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Featured ArticleArticles, Neurobiology of Disease

The Angelman Syndrome Protein Ube3a/E6AP Is Required for Golgi Acidification and Surface Protein Sialylation

Kathryn H. Condon, Jianghai Ho, Camenzind G. Robinson, Cyril Hanus and Michael D. Ehlers
Journal of Neuroscience 27 February 2013, 33 (9) 3799-3814; DOI: https://doi.org/10.1523/JNEUROSCI.1930-11.2013
Kathryn H. Condon
1Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, and
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Jianghai Ho
1Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, and
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Camenzind G. Robinson
1Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, and
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Cyril Hanus
1Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, and
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Michael D. Ehlers
1Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, and
2Pfizer Worldwide Research and Development, Neuroscience Research Unit, Cambridge, Massachusetts 02139
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.1930-11.2013
PubMed 
23447592
Published By 
Society for Neuroscience
History 
  • Received April 13, 2011
  • Revision received November 30, 2012
  • Accepted December 22, 2012
  • First published February 27, 2013.
  • Version of record published February 27, 2013.
Copyright & Usage 
Copyright © 2013 the authors 0270-6474/13/333799-16$15.00/0

Author Information

  1. Kathryn H. Condon1,
  2. Jianghai Ho1,
  3. Camenzind G. Robinson1,
  4. Cyril Hanus1, and
  5. Michael D. Ehlers1,2
  1. 1Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, and
  2. 2Pfizer Worldwide Research and Development, Neuroscience Research Unit, Cambridge, Massachusetts 02139
  • C. G. Robinson's present address is United States Army Medical Research Institute of Infectious Diseases, Pathology Division, Fort Detrick, MD 21702.

  • C. Hanus' present address is Max Planck Institute for Brain Research, Deutschordenstrasse 46, 60528 Frankfurt, Germany.

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Author contributions

  1. Author contributions: K.H.C., J.H., C.G.R., C.H., and M.D.E. designed research; K.H.C., J.H., C.G.R., and C.H. performed research; K.H.C., J.H., C.G.R., and C.H. contributed unpublished reagents/analytic tools; K.H.C., J.H., C.G.R., and C.H. analyzed data; K.H.C. and M.D.E. wrote the paper.

  • C. G. Robinson's present address is United States Army Medical Research Institute of Infectious Diseases, Pathology Division, Fort Detrick, MD 21702.

  • C. Hanus' present address is Max Planck Institute for Brain Research, Deutschordenstrasse 46, 60528 Frankfurt, Germany.

Disclosures

    • Received April 13, 2011.
    • Revision received November 30, 2012.
    • Accepted December 22, 2012.
  • This work was supported by National Institutes of Health Grants NS047574 and MH064748, the Angelman Syndrome Foundation, and the Howard Hughes Medical Institute (M.D.E.). K.H.C. was supported by a fellowship from the Ruth K. Broad Foundation and a National Institutes of Health training grant to the Duke University Department of Neurobiology. We thank John Allen, Tom Helton, Ben Philpot, Joel Schwartz, and Koji Yashiro for helpful discussions and assistance; Richard Weinberg for assistance with electron microscopy; Marguerita Klein for generation of the pGolgi-CFP-YFP construct; Kewa Mou for assistance with embryonic mouse cultures; and John Allen, Juliet Hernandez, Angela Mabb, Tingting Wang, and Jason Yi for insightful discussions and critical comments on the manuscript.

  • M.D.E. is an employee and shareholder of Pfizer, Inc. The remaining authors declare no competing financial interests.

  • Correspondence should be addressed to Dr. Michael D. Ehlers, Neuroscience Research Unit, Pfizer, Inc., 700 Main Street, Cambridge, MA 02139. michael.ehlers{at}pfizer.com

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Jan 201812317
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Apr 201823517
May 201826304
Jun 20182345
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Apr 2019142615
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Feb 202042610
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May 202062536
Jun 202021512
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Aug 202032719
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Total 202084307195
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Apr 2021212316
May 2021124326
Jun 20214239
Jul 202132117
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Total 202195293224
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Jan 202353212
Feb 202341918
Mar 202332322
Apr 202362120
May 202341551
Jun 202311510
Jul 202372932
Aug 202321310
Sep 202341211
Total 202336179186
Total42316431072
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The Journal of Neuroscience: 33 (9)
Journal of Neuroscience
Vol. 33, Issue 9
27 Feb 2013
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The Angelman Syndrome Protein Ube3a/E6AP Is Required for Golgi Acidification and Surface Protein Sialylation
Kathryn H. Condon, Jianghai Ho, Camenzind G. Robinson, Cyril Hanus, Michael D. Ehlers
Journal of Neuroscience 27 February 2013, 33 (9) 3799-3814; DOI: 10.1523/JNEUROSCI.1930-11.2013

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The Angelman Syndrome Protein Ube3a/E6AP Is Required for Golgi Acidification and Surface Protein Sialylation
Kathryn H. Condon, Jianghai Ho, Camenzind G. Robinson, Cyril Hanus, Michael D. Ehlers
Journal of Neuroscience 27 February 2013, 33 (9) 3799-3814; DOI: 10.1523/JNEUROSCI.1930-11.2013
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