Abstract
Axonal degeneration represents an early pathological event in neurodegeneration, constituting an important target for neuroprotection. Regardless of the initial injury, which could be toxic, mechanical, metabolic, or genetic, degeneration of axons shares a common mechanism involving mitochondrial dysfunction and production of reactive oxygen species. Critical steps in this degenerative process are still unknown. Here we show that calcium release from the axonal endoplasmic reticulum (ER) through ryanodine and IP3 channels activates the mitochondrial permeability transition pore and contributes to axonal degeneration triggered by both mechanical and toxic insults in ex vivo and in vitro mouse and rat model systems. These data reveal a critical and early ER-dependent step during axonal degeneration, providing novel targets for axonal protection in neurodegenerative conditions.
Introduction
Axonal degeneration is a common and early feature of several neurodegenerative conditions leading to functional impairment; therefore, it constitutes an important target for neuroprotection. Degeneration of axons can be triggered by toxic, mechanic, ischemic, and others types of insults in both central and peripheral nervous systems (CNS and PNS, respectively) and is conserved in different phyla (Coleman and Freeman, 2010), suggesting a preserved mechanism for the loss of neuronal extensions. Degeneration of axons triggered by nerve injury, known as Wallerian degeneration (Waller, 1850), has been used widely as a model to study the mechanisms associated with axon loss or degeneration in other neurodegenerative conditions (Coleman and Freeman, 2010).
It was suggested that axonal mitochondria represent a convergence point for several pro-degenerative stimuli leading to axonal degeneration (Court and Coleman, 2012). Specifically, the opening of the mitochondrial permeability transition pore (mPTP) is a key event during axonal degeneration, because mPTP inhibition strongly delays Wallerian degeneration (Barrientos et al., 2011) and possibly also contributes to axonal degeneration during neurodegenerative conditions, including multiple sclerosis, Alzheimer's disease, ALS, and cerebral ischemia (Schinzel et al., 2005; Forte et al., 2007; Du et al., 2008; Martin et al., 2009). mPTP opening is triggered by mitochondrial calcium overloading (Chappell and Crofts, 1965; Crompton and Costi, 1988) and oxidative stress (Broekemeier et al., 1992). Consistent with this mechanism of opening, systemic antioxidant treatment and upregulation of endogenous antioxidant mechanisms protect axons from degeneration (Calixto et al., 2012).
In addition to oxidative stress, an increase in mitochondrial calcium is fundamental for mPTP opening (Bernardi, 2013), and it is known that axotomy increases calcium in the axon above the normal range (Ziv and Spira, 1995). Moreover, filling the axon with calcium using ionophores can destroy the axon within a few hours (George et al., 1995), and extracellular calcium chelation prevents this degeneration (Stys et al., 1990). The current view is that the increase in axonal calcium from the extracellular milieu after a degenerative stimulus, including axotomy and blockage of axonal transport, activates calpains, contributing to the last steps of axonal disintegration. Nevertheless, after an ischemic insult, early inhibition of calcium release from internal stores inhibited the loss of axonal function (Stys, 2005), suggesting that more than one source of axonal calcium might be involved at different stages of the axonal degenerative program.
The endoplasmic reticulum (ER) is one the main intracellular compartments for calcium storage (Pozzan et al., 1994). In peripheral axons, morphological evidences demonstrate that the ER forms a continuous network and establishes a close interaction with mitochondria (Tsukita and Ishikawa, 1976). Importantly, ER stress and calcium release from this organelle can induce mPTP opening in other cell types (Deniaud et al., 2008). Nevertheless, the role of the axonal ER in physiological and degenerative process in the axonal compartment is not fully understood.
Here we show that calcium release from axonal ER stores is involved in degeneration of axons triggered by mechanical injury and toxic insults. ER-derived calcium from ryanodine (RyaR) and IP3 (IP3R) receptors activates the mPTP and contributes to axonal degeneration in both cultured neurons and ex vivo nerve preparations. This is the first report to demonstrate the relevance of an ER-dependent calcium release in axonal degeneration and suggests that proper functioning of the ER in the axonal compartment is required for axonal viability.
Materials and Methods
Animals
Pregnant Sprague Dawley rats (150 g) and C57BL/6J mice (11 weeks old, 20–25 g) strain of either sex were obtained from the P. Catholic University animal house. Experiments with animals followed protocols approved by the Institutional Animal Care and Use Committees and complied with National Institutes of Health guidelines.
Nerve explant cultures
To study axonal degeneration ex vivo, 5 mm sciatic nerve segments were dissected from adult mice and cultured in 24-well dishes containing 400 μl of Neurobasal medium (Invitrogen), 2% B27 (Invitrogen), 0.3% l-glutamine, and 1% streptomycin/penicillin. Explants were cultured at 37°C and 5% CO2 for up to 3 d, with daily changes of media supplemented with drug or corresponding vehicle as control. In previous studies, these nerve explants required a drug concentration at least one order of magnitude higher than dissociated cultures to obtain similar effects (Court et al., 2009; Barrientos et al., 2011). This is probably because of the presence of the hematoneural barrier of the nerve (epineurium, perineurium, and collagen and extracellular matrix of the endoneurium) and myelinating Schwann cells. The following drugs were used: cyclosporine A (CsA; catalog #30024; Sigma Aldrich), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox; catalog #648471; Merck), 3,8-phenanthridinediamine (Dihydroethydium; catalog #D1168; Invitrogen), EGTA (catalog #E3889; Sigma-Aldrich), 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM; catalog #B6769; Invitrogen), Rya (catalog #559276; Calbiochem), ruthenium (Ru360; catalog #557440; Calbiochem), U73122 (1-[6[[(17β)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione) (catalog #U6756; Sigma), and caffeine (catalog #C0750; Sigma).
Dorsal root ganglion cultures
Briefly, E16 rat embryos were decapitated, and the vertebral column was removed. The spinal cord with dorsal root ganglia (DRGs) was dissected and placed in a Petri dish containing L-15 medium. For DRG explants, complete DRGs were cultured in 24-well dishes containing 400 μl of Neurobasal medium (Invitrogen), 2% B27 (Invitrogen), 0.3% l-glutamine, 1% streptomycin/penicillin, 4 μm aphidicolin, 7.5 μg/ml 5-fluoro-2- deoxyuridine, and 50 ng/ml NGF. The mixture of aphidicolin and fluoro-2-deoxyuridine inhibits proliferation of Schwann cells by inhibition of DNA polymerase (Spadari et al., 1985; Wallace and Johnson, 1989), and DRGs cultivated in these conditions seldom contain Schwann cells (Heermann et al., 2012). DRGs were cultured for 7 d at 37°C and 5% CO2. At day 7, ganglia were excised using a micropipette tip. This procedure eliminated all neuronal somas from the ganglia. For dissociated culture, DRGs were freed from the connective tissue sheaths and incubated with 2.0 mg/ml trypsin for 30 min at 37°C. After trypsinization, cells were gently triturated with fire-polished Pasteur pipettes in serum containing media and then placed in poly-d-lysine (0.1 mg/ml)-coated 35 mm culture plates and incubated at 37°C with 95% relative humidity and 5% CO2. The degeneration index was based on the ratio of the areas of fragmented axons versus total axonal area (Araki et al., 2004; Sasaki et al., 2009). Degenerated axon fragments were detected using the particle analyzer algorithm of NIH ImageJ, and the total fragmented axon area versus total axonal area was used to estimate a degeneration index.
Immunofluorescence and electron microscopy of sciatic nerves
For immunofluorescence analyses, sciatic nerves were fixed by immersion in 4% paraformaldehyde in 0.1 m PBS (1× PBS, pH 7.4) for 1 h, followed by three 10 min washes in 1× PBS, sucrose gradient (5, 10, and 20% in 1× PBS), and then embedded in OCT (catalog #25608-930; Sakura Finetek). Cryostat sections from the middle of the explants were cut transversely at 10 μm thickness and mounted on Superfrost Plus slides (catalog #4951PLUS; Thermo Fisher Scientific). Sections were washed in 1× PBS for 10 min and then blocked/permeabilized in 0.1% Triton X-100, 2% gelatin from cold-water fish (catalog #G7765; Sigma-Aldrich) in 1× PBS for 1 h at room temperature (RT). Sections were incubated in primary antibodies in blocking/permeabilizing solution overnight at 4°C, washed in 1× PBS three times for 10 min, and incubated in secondary antibodies for 2 h at RT. Sections were washed three times for 10 min in 1× PBS and mounted in Vectashield (Vector Laboratories). The number of axons per area of nerve tissue was assessed in images of neurofilament-immunostained explant sections using the particle analysis macro of NIH ImageJ. The following antibodies were used for immunofluorescence: rabbit anti-neurofilament heavy chain (NFH; catalog #N4142; Sigma-Aldrich) at 1:1000; donkey anti-rabbit TRITC (catalog #711-025-152; Jackson ImmunoResearch) at 1:300; and goat anti-chicken FITC (catalog #A-21449; Invitrogen) at 1:300.
For electron microscopy (EM) analyses, nerves were fixed overnight by immersion in 2.5% glutaraldehyde, 0.01% picric acid, and 0.1 m cacodylate buffer, pH 7.4. Nerves were rinsed in the same buffer, immersed in 1% OsO4 for 1 h, followed by en bloc incubation with 2% uranyl acetate for 2 h. Nerves were dehydrated with a graded series of ethanol and propylene oxide and infiltrated with Epon (catalog #18010; Ted Pella). Ultrathin sections from the middle of the explants were contrasted with 1% uranyl acetate and lead citrate. Grids were examined with a Philips Tecnai 12 electron microscope operated at 80 kV. Negative films were developed and scanned. For quantification of axonal degeneration at the EM level, we include in the category of degenerated axons those with collapsed myelin sheaths or axoplasm with undefined cytoskeletal structures and those that are less electron dense than control ones.
3D reconstruction
Tissue processing.
For three-dimensional reconstruction at the EM level, nerves were fixed in 3% glutaraldehyde in 0.05 m cacodylate buffer with 0.05% picric acid for 24 h at 4°C. After washing in cacodylate buffer, nerves were treated with 1% osmium tetroxide with 1.5% potassium ferrocyanide for 1 h. After washes in distilled water, nerves were immersed in 2% aqueous uranyl acetate for 1 h. Nerves were dehydrated and embedded with a Eponate 12 embedding kit (catalog #18010; Ted Pella).
Ultramicrotomy and EM.
Serial sections ∼100 nm were obtained and mounted in carbon Formvar-coated slot grids. Thin sections were contrasted using uranyl acetate and lead citrate. Observations of the grids were made using a Phillips Tecnai 12 Biotwin electron microscope; sequential photographs were taken and processed as described below.
Image processing.
Single photographs of the series were merged, and the stack was registered using the newstack and midas tools of the IMOD 3D rendering software (Kremer et al., 1996). The aligned stack was opened with the TrakEM2 plugin (Saalfeld et al., 2010, 2012) using the software Fiji (Schindelin et al., 2012). For the 3D reconstruction, manual tracing of the structures of interest was performed and rendered in 3D representation after edge smoothing.
Small interfering RNA reagents and transfection
We used the pooled SMART-selecte small interfering RNAs (siRNAs; Dharmacon) for targeting RyaR1 (L-082079-01-0005), RyaR3 (L-092028-01-0005), and IP3R1 mRNA (L-095360-01-0005). For control transfections, the control nontargeting siRNA (Dharmacon), which was designed with at least four mismatches to any known human or mouse gene, was used. Cells were transfected with 100 or 200 nm siRNAs using DharmaFect reagent (catalog #T-2003-02; Thermo Fisher Scientific) following the instructions of the manufacturer.
Quantitative RT-PCR analyses
Dissociated DRG neurons transfected with siRNA pools were analyzed at 3 d after transfection. RNA was extracted by the RNAeasy kit (catalog #74104; Qiagen), quantified, and reverse transcribed using Promega GoTag. Reverse-transcribed RNA samples were then amplified by quantitative PCR (qPCR) using the SybrGreen kit (catalog #; QT 605-05; Bioline Reagents). Primers used included the following: IP3R1, forward, GAGATGAGCCTGGCTGAGGTTCAA; reverse, TGTTGCCTCCTTCCAGAAGTGCGA; RyaR1, forward, TCTTCCCTGCTGGAGACTGT; reverse, TGGGAGAAGGCACTTGAGG; and RyaR3, forward, TCTTCCCTGCTGGAGACTGT; reverse, GTCTCCATGTCTTCCCGTA. Conditions for establishing the dissociation curves of the fluorophore with the amplified products were 10 min at 95°C, 5 min at 25°C, and then cycling for 1 min at 70°C and 1 min at 95°C. The relative levels of mRNA were normalized to rat GAPDH mRNA as an internal control (ΔCT), and RNA levels were then expressed relative to nontargeted siRNA control treated cells (ΔΔCT).
