Abstract
In addition to innervating the cerebral cortex, basal forebrain cholinergic (BFc) neurons send a dense projection to the basolateral nucleus of the amygdala (BLA). In this study, we investigated the effect of near physiological acetylcholine release on BLA neurons using optogenetic tools and in vitro patch-clamp recordings. Adult transgenic mice expressing cre-recombinase under the choline acetyltransferase promoter were used to selectively transduce BFc neurons with channelrhodopsin-2 and a reporter through the injection of an adeno-associated virus. Light-induced stimulation of BFc axons produced different effects depending on the BLA cell type. In late-firing interneurons, BFc inputs elicited fast nicotinic EPSPs. In contrast, no response could be detected in fast-spiking interneurons. In principal BLA neurons, two different effects were elicited depending on their activity level. When principal BLA neurons were quiescent or made to fire at low rates by depolarizing current injection, light-induced activation of BFc axons elicited muscarinic IPSPs. In contrast, with stronger depolarizing currents, eliciting firing above ∼6–8 Hz, these muscarinic IPSPs lost their efficacy because stimulation of BFc inputs prolonged current-evoked afterdepolarizations. All the effects observed in principal neurons were dependent on muscarinic receptors type 1, engaging different intracellular mechanisms in a state-dependent manner. Overall, our results suggest that acetylcholine enhances the signal-to-noise ratio in principal BLA neurons. Moreover, the cholinergic engagement of afterdepolarizations may contribute to the formation of stimulus associations during fear-conditioning tasks where the timing of conditioned and unconditioned stimuli is not optimal for the induction of synaptic plasticity.
Introduction
Basal forebrain cholinergic (BFc) neurons contribute dense projections to the entire cortical mantle (Zaborszky et al., 2011), particularly limbic areas (Mesulam, 2004), and to a few subcortical structures, such as the BLA (Ben-Ari et al., 1977; Nitecka and Frotscher, 1989). In both the neocortex and BLA, a subpopulation of interneurons expresses muscarinic type-2 (M2) receptors (Levey et al., 1991; McDonald and Mascagni, 2011) whereas principal cells express M1 receptors (Mrzljak et al., 1993; McDonald and Mascagni, 2010; Yamasaki et al., 2010). Pharmacological activation of muscarinic receptors elicits similar responses in principal neurons of the neocortex and BLA. Generally, it was found that ACh reduces spike afterhyperpolarizations and generates a slow EPSP through the inhibition of the M current (Metherate et al., 1992; Washburn and Moises, 1992; Womble and Moises, 1992, 1993; Pape and Driesang, 1998; Faber and Sah, 2002; Krnjević, 2004). However, in some cases, it was reported that the slow EPSP was found to be at least partly dependent on the activation of ICAN (Yajeya et al., 1997, 1999).
Consistent with the high density of cholinergic inputs to BLA neurons, ACh strongly modulates performance on BLA-dependent tasks that probe the formation of emotional memories (Gold, 2003; Tinsley et al., 2004). For instance, local intra-BLA infusion of muscarinic antagonists during training interferes with subsequent memory performance in conditioned place-preference tasks (McIntyre et al., 1998). Consistent with this, the concentration of ACh increases markedly in the BLA during training on BLA-dependent tasks (McIntyre et al., 2003). Moreover, a strong correlation was found between individual variations in ACh release in the BLA during training and subsequent memory recall (McIntyre et al., 2003).
Until recently, the impact of cholinergic inputs on neocortical and BLA neurons was mainly studied using application of drugs in vitro. However, exogenous agonists possibly affect synaptic versus extrasynaptic receptors to different degrees than the physiological release of neurotransmitters, potentially eliciting effects that are not normally evoked by endogenous transmitter release. Thus, we aimed to study the impact of cholinergic inputs on BLA neurons under conditions that more closely reproduce normal ACh release and are therefore less likely to recruit extrasynaptic cholinergic receptors. To this end, we combined optogenetic tools (Zhang et al., 2007) and in vitro patch-clamp recordings from BLA neurons in acute slices. Adult transgenic mice expressing cre-recombinase under the promoter of ChAT were used to selectively transduce BFc neurons with channelrhodopsin-2 (ChR2) and a YFP through the injection of an AAV. Slices of the amygdala were prepared from AAV-injected mice and BFc inputs were selectively stimulated with blue light while recording BLA neurons.
Our results indicate that the impact of BFc inputs on principal BLA neurons depends on their level of activity: excitatory when they are strongly activated and inhibitory during quiescence. Overall, these results suggest that by reducing the excitability of less active neurons and enhancing that of the more active ones, BFc neurons increase signal-to-noise ratio in the BLA.
Materials and Methods
Procedures were approved by the Institutional Animal Care and Use Committee of Rutgers University, in compliance with the Guide for the Care and Use of Laboratory Animals (Department of Health and Human Services).
