Article Information
- Received July 22, 2012
- Revision received December 11, 2012
- Accepted December 21, 2012
- First published February 20, 2013.
- Version of record published February 20, 2013.
Author Information
- Magalie Martineau1,2,
- Ting Shi3,
- Julien Puyal4,
- Ann M. Knolhoff3,
- Jérôme Dulong1,2,
- Bruno Gasnier5,
- Jürgen Klingauf6,
- Jonathan V. Sweedler3,
- Reinhard Jahn7, and
- Jean-Pierre Mothet1,2,8
- 1Neurocentre Magendie, National Institute of Health and Medical Research, Unit 862 and
- 2University of Bordeaux, F-33077 Bordeaux, France,
- 3Department of Chemistry and Neuroscience Program, University of Illinois, Urbana, Illinois 61801,
- 4Department of Fundamental Neurosciences, University of Lausanne, CH-1005 Lausanne, Switzerland,
- 5University Paris Descartes, Sorbonne Paris Cité, National Center for Scientific Research, Joint Research Unit 8192, F-75006 Paris, France,
- 6Institute of Medical Physics and Biophysics, University of Muenster, D-48149 Muenster, Germany,
- 7Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D-37077 Goettingen, Germany, and
- 8Aix-Marseille University, National Center for Scientific Research, CRN2M, Joint Research Unit 7286, F-13344 Marseille, France
Author contributions
Author contributions: M.M., B.G., J.V.S., R.J., and J.-P.M. designed research; M.M., T.S., J.P., A.M.K., J.D., and J.-P.M. performed research; J.K. and R.J. contributed unpublished reagents/analytic tools; M.M., T.S., J.P., A.M.K., J.V.S., and J.-P.M. analyzed data; M.M., R.J., and J.-P.M. wrote the paper.
Disclosures
- Received July 22, 2012.
- Revision received December 11, 2012.
- Accepted December 21, 2012.
This work was supported by grants from the National Center for Scientific Research (J.P.M., B.G.), National Institute of Health and Medical Research (J.P.M.), Regional Council of Aquitaine (J.P.M.), National Research Agency (J.P.M.), and Swiss National Science Foundation (J.P.), National Science Foundation Grants CHE-1111705 and CHE0526692 (J.V.S.), and National Institute of Health Grant P30DA018310 (J.V.S.). M.M. is a recipient of a PhD fellowship from the National Ministry of Education, Research, and Technology, from the Foundation of Medical Research, and was granted by the European Molecular Biology Organisation for a short-term fellowship. The monoclonal antibodies against Na+/K+-ATPase (α6F) developed by D. M. Fambrough and against SV2 developed by K. M. Buckley were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the University of Iowa, Department of Biological Sciences (Iowa City, IA). We are grateful to Dionysia T. Theodosis for her technical help in electron microscopy analysis of purified vesicles, to Rosina Giordano for launching preliminary radioactivity uptake experiments, to Peter Nemes for his assistance with CE–MS, and to Stéphane H. R. Oliet for his continuous support. We thank Shigeo Takamori and Paola Bezzi for their valuable comments and suggestions. We are grateful to Thierry Galli, Nicolas Morel, and Herman Wolosker for generously providing antibodies and to Silvia Sacchi and Loredano Pollegioni for the generous gift of RgDAAO and Bsglycine oxidase. We thank the Photonic Imaging team of the Bordeaux Imaging Center for its technical assistance and expertise and the Center of Electron Microscopy at the University of Lausanne for the use of their electron microscopes. We thank one of the reviewers for a valuable experimental suggestion.
- Correspondence should be addressed to either of the following at their present addresses: Dr. Jean-Pierre Mothet, Aix-Marseille University, National Center for Scientific Research, CRN2M, Joint Research Unit 7286, F-13344 Marseille, France, jean-pierre.mothet{at}univ-amu.fr; or Dr. Magalie Martineau, Institute of Medical Physics and Biophysics, University of Muenster, D-48149 Muenster, Germany, magmart{at}uni-muenster.de