Tetramethylrhodamine methyl ester measurements of mitochondrial membrane potential
Tetramethylrhodamine methyl ester (TMRM; catalog #T-668; Invitrogen) is a cell-permeant, fluorescent dye that is readily sequestered by active mitochondria and then released from mitochondria during depolarization (Blattner et al., 2001). TMRM was added to a final concentration of 1 μm in HBSS and incubated for 30 min at 37°C protected from light. TMRM fluorescence was determined at 0 and 6 h post axotomy (hpa) using a spinning disk microscope (544 nm excitation and 590 nm emission filters). Change in fluorescence levels after different treatments were normalized to non-axotomized controls.
Intracellular calcium measurements in DRG neurons
DRG cell cultures were loaded for 30 min at 37°C in the dark with the cell-permeant AM of the fluorescent Ca2+ indicator fura-2 (5 μm). Simultaneously, cells were exposed to different drugs (Rya and U73122). To remove excess extracellular fura-2, glass coverslips were washed three times with HBSS. Intracellular fura-2 was alternately excited at 340 and 380 nm, and the emitted light was measured at a wavelength of 510 nm. The fluorescence ratio (340/380) provides a relative measure of the cytosolic-free Ca2+ concentration ([Ca2+]i).
Reactive oxidative species measurements
Immediately after axotomy, DRGs were incubated with standard culture medium plus one of the following drugs: 10 μm Trolox, 20 μm CsA, 2 μm U73122, 5 μm Rya, or 5 μm Ru360. Superoxide levels were measured using dihydroethidium (DHE) fluorescence 6 h after DRG axotomy in an epifluorescence microscope. Neurites were incubated in the last 30 min with 5 μm DHE under culture conditions. DHE fluorescence was obtained under a Cy5 emission filter. Superoxide levels on neurites were determined using NIH ImageJ colocalization highlighter plugin. Oxidative stress levels were calculated as the ratio of DHE fluorescent signal area in neurites and neurite length in every condition. Briefly, DHE signal and phase-contrast colocalization were used to obtain DHE area and neurite length. Photoshop (Adobe Systems) and NIH ImageJ were used to perform this analysis.
Analysis of ATP levels
Intraneuritic levels of ATP were assessed using magnesium green (MgGreen; catalog #M-3735; Invitrogen) indicator (Persson et al., 2013). In 7 DIV DRGs, MgGreen was added to a final concentration of 5 μm in HBSS and incubated for 30 min at 37°C protected from light. MgGreen fluorescence was determined at 0 and 6 hpa using a spinning disk microscope (488 nm). Change in fluorescence levels after different treatments were normalized to non-axotomized controls.
Results
Intracellular and extracellular calcium participates in the axonal degeneration cascade
To study the role of calcium in axonal degeneration, we first used an ex vivo model of injury-induced axonal degeneration of sciatic nerve explants, without contribution of the neuronal soma or the infiltrating immune cells, in which pharmacological treatments are well achieved and axonal degeneration can be readily evaluated (Tsao et al., 1999; Barrientos et al., 2011). In this model, we determined previously by Western blot and immunofluorescence that, at 3 d after injury, most axons in the nerve explant have degenerated (Barrientos et al., 2011).
Nerve explants were incubated for 3 d with the intracellular or extracellular calcium chelators BAPTA-AM or EGTA, respectively, and the degree of axonal degeneration was analyzed by immunofluorescence against neurofilament (NFH) that we characterized previously as a reliable readout for axonal degeneration in these explants (Barrientos et al., 2011). Compared with vehicle-treated sciatic nerve explants, both calcium chelators strongly protected from injury-induced axonal degeneration as shown by immunofluorescent images (Fig. 1A). Quantification of the axon density across multiple experiments (see Materials and Methods) showed significant protection from calcium chelation (Fig. 1B). These results confirm previous studies demonstrating that axonal degeneration is a calcium-dependent process (Schlaepfer and Bunge, 1973; George et al., 1995).
Differential effects of intracellular and extracellular calcium chelators on axonal mitochondria. Sciatic nerve explants from wild-type mice were incubated in vehicle solution (Veh), BAPTA-AM (100 μm), or EGTA (6 μm) for 3 d and analyzed by EM and immunofluorescence. A, Transverse sections of nerve explants stained for NFH. The NFH signal decreases considerably after 3 d in vehicle solution. In nerves treated with BAPTA or EGTA, strong preservation of NFH is observed. Scale bar, 20 μm. B, Quantification of axons positive for NFH in explant cross-sections as shown in A, expressed as axons per 100 μm2. A statistically significant difference in axonal density is seen after either of the calcium chelators treatment for 3 d (n = 3 per group; *p < 0.05 by Student's t test compared with 3 d of vehicle; error bars indicate SEM). C, At the ultrastructural levels, non-injured nerves show conserved axons and compacted myelin sheaths. Conversely, nerves incubated for 3 d in vehicle solution have degenerated axons and collapsed myelin sheaths. Only chelation of intracellular calcium using BAPTA-AM prevents mitochondrial swelling (inset). D, Quantification of mitochondrial diameters from transverse sections analyzed by EM in vehicle-, BAPTA-AM-, and EGTA-treated nerve explants (n = 3 per group; *p < 0.05 by Student's t test compared with 3 d of vehicle; error bars indicate SEM). Scale bars: C, 20 μm; inset, 300 nm. E, Embryonic DRG explants in vitro were axotomized and treated with Rya (5 μm) or U73122 (2 μm). At 6 hpa, cultures were treated for 30 min with fura-2. Treatment with either Rya or U73122 protects axons from intra-axonal calcium increase. The arbitrary color scale at the right indicates relative levels of calcium, with red and blue representing the high and low ends of calcium levels, respectively. Scale bar, 50 μm. F, Quantification of calcium increase by fluorescence intensity of ratio 340/380 (for details, see Materials and Methods). Either Rya (5 μm) or U73122 (2 μm) decreases calcium signal at 6 hpa (n = 9, 3 images per sample, 3 samples per condition, 3 independent experiments; *p < 0.05 by Student's t test compared with control; error bars indicate SEM).
Axonal degeneration requires mPTP opening (Barrientos et al., 2011). Because calcium is one of the main mPTP activators, this ion might be acting early after nerve injury in the cascade leading to axonal degeneration by activating the mPTP. Swelling of the axonal mitochondria attributable to the increase of mitochondria permeability represents a reliable ultrastructural indicator of mPTP opening (Hunter et al., 1976). This swelling takes place early during axonal degeneration (Vial, 1958; Webster, 1962; Barrientos et al., 2011). To test the effect of calcium chelation on mPTP opening, we measured mitochondrial diameter in sciatic nerve explants treated for 3 d with BAPTA-AM or EGTA. BAPTA-AM and EGTA protected axons from degeneration judged by both neurofilament immunofluorescence (Fig. 1A,B) and ultrastructural analyses (Fig. 1C). Surprisingly, mitochondrial diameter was differentially affected by intracellular or extracellular calcium chelation. Chelation of intercellular calcium by BAPTA-AM significantly inhibited swelling, but extracellular calcium chelation by EGTA had no significant effect on mitochondrial swelling compared with vehicle-treated explants (Fig. 1C,D).
These results show that chelation of external calcium can protect from neurofilament degradation and axonal disintegration and suggest that mPTP is activated by calcium released from internal stores.
Early calcium increase after axotomy is dependent on ER calcium channels
Axonal degeneration is a calcium-mediated event (Schlaepfer, 1974). It is well known that calcium activates proteases during the late stages of Wallerian degeneration (Schlaepfer and Hasler, 1979), and an early increase in intra-axonal calcium levels was described recently in primary cultures of axotomized sensory axons (Adalbert et al., 2012).
To study the role of intracellular calcium stores in the degeneration of axons, we first analyzed the effects of pharmacological inhibition of ER calcium channels on intra-axonal calcium levels after axotomy. We used cultures of sensory neurons from dorsal root ganglia (DRGs) devoid of Schwann cells (Lopez-Verrilli et al., 2013) and the fluorescent calcium indicator fura-2 for these studies. Compared with sciatic nerve explant, DRG neurons in vitro show very different timing of degeneration (Barrientos et al., 2011). We studies the time course of degeneration for DRG neurites after axotomy, and by 12 h, most axotomized DRG neurites were degenerated (data not shown). To test the contribution of intracellular calcium stores during Wallerian degeneration, we blocked RyaR or IP3R, which both function as ER calcium channels, using Rya or the phospholipase C inhibitor U73122, respectively. In these DRG neurons, axotomy results in a potent calcium increase in neurites at 6 hpa when compared with intact DRGs (Fig. 1E,F). Incubating DRGs with Rya or U73122 primarily prevented this calcium increase in injured DRGs (Fig. 1E,F). Together, these results indicated that calcium release from both RyaR and IP3R contributes to the early calcium increase after axotomy.
ER calcium channel blockers inhibit injury-induced axonal degeneration
In the cytoplasm, most calcium is sequestered in organelles, such as the ER and mitochondria, or bound to calcium-binding proteins. To test the contribution of ER calcium during Wallerian degeneration, we blocked the ER calcium channels using Rya or U73122.
Incubating nerve explants with Rya or U73122 significantly protects from axonal degeneration (Fig. 2A,B). To determine whether the protective effect of these drugs targets the axonal ER, we used DRG neurons devoid of Schwann cells (see Materials and Methods). After mechanical transection of DRG axons, degeneration of anucleated axons in vehicle-treated cultures was complete by 12 hpa based on NFH immunostaining (Fig. 2C). However, anucleated axons treated with Rya or U73122 appeared intact at 12 h (Fig. 2C). Quantifying the axonal degeneration index (see Materials and Methods) showed a significant decrease in degeneration for the Rya- and U73122-treated cultures (Fig. 2D). This protection seen with inhibition of RyaR and IP3R was comparable with that seen Ru360 that inhibits mitochondrial calcium uptake (Fig. 2B,D). Together, these results suggest that calcium release from either RyaR or IP3R in the axonal ER participates in axonal degeneration.
Pharmacological inhibition of ER calcium channels protects axotomy-induced axonal degeneration. Sciatic nerve explants from mice were incubated in vehicle (Veh) solution, Rya (50 μm), U73122 (20 μm), or Ru360 (50 μm) for 3 d and analyzed by immunofluorescence. A, Transverse sections of nerve explants stained for NFH. Axons in nerve explants incubated for 3 d with Rya, U73122, or Ru360 do not show signs of degeneration. Scale bar, 20 μm. B, Quantification of axons positive for NFH in explant cross-sections as shown in A, expressed as axons per 100 μm2. Statistically significant protection is seen after Rya, U73122, or Ru360 treatment at 3 d (*p < 0.05 by Student's t test compared with 3 d of vehicle; error bars indicate SEM). C, Embryonic DRG explants in vitro were axotomized and treated with Rya (5 μm), U73122 (2 μm), or Ru360 (5 μm). At 12 hpa, cultures were immunostained for NFH. Treatment with Rya, U73122, or Ru360 protects axons from axotomy-induced degeneration. Scale bar, 50 μm. D, Quantification of axonal preservation by the degeneration index (area neurites fragmented/total area; for details, see Materials and Methods). Rya, U73122, or Ru360 protects injury-induced neurite degeneration at 12 hpa (n = 9, 3 images per sample, 3 samples per condition, 3 repetitions; *p < 0.05 by Student's t test compared with control; error bars indicate SEM).
Genetic knockdown of ER calcium channels protects against axonal degeneration
To genetically test the contribution of ER calcium channels in axonal degeneration, we specifically depleted neurons of RyaR and IP3R using siRNA. In mammals, there are three RyaR isoforms and five IP3R isoforms (Ross et al., 1992). RyaR1, RyaR3, and IP3R1 are expressed in rat DRGs (Dent et al., 1996; Cheng et al., 2010).
To obtain higher transfection efficiency, we use dissociated DRG cultures cultured in transwell chambers, in which DRG neurons are seeded in the top compartment and extend neurites across a porous membrane to the lower compartment (Zheng et al., 2001). This approach was used previously to quantify axonal degeneration in DRG cultures (Schoenmann et al., 2010; Barrientos et al., 2011). Axotomy is performed by scraping away cell bodies, leaving isolated axons associated with the chamber membrane in the lower compartment. DRG neurons were transfected and neurite integrity was analyzed by immunostaining 12 hpa. RT-PCR showed that each siRNA pool specifically and significantly reduced mRNA levels of its target 3 d after transfection (Fig. 3E,F). The knockdown of either RyaR1/3 or IP3R1 strongly protected from the axonal degeneration induced by axotomy (Fig. 3A,B).
Genetic knockdown of RyaR and IP3R1 protects degeneration of mechanically or toxic injured axons. An siRNA pool was used to determine whether depletion of RyaR1 and RyaR3 (RyR1/3) or IP3R1 from dissociated DRG cultures protect from axotomy and vincristine-triggered axonal degeneration. DRGs were transfected with the siRNA pool or a control (Ctrl) nontargeting siRNA. A, Transfected DRG neurons were cultured on transwell chambers, allowing the separation of axonal processes from cell bodies. After 72 h of culture, neuronal cell bodies were scraped away from the upper membrane surface. Control siRNA-transfected neurons showed almost complete degeneration of distal axon 12 h after transection, but RyaR1/3 and IP3R1 siRNA transfected neurons showed preservation of axons over the same period of time. Scale bar, 50 μm. C, DRGs were transfected as in A and treated with vincristine (1 μm) to induce axonal degeneration. Both RyaR1/3 and IP3R1 siRNA-transfected neurons showed preservation of axons 12 h after vincristine treatment. B, D, Quantification of injury-induced degeneration of A and C using the degeneration index (n = 9, 3 images per sample, 3 samples per condition, 3 repetitions; *p < 0.05 by Student's t test compared with nontarget siRNA; error bars indicate SEM). E, Reduction of RyaR1, RyaR3, and IP3R1 mRNA after siRNA was assessed by qPCR in embryonic DRG neurons. E, F, Gel bands of each qPCR result. A significant decrease of RyaR1, RyaR3, and IP3R1 mRNA was observed after siRNA (n = 3 per each group; *p < 0.05 by Student's t test compared with nontarget siRNA; error bars indicate SEM).
To determine whether RyaR1/3 and IP3R1 play a role in axon degeneration from other stimuli, we used the chemotherapeutic agent vinblastine that disrupts microtubule-based axonal transport and triggers axonal degeneration in intact neurons (Ikegami and Koike, 2003). After depletion of either RyaR1/3 or IP3R1, axons were significantly protected from this chemical-induced axonal degeneration paradigm based on NF immunostaining (Fig. 3C,D).
Together, the above data indicate that chemical-induced axon degeneration and axotomy-induced Wallerian degeneration use mechanisms that require activation of both RyaR and IP3R.
Axonal ER is closely apposed to mitochondria and axoplasmic membrane
The ER is a multifunctional organelle responsible for protein synthesis and folding, vesicular trafficking, lipid biosynthesis, and calcium storage (Voeltz et al., 2002). In peripheral axons, the ER forms a continuous network of irregular tubules and cisternae (Tsukita and Ishikawa, 1976). To elucidate the participation of the ER in axonal degeneration, we undertook a detailed characterization of its structure and potential association with other intra-axonal organelles using a 3D reconstruction of the axonal ER, mitochondria, and axolemma by serial sectioning and electron microscopy (EM). Longitudinal serial sections of sciatic nerve myelinated axons showed that the ER forms a continuous network of smooth tubules of a constant diameter (Fig. 4A–C). Regularly, specializations of this structure are observed in the form of accumulation of vesicles (Fig. 4D). In addition, part of this ER network appears in close proximity to the axonal plasma membrane (Fig. 4A,B). When mitochondria were reconstructed along with the ER, regions of close proximity, <0.1 μm, between these two organelles were found (Fig. 4E,F).
3D axon reconstruction shows close appositions between mitochondria and ER organelles in peripheral axons. Sciatic nerves from wild-type mice were fixed, and serial sections at ∼80 nm intervals were obtained (see Materials and Methods). After section alignment, axonal membrane structures and mitochondria were reconstructed from myelinated fibers and rendered. Reticular structures are colored yellow, mitochondria in red, and the myelin sheath in blue. Selected micrographs from contiguous sections are included for each rendering. A, 3D reconstruction shows an extensive smooth endoplasmic reticulum (SER) network and elongated mitochondria of varying sizes. The right shows a higher magnification of the boxed area from the left. C, Quantification of axonal SER diameter. Different types of morphological configurations of the SER network are observed, including fibrillar (B) and specializations in the form of raceme-like structures (D). E, F, Regions of close proximity between mitochondria (M) and SER in the axonal compartment (see arrowheads in individual sections). G, Higher magnification of regions indicated by arrowheads in F. H, Cross-section of a myelinated axon in which a SER structure is closely apposed to two mitochondria. Scale bars, A, 1 μm; B, 500 nm; D, 250 nm; E, F, 200 nm; H, 200 nm.
These morphological data demonstrate that a continuous ER network is closely apposed with the axonal plasma membrane and mitochondria, an association that might provide a means for efficient communication between these organelles and also could be important during degenerative processes.
Inhibition of ER calcium channels prevents mPTP opening
We showed previously that axonal degeneration is mediated by opening of the mPTP (Barrientos et al., 2011). Mitochondrial calcium overload and oxidative stress are known to be major activators of the mPTP (Halestrap et al., 1997; Bernardi et al., 2006). Increase in mitochondrial calcium was detected 9 hpa and preceding the cytosolic increase (LoPachin and Lehning, 1997). The source of this early increase in mitochondrial calcium remains unknown, but the increase in axoplasmic calcium that we see from ER release appears to precede this change in mitochondrial swelling (Fig. 1E). Also, depletion of intracellular calcium stores protects against axonal degeneration under ischemic conditions (Stys, 2005), and ER stress induces calcium-dependent permeability transition, mitochondrial outer membrane permeabilization, and apoptosis (Deniaud et al., 2008). Thus, the axonal ER network, which is in close proximity to mitochondria in axons (Fig. 5), is a likely source of mitochondrial swelling.
Pharmacological inhibition of ER calcium channels protects against mitochondrial swelling. A, D, Representative electron micrographs of non-damaged neurites (0 h) and explants incubated for 1 or 3 d in vehicle (Veh) or ER calcium channels blockers Rya and U73122 or Ru360. Inhibition of ER channels or the mitochondrial uniporter prevents mitochondrial swelling at 24 hpa (A) and 72 hpa (D). Scale bars, 20 μm; inset, 300 nm. B, E, Quantification of mitochondrial diameters from transverse sections analyzed by EM in vehicle-, Rya-, U73122-, and Ru360-treated nerve explants. Mitochondrial swelling was inhibited by Rya, U73122, and Ru360 at 1 and 3 d (n ≥ 45 mitochondria/nerve over 3 separate experiments; *p < 0.05 by Student's t test compared with vehicle). C, F, Quantification of degenerated axons from transverse sections analyzed by EM in vehicle-, Rya-, U73122-, and Ru360-treated nerve explants. At 3 d, Rya, U73122, and Ru360 inhibit axonal degeneration of nerve explants (n = 3 per group; *p < 0.05 by Student's t test compared with 1 d (C) and 3 d (F) of vehicle; error bars indicate SEM). G, H, Embryonic DRGs were axotomized and treated with vehicle, Rya (5 μm), or U73122 (2 μm). After 6 h, they were stained with TMRM, a mitochondrial membrane potential vital dye. G, TMRM fluorescence in non-injured DRG neurites or 6 hpa DRGs treated with vehicle solution, Rya, U73122, or CsA. Rya, U73122, and CsA inhibit the loss of TMRM fluorescence after axotomy. Scale bar, 50 μm. H, Quantification of TMRM fluorescence in each condition normalized to control (n = 9, 3 images per sample, 3 samples per condition, 3 repetitions; *p < 0.05 by Student's t test compared with vehicle; error bars indicate SEM).
To determine whether ER-derived calcium activates the mPTP after axonal injury, we used EM to test for mitochondrial swelling in sciatic nerve explants incubated with ER channel blockers or vehicle for 24 and 72 h. This mitochondrial swelling is an ultrastructural consequence of mPTP opening (Vial, 1958; Webster, 1962). At 24 h, a small amount of vehicle-treated axons show signs of degeneration, and nerve explants incubated for 72 h in vehicle solution consist mainly of degenerated axons with collapsed myelin sheaths (Fig. 5A), consistent with our immunofluorescence data (Fig. 2A). As expected, in vehicle-incubated nerve explants, mitochondrial diameter increase was observed at 24 h in vitro with progressive enlargement up to 72 h (Fig. 5A,B,E; Barrientos et al. 2011). The nerve explants incubated with Rya or U73122 for 24 and 72 h showed axonal protection at the EM level (Fig. 5A,D). At 24 h, Rya- or U73122-treated nerve explants showed mitochondrial diameters similar to contralateral (0 h) nerves (Fig. 5A,B). Reduction of mitochondrial swelling was also observed in explants treated for 72 h with these drugs (Fig. 5D,E). Inhibition of the uniporter-mediated calcium entry into mitochondria by Ru360 also inhibited mitochondrial swelling and axonal degeneration at the EM level and by immunofluorescence analysis (data not shown).
mPTP opening leads to loss of the mitochondrial membrane potential (Halestrap, 2009). CsA prevents mPTP opening by binding to the mPTP regulatory protein Cyclophilin-D (Barrientos et al., 2011). Thus, we asked whether RyaR and IP3R inhibition might prevent loss of mitochondrial membrane potential in anucleated axons using the mitochondrial membrane potential indicator TMRM. Axotomy results in a profound loss of mitochondrial membrane potential, with a 50% reduction of TMRM fluorescence at 6 hpa when compared with intact DRGs (Fig. 5G). Conversely, in anucleated axons that were treated with Rya or U73122, the decrease in TMRM fluorescence was partially inhibited (Fig. 5H). Importantly, after CsA treatment, the injury-induced decrease in TMRM fluorescence was also inhibited (Fig. 5G,H).
Together, these EM and TMRM imaging data strongly suggest that activation of both RyaR and IP3R after axotomy participates in mPTP opening that leads to axotomy-induced Wallerian degeneration.
Calcium release from the ER leads to axonal degeneration by activating the mPTP
To determine whether there is a functional association between the axonal ER and mPTP in axonal degeneration, we used caffeine to directly activate RyaR in DRG cultures, and axonal degeneration was evaluated in conditions in which mPTP opening was pharmacologically inhibited. Caffeine treatment of intact sensory neuron cultures for 48 h led to extended beading along axons, suggesting damage to these processes (Fig. 6A). Importantly, beading was prevented by inhibition of RyaR (Fig. 6A). This suggests that the axonal beading injury that caffeine treatment elicited is through activation of the RyaR. This beading process appears to be a part of the injury-induced degeneration program, because axotomized neurites also exhibit beaded axons between 6 and 8 hpa (Fig. 6C,D).
Activation of RyaR triggers axonal degeneration, which is rescued by Rya or mPTP inhibition. A, Embryonic DRG cultures treated with caffeine (Caf, 10 mm), an activator of RyaR, caffeine plus Rya (5 μm), or caffeine plus CsA (20 μm) for 48 h. Caffeine alone induces the appearance of axonal beadings, a degeneration-like phenotype. Either Rya or CsA rescues this beaded phenotype. B, Quantification of the beading density in cultures treated as in A (n = 9, 3 images per sample, 3 samples per condition, 3 repetitions; *p < 0.05 by Student's t test compared with 48 h of caffeine; error bars indicate SEM). Scale bar, 50 μm. C, Time course of beading in vitro. Embryonic DRGs were axotomized and immunostained for NFH at 3, 6, 8, 10, and 12 h. At 6 hpa, a degenerated phenotype that shows beads in the neurites start to appear. At 8 hpa, the beading phenotype is clear. Scale bar, 50 μm. D, Quantification of beading after axotomy at different times, with a significant increase in beading at 8 hpa observed when compared with non-injured neurites (n = 3 per each group; *p < 0.05 by Student's t test compared with control; error bars indicate SEM). E, F, Embryonic DRGs were axotomized and treated with vehicle (Veh), Rya (5 μm), or U73122 (2 μm). After 6 h, they were stained with MgGreen, an ATP-sensitive dye. E, MgGreen fluorescence in non-injured DRG neurites or 6 hpa DRGs treated with vehicle solution, Rya, or U73122. After axotomy, fluorescence increases, denoting a drop in ATP levels. Both Rya and U73122 inhibit the axotomy-induced fluorescence increase. Scale bar, 50 μm. H, Quantification of MgGreen fluorescence in each condition shown as the reciprocal of the signal in each condition normalized to control (relative ATP−1; n = 9, 3 images per sample, 3 samples per condition, 3 repetitions; *p < 0.05 by Student's t test compared with vehicle; error bars indicate SEM).
To test for participation of the mPTP in caffeine-induced axonal damage, we treated DRG cultures with a combination of caffeine to activate RyaR and CsA to inhibit mPTP opening. Interestingly, the mPTP inhibition with CsA significantly decreased axonal beading in caffeine-treated neurons (Fig. 6A,B). We next assessed the impact of axonal damage in mitochondrial function by measuring ATP levels. We used the MgGreen assay, which indirectly measures the change in cellular ATP concentration, with ATP reduction seen as an increase in fluorescence intensity (Baggaley et al., 2008; Persson et al., 2013). Axotomy led to increased signal intensity of MgGreen in neurites, indicating reduced levels of cellular ATP compared with non-injured neurites (Fig. 6E,F). After both Rya and U73122 treatments, the reduction of ATP levels triggered by axotomy were partially inhibited at 6 hpa (Fig. 6E,F).
These results demonstrate that ER-derived calcium directly contributes to mitochondrial dysfunction in the axonal compartment and suggest that ER-derived calcium triggers mPTP opening in mitochondria during the early stages of axonal injury. This event likely leads to a second phase of injury/degeneration that is associated with mitochondrial dysfunction and protease activation.
Inhibition of ER calcium release protects against axotomy-induced oxidative stress
Both calcium and reactive oxidative species (ROS) can activate the mPTP (Halestrap, 2009). This can lead to successive release of calcium and ROS production (Court and Coleman, 2012). Increasing the neuronal antioxidant capacity was shown to protect from injury-induced axonal degeneration (Calixto et al., 2012).
Because we demonstrated a functional relationship between ER-derived calcium release and mPTP opening (Figs. 5, 6), we asked whether ROS production might depend on this ER-dependent process. To this end, we fluorometrically measured superoxide levels in DRG neurites in vitro after axotomy and in DRGs treated with ER and mitochondrial calcium channels inhibitors. After axotomy, a strong increase in superoxide levels was detected at 6 h (Fig. 7A,C). This precedes the decay in neurofilament immunostaining that was seen 12 hpa (Fig. 2C), so it likely represents an early event in axonal degeneration. Treatment with the antioxidant Trolox or mPTP inhibition using CsA abolished the increase in superoxide levels triggered by axotomy (Fig. 7B). This demonstrates that oxidative stress is downstream of mPTP opening during axonal degeneration.
ER channel blockers inhibit the increase in oxidative stress triggered by axotomy. Superoxide levels in neurites were measured using DHE (5 μm). Embryonic DRGs were cultured for 7 d, axotomized, and immediately treated with Rya (5 μm), U73122 (2 μm), Ru360 (5 μm), Trolox (10 μm), or CsA (20 μm). After 6 h, superoxide levels were assayed by culturing neurites with DHE and observed by phase-contrast bright-field (BF; left) and fluorescence microscopy (right). A, DHE fluorescence is almost not detectable in non-injured neurites (0 h). At 6 hpa, the levels of DHE fluorescence strongly increase in axotomized neurites [vehicle (Veh)]. This increase in DHE fluorescence is inhibited by treatment with the ER channel inhibitors Rya and U73122, the mitochondrial uniporter inhibitor Ru360, the antioxidant Trolox, and the mPTP inhibitor CsA. Notice that, in all cases, neurites appear relatively conserved at 6 hpa by bright field. Scale bar, 50 μm. B, The DHE signal in neurites was calculated as the ratio of the total area of DHE signal in neurites normalized by the total neurite length in the corresponding field (n = 3 samples, 2 images per sample; *p < 0.05 by Student's t test compared with vehicle; error bars indicate SEM).
In this context, the axotomy-induced calcium release from the ER could be modulating the observed increase in oxidative stress. To test this possibility, we evaluated superoxide levels in anucleated axons in which the RyaR or IP3R were inhibited. Anucleated neurites treated with Rya or U73122 showed superoxide levels comparable with nondamaged neurites (Fig. 7A–C). Finally, neurites treated with the mitochondrial calcium uniporter using Ru360 also showed no rise in superoxide levels after axotomy (Fig. 7A–C). These results indicate that the increase in oxidative stress after axotomy depends on calcium release from the ER, most likely downstream mPTP opening after mitochondrial calcium uptake.
Discussion
Axonal degeneration is an early feature of several neurodegenerative conditions, but the sequence of events leading to this axonal loss is still unknown. The role of calcium in this degenerative process has long been accepted, but there is no detailed understanding of the calcium source(s) and dynamics. Chelation of extracellular calcium protects against axonal degeneration triggered by axotomy (George et al., 1995), and depletion of intracellular calcium stores protects against axonal degeneration under ischemic conditions (Stys, 2005). We showed previously that a crucial event during axonal degeneration is the opening of the mPTP (Barrientos et al., 2011), which is highly dependent on calcium levels (Halestrap et al., 1986). The data that we present here establishes that the release of ER-derived calcium store is a critical step in Wallerian degeneration by activating the mPTP that leads to axonal loss.
Our results show that chelation of external calcium can protect from neurofilament degradation and axonal disintegration but not mitochondrial swelling (Fig. 1A,B). Conversely, calcium from internal stores probably regulates a step upstream of mPTP opening, suggesting that there are two calcium-dependent steps during axonal degeneration: one in which mPTP is activated primarily by calcium from internal stores and a later step downstream of mitochondrial dysfunction in which proteases are activated primarily by extracellular calcium.
Local ER calcium was implicated previously in degeneration only in an ischemic injury paradigm (Stys, 2005), but the participation of mitochondria as an intermediate stage has not been evaluated. Consistent with the observations in ischemic axonal injury, degeneration of axons after both mechanical- and toxic-induced axonal injuries was attenuated by pharmacological inhibition and genetic knockdown of the ER calcium channels RyaR and IP3R (Fig. 2). The mechanism of axonal degeneration during ischemia probably involves a decrease in axonal ATP and dysregulation of ionic composition, leading to membrane depolarization and intra-axonal calcium overload (Stys, 2004). Membrane depolarization during pathological conditions was shown to activate voltage-sensitive calcium channels, and inhibition of L- or N-type channels partially protects against anoxic and traumatic axonal injury (Fern et al., 1993; Imaizumi et al., 1999; Wolf et al., 2001; Ouardouz et al., 2006).