Animals and procedures for AAV infusions.
All experiments were performed in ChAT-IRES-cre transgenic mice (B6;129S6-ChATtm1(cre)Lowl/J; The Jackson Laboratory) of either sex. When they were 2.5–3 months old, mice were anesthetized with isoflurane and placed in a stereotaxic apparatus (n = 31). They then received multiple subcutaneous injections of the local anesthetic bupivacaine in the region of the scalp to be incised. Ten minutes later, the skull was exposed and small holes were drilled above the regions of interest. We then performed bilateral infusions (0.33 μl/side) of an AAV (AAV2/5.EF1a.DIO.hChR2(H134R)-EYFP.WPRE.hGH; Penn Vector Core) in the basal forebrain [coordinates with respect to bregma (in μm): AP: −700, LM: 1750, DV: 4260; ∼0.33 μl each]. To this end, a glass pipette (∼50 μm outer tip diameter) containing the concentrated virus stock solution was lowered to the appropriate depth using a Nanoject-2 (Drummond Scientific) pressure-injection apparatus. After the surgery, the animals were placed in a ventilated biosafety cabinet located in a quarantine room for ≥7 d. The mice were used for electrophysiological recordings within 1.5–2 months of the AAV infusion (4–5 months old). In addition, three mice ranging in age between 4 and 12 months were used for anatomical experiments (see below).
Preparation of amygdala slices and electrophysiology.
Mice were deeply anesthetized with ketamine and xylazine (150 and 30 mg/kg, i.p., respectively) and transcardially perfused with 10 ml of an ice-cold solution containing the following (in mm): 2.5 KCl, 2.3 MgCl2, 26 NaHCO3, 1.2 NaHPO4, 10 glucose, and 242 choline. The brains were then removed from the skull and 300-μm-thick sections containing the BLA were obtained with a vibrating microtome using the same solution. BLA slices were then transferred to a holding chamber filled with an oxygenated (with 95% O2, 5% CO2) ACSF solution containing the following (in mm): 124 NaCl, 2.5 KCl, 1.2 NaHPO4, 26 NaHCO3, 1.3 MgCl2, 2 CaCl2, and 10 glucose. The osmolarity of the ACSF was 300 ± 3 mOsm.
One hour or more later, one slice was transferred to a custom-made recording chamber superfused with oxygenated ACSF (3–5 ml/min) and kept at 32°C. BLA neurons were visualized with an Olympus BX51WI microscope, equipped with infrared differential contrast optics and epifluorescence. Under visual guidance, we obtained whole-cell recordings of BLA neurons using pipettes (3–6 mΩ) pulled from borosilicate glass capillaries and filled with a solution containing the following (in mm): 130 KMeSO4, 10 NaCl, 2 MgCl2, 10 HEPES, 3 Na2ATP, 0.3 GTP, and 1 EGTA. With the exception of paired recordings, we patched a single cell per slice to minimize the risk that fiber fatigue developed as a result of light stimuli.
For perforated patch-clamp recordings, amphotericin B was dissolved in DMSO in a 60 mg/ml stock solution. A final concentration of 0.24 mg/ml amphotericin was added into the intracellular solution. Stocks were prepared daily and final solutions were renewed approximately every 3 h. To achieve successful recordings, the tips of pipettes were filled with amphotericin B-free solution. Generally, it took 30–45 min for the recordings to stabilize at an access resistance between 25 and 35 MΩ. Access resistance was monitored at regular intervals (∼2 min) during the recordings and calculated from the linear portion of the voltage response to a −100 pA intracellular current injection. In addition, we obtained measurements of access resistance before and after each experimental manipulation with the membrane test function of the pClamp software. The access resistance of our perforated patch recordings (25–35 MΩ) was not much higher than that typically accepted for whole-cell recordings (<20 MΩ) in the literature. Moreover, the measurements we obtained [membrane potentials, afterdepolarizations (ADPs), and muscarinic IPSPs] are not very vulnerable to small perturbations in access resistance because they are slow events. Finally, we discarded all recordings that displayed abnormalities during current injections.
In all cases, Alexa 594 (50 μm) was added into the intracellular solution. During perforated patch recordings, this allowed us to both ascertain the integrity of the perforated patch and visualize the dendrites of recorded neurons. To this end, at the end of the recordings, gentle suction was applied to rupture the patch and allow Alexa 594 to diffuse into the cells. In contrast, in whole-cell mode, Alexa 594 diffused throughout the cells during the recordings.
Current- and voltage-clamp recordings were obtained with an Axoclamp 900A amplifier and digitized at 10 kHz with a Digidata 1440A interface (Molecular Devices). Principal and local-circuit neurons of the BLA were distinguished using a combination of well established morphological and electrophysiological criteria (McDonald, 1992; Sah et al., 2003; Spampanato et al., 2011). Principal cells had densely spