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Articles, Behavioral/Cognitive

Decreased Anxiety-Like Behavior and Gαq/11-Dependent Responses in the Amygdala of Mice Lacking TRPC4 Channels

Antonio Riccio, Yan Li, Evgeny Tsvetkov, Svetlana Gapon, Gui Lan Yao, Kiersten S. Smith, Elif Engin, Uwe Rudolph, Vadim Y. Bolshakov and David E. Clapham
Journal of Neuroscience 5 March 2014, 34 (10) 3653-3667; DOI: https://doi.org/10.1523/JNEUROSCI.2274-13.2014
Antonio Riccio
1Howard Hughes Medical Institute, Department of Cardiology, Boston Children's Hospital, Boston, Massachusetts 02115,
2Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115,
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Yan Li
3Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, and
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Evgeny Tsvetkov
3Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, and
4Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg 194223, Russia
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Svetlana Gapon
1Howard Hughes Medical Institute, Department of Cardiology, Boston Children's Hospital, Boston, Massachusetts 02115,
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Gui Lan Yao
2Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115,
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Kiersten S. Smith
3Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, and
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Elif Engin
3Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, and
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Uwe Rudolph
3Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, and
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Vadim Y. Bolshakov
3Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, and
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David E. Clapham
1Howard Hughes Medical Institute, Department of Cardiology, Boston Children's Hospital, Boston, Massachusetts 02115,
2Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115,
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Abstract

Transient receptor potential (TRP) channels are abundant in the brain where they regulate transmission of sensory signals. The expression patterns of different TRPC subunits (TRPC1, 4, and 5) are consistent with their potential role in fear-related behaviors. Accordingly, we found recently that mutant mice lacking a specific TRP channel subunit, TRPC5, exhibited decreased innate fear responses. Both TRPC5 and another member of the same subfamily, TRPC4, form heteromeric complexes with the TRPC1 subunit (TRPC1/5 and TRPC1/4, respectively). As TRP channels with specific subunit compositions may have different functional properties, we hypothesized that fear-related behaviors could be differentially controlled by TRPCs with distinct subunit arrangements. In this study, we focused on the analysis of mutant mice lacking the TRPC4 subunit, which, as we confirmed in experiments on control mice, is expressed in brain areas implicated in the control of fear and anxiety. In behavioral experiments, we found that constitutive ablation of TRPC4 was associated with diminished anxiety levels (innate fear). Furthermore, knockdown of TRPC4 protein in the lateral amygdala via lentiviral-mediated gene delivery of RNAi mimicked the behavioral phenotype of constitutive TRPC4-null (TRPC4−/−) mouse. Recordings in brain slices demonstrated that these behavioral modifications could stem from the lack of TRPC4 potentiation in neurons in the lateral nucleus of the amygdala through two Gαq/11 protein-coupled signaling pathways, activated via Group I metabotropic glutamate receptors and cholecystokinin 2 receptors, respectively. Thus, TRPC4 and the structurally and functionally related subunit, TRPC5, may both contribute to the mechanisms underlying regulation of innate fear responses.

  • amygdala
  • anxiety
  • cholecystokinin 4
  • fear
  • TRP channel
  • TRPC4
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The Journal of Neuroscience: 34 (10)
Journal of Neuroscience
Vol. 34, Issue 10
5 Mar 2014
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Decreased Anxiety-Like Behavior and Gαq/11-Dependent Responses in the Amygdala of Mice Lacking TRPC4 Channels
Antonio Riccio, Yan Li, Evgeny Tsvetkov, Svetlana Gapon, Gui Lan Yao, Kiersten S. Smith, Elif Engin, Uwe Rudolph, Vadim Y. Bolshakov, David E. Clapham
Journal of Neuroscience 5 March 2014, 34 (10) 3653-3667; DOI: 10.1523/JNEUROSCI.2274-13.2014

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Decreased Anxiety-Like Behavior and Gαq/11-Dependent Responses in the Amygdala of Mice Lacking TRPC4 Channels
Antonio Riccio, Yan Li, Evgeny Tsvetkov, Svetlana Gapon, Gui Lan Yao, Kiersten S. Smith, Elif Engin, Uwe Rudolph, Vadim Y. Bolshakov, David E. Clapham
Journal of Neuroscience 5 March 2014, 34 (10) 3653-3667; DOI: 10.1523/JNEUROSCI.2274-13.2014
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Keywords

  • amygdala
  • anxiety
  • cholecystokinin 4
  • fear
  • TRP channel
  • TRPC4

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