Inhibition of either RyaR or IP3R attenuated axonal degeneration triggered by both mechanical and toxic insults (Fig. 2) to approximately equivalent degrees. Studies in smooth muscle and cardiomyocytes demonstrated that activation of IP3R can contribute to RyaR-mediated calcium signaling, shaping the spatial distribution and kinetic properties of calcium sparks in these cells (Gordienko and Bolton, 2002; Zhang et al., 2003; Mackenzie et al., 2004; Zima and Blatter, 2004; Li et al., 2005; Domeier et al., 2008). Also, it was described recently that RyaR-mediated calcium sparks in skeletal muscle fibers are suppressed by pharmacological or genetic inhibition of IP3R channels (Tjondrokoesoemo et al., 2013). Our data are consistent with a functional link between these channels and suggest that activation of both RyaR and IP3R are required to trigger the downstream degenerative cascade that results in axonal mPTP opening. Therefore, the inhibition of one ER channel in the axons could affect the release efficiency of the other axonal ER channel. It was suggested that the ER contains two distinct calcium storage compartments: one associated with calcium release through the RyaR and the other associated with calcium release through the IP3R (Bian et al., 1991; Berridge, 1993; Tanaka and Tashjian, 1993; Tribe et al., 1994). Therefore, the amount of calcium released by only one channel after pharmacological or genetic inhibition of the other channel type might not be sufficient to reach the threshold for mPTP opening. More work will be required to clearly establish the interplay between axonal RyaR and IP3R for axonal pathophysiology.
After axotomy or toxic damage, inhibition of mPTP opening prevents axon degeneration (Barrientos et al., 2011). Mitochondrial swelling, a well established reporter of mPTP opening, can be seen in axons within ∼12 h after injury (Barrientos et al., 2011). Here, we showed that blocking ER channels prevented this mitochondrial swelling up to 72 hpa (Fig. 5). Also, the RyaR agonist caffeine can trigger axonal degeneration by itself, but mPTP inhibition using CsA prevents caffeine-induced axonal degeneration (Fig. 6). This places the ER calcium release as a critical regulator of mitochondrial dysfunction by mPTP opening.
We and others showed previously that increase in oxidative stress is a key and early step in axonal degeneration (Press and Milbrandt, 2008; Fischer and Glass, 2010; Calixto et al., 2012; O'Donnell et al., 2013). Consistent with these observations, ROS levels increase as early as 6 hpa in isolated axons. It was shown that an increase in mitochondrial calcium levels induces ROS production in a physiological context (Peng and Jou, 2010), and mitochondria depolarization leads to an increase in ROS production (Murphy, 2009). Thus, mPTP opening induced by ER-derived calcium will lead to an increase in ROS production, creating a feedforward loop of mitochondrial calcium overload, mPTP opening, and ROS production (Court and Coleman, 2012). Accordingly, inhibition of mPTP with CsA delays the axotomy-induced increase in ROS levels in axons, which is consistent with a proposed role for the mPTP as a regulator of mitochondrial calcium and ROS (Peng and Jou, 2010).
Putting the sum of our data together with previous studies generates a model in which early calcium release from ER triggers mPTP opening and disruption of mitochondrial function. This disruption eventually increases ROS and leads to additional calcium release and entry, triggering protease activation and cytoskeletal degradation (Fig. 8). A deeper understanding of the interplay between the ER and mitochondrion crosstalk will clearly be necessary to determine how to break the feedforward mechanism that results in additional calcium release and eventual degeneration of the axon.
Model of ER participation in axonal degeneration. Schematic representation of a neuron with soma and its axon. A mechanical injury (red bolt) isolates the axon and disrupts calcium equilibrium in the axon. Activation of voltage (discontinuous line to RyR) and metabotropic channels (discontinuous line to IP3R) in the axonal plasma membrane can induce ER calcium release by RyaR and IP3R. Calcium release from the ER is buffered by associated mitochondria (MITO; continuous black line). Crosstalk between RyaR and IP3R (dotted line) might explain that inhibition of one ER channel might affect the release efficiency of the other one. Alternatively, the efficiency of calcium release can be compromised with only one functional channel. Mitochondrial calcium overload results in mPTP opening, which leads to calcium overload in the axon, increase in ROS generation (black line between mPTP and ROS; red arrow shows increase), and decrease in ATP production (black arrow from mPTP to ATP; red arrow shows decrease), which changes the direction of the Na2+/Ca2+ antiporter and additional calcium enters from the extracellular space (black arrow), which potentially induces cytoskeleton disintegration through calpain activation.
Although the functional role for the ER in the axonal compartment has not been studied extensively, early morphological data showed an extensive ER network in axons (Droz et al., 1975; Tsukita and Ishikawa, 1976). Our 3D EM reconstructions are consistent with this report but also show that the axonal ER is closely juxtaposed to both mitochondria and the axolemma. These ultrastructural data suggest that axonal mitochondria may be topographically situated to readily take up calcium released by ER channels after axonal damage. Specialized ER membrane subdomains that regulate ER-to-mitochondria communication, referred to as mitochondria-associated ER membranes (MAM), were defined in other cellular systems (Fujimoto and Hayashi, 2011). These structures trigger take up of calcium into the mitochondrial lumen directly from IP3R or RyaR at the ER (Kirichok et al., 2004; Fujimoto and Hayashi, 2011). It will be interesting to determine whether such MAMs are also present in the axonal domain. Mutations in proteins responsible for maintaining the ER structure were demonstrated in hereditary spastic paraplegias (Park et al., 2010), and mutations in mitofusin 2 leads to an axonal form of Charcot-Marie-Tooth disease (CMT); this protein was shown to tether the ER to mitochondria (de Brito and Scorrano, 2008). Axonal degeneration is seen in both the hereditary spastic hereditary paraplegias and axonal CMTs (Kijima et al., 2005; Chen and Chan, 2006), so the ER-to-mitochondrial signaling that we define here could contribute to axon degeneration in human neurodegeneration. Additional work will be needed to determine whether the ER-to-mitochondrial calcium signaling uncovered here is relevant for the axonal loss in these and other neurodegenerative disorders. Nonetheless, it is intriguing to speculate that the active participation of the ER in axonal degeneration through early calcium release leading to mitochondrial dysfunction may provide new therapeutic targets for neuroprotection.
Footnotes
This work was supported by National Fund of Scientific and Technological Development Grants 1070377 and 3130610 (R.V.), Millennium Nucleus Grant RC120003, Ring Initiative ACT1109 (F.A.C.), National Institutes of Health Grant R01-NS04156 and Dr. Miriam and Sheldon G. Adelson Medical Research Foundation (J.L.T.). We thank Monica Perez for excellent EM processing.
The authors declare no competing financial interests.
- Correspondence should be addressed to Felipe Court, Department of Physiology, Faculty of Biology, Pontifical Catholic University of Chile, Avenida B. O'Higgins 340, Santiago 8331150, Chile. fcourt{at}bio.puc.cl; court.felipe{at}gmail.com
