8) 3413-3423; DOI: https://doi.org/10.1523/JNEUROSCI.3497-12.2013
Magalie Martineau
1Neurocentre Magendie, National Institute of Health and Medical Research, Unit 862 and
2University of Bordeaux, F-33077 Bordeaux, France,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ting Shi
3Department of Chemistry and Neuroscience Program, University of Illinois, Urbana, Illinois 61801,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julien Puyal
4Department of Fundamental Neurosciences, University of Lausanne, CH-1005 Lausanne, Switzerland,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ann M. Knolhoff
3Department of Chemistry and Neuroscience Program, University of Illinois, Urbana, Illinois 61801,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jérôme Dulong
1Neurocentre Magendie, National Institute of Health and Medical Research, Unit 862 and
2University of Bordeaux, F-33077 Bordeaux, France,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bruno Gasnier
5University Paris Descartes, Sorbonne Paris Cité, National Center for Scientific Research, Joint Research Unit 8192, F-75006 Paris, France,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jürgen Klingauf
6Institute of Medical Physics and Biophysics, University of Muenster, D-48149 Muenster, Germany,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan V. Sweedler
3Department of Chemistry and Neuroscience Program, University of Illinois, Urbana, Illinois 61801,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Reinhard Jahn
7Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D-37077 Goettingen, Germany, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jean-Pierre Mothet
1Neurocentre Magendie, National Institute of Health and Medical Research, Unit 862 and
2University of Bordeaux, F-33077 Bordeaux, France,
8Aix-Marseille University, National Center for Scientific Research, CRN2M, Joint Research Unit 7286, F-13344 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Glial cells are increasingly recognized as active players that profoundly influence neuronal synaptic transmission by specialized signaling pathways. In particular, astrocytes have been shown recently to release small molecules, such as the amino acids l-glutamate and d-serine as “gliotransmitters,” which directly control the efficacy of adjacent synapses. However, it is still controversial whether gliotransmitters are released from a cytosolic pool or by Ca2+-dependent exocytosis from secretory vesicles, i.e., by a mechanism similar to the release of synaptic vesicles in synapses. Here we report that rat cortical astrocytes contain storage vesicles that display morphological and biochemical features similar to neuronal synaptic vesicles. These vesicles share some, but not all, membrane proteins with synaptic vesicles, including the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) synaptobrevin 2, and contain both l-glutamate and d-serine. Furthermore, they show uptake of l-glutamate and d-serine that is driven by a proton electrochemical gradient. d-Serine uptake is associated with vesicle acidification and is dependent on chloride. Whereas l-serine is not transported, serine racemase, the synthesizing enzyme for d-serine, is anchored to the membrane of the vesicles, allowing local generation of d-serine. Finally, we reveal a previously unexpected mutual vesicular synergy between d-serine and l-glutamate filling in glia vesicles. We conclude that astrocytes contain vesicles capable of storing and releasing d-serine, l-glutamate, and most likely other neuromodulators in an activity-dependent manner.

View Full Text
Back to top

In this issue

The Journal of Neuroscience: 33 (8)
Journal of Neuroscience
Vol. 33, Issue 8
20 Feb 2013
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Advertising (PDF)
  • Ed Board (PDF)
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Storage and Uptake of d-Serine into Astrocytic Synaptic-Like Vesicles Specify Gliotransmission
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Citation Tools
Storage and Uptake of d-Serine into Astrocytic Synaptic-Like Vesicles Specify Gliotransmission
Magalie Martineau, Ting Shi, Julien Puyal, Ann M. Knolhoff, Jérôme Dulong, Bruno Gasnier, Jürgen Klingauf, Jonathan V. Sweedler, Reinhard Jahn, Jean-Pierre Mothet
Journal of Neuroscience 20 February 2013, 33 (8) 3413-3423; DOI: 10.1523/JNEUROSCI.3497-12.2013

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
Storage and Uptake of d-Serine into Astrocytic Synaptic-Like Vesicles Specify Gliotransmission
Magalie Martineau, Ting Shi, Julien Puyal, Ann M. Knolhoff, Jérôme Dulong, Bruno Gasnier, Jürgen Klingauf, Jonathan V. Sweedler, Reinhard Jahn, Jean-Pierre Mothet
Journal of Neuroscience 20 February 2013, 33 (8) 3413-3423; DOI: 10.1523/JNEUROSCI.3497-12.2013
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

Articles

  • Choice Behavior Guided by Learned, But Not Innate, Taste Aversion Recruits the Orbitofrontal Cortex
  • Maturation of Spontaneous Firing Properties after Hearing Onset in Rat Auditory Nerve Fibers: Spontaneous Rates, Refractoriness, and Interfiber Correlations
  • Insulin Treatment Prevents Neuroinflammation and Neuronal Injury with Restored Neurobehavioral Function in Models of HIV/AIDS Neurodegeneration
Show more Articles

Cellular/Molecular

  • The Cellular Electrophysiological Properties Underlying Multiplexed Coding in Purkinje Cells
  • Phase-locking requires efficient Ca2+ extrusion at the auditory hair cell ribbon synapses
  • Dopamine Transporter Localization in Medial Forebrain Bundle Axons Indicates Its Long-Range Transport Primarily by Membrane Diffusion with a Limited Contribution of Vesicular Traffic on Retromer-Positive Compartments
Show more Cellular/Molecular
  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
  • Feedback
(JNeurosci logo)
(SfN logo)

Copyright © 2021 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.