Figure 7. Loss of mTORC2 had less impact on oligodendrocyte development than loss of mTORC1. Representative Western blot (A) and quantification (B) of PLP, MBP, MOG, CNP, and MAG protein from P14 (B, left graph) and P29 (B, right graph) lysates isolated from whole spinal cord lysates of floxed Rictor (black) and Rictor cKO (red) animals. C, qPCR analysis RNA from whole spinal cord lysates from P14 and P29 floxed Rictor (black) and Rictor cKO (red) animals for myelin RNAs. D, Representative immunohistochemically stained sections (Olig2, blue; NG2, red; left) and quantification (right graphs) of cells in the dorsal columns of P14 Rictorfl/fl and Rictor cKO animals. The left graph represents the total number of Olig2-, NG2-, or CC1-positive cells. The right graph is the percentage of the total Olig2-positive cells that double labeled for NG2 or CC1. Since a small number of NG2-positive cells did not also express Olig2, they were not included in this quantification. E, Representative immunohistochemically stained sections (Olig2, red; CC1, green; left) and quantification (right graphs) of mature oligodendrocytes in the dorsal columns of P29 Rictorfl/fl and Rictor cKO spinal cord. The left graph represents the total number of Olig2-positive or CC1-positive cells. The right graph is the percentage of the total Olig2-positive cells that double labeled for CC1. Since a small number of CC1-positive cells did not also express Olig2, they were not included in this quantification. Scale bars, 75 μm. Values are displayed as ±SEM (n ≥ 3, *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001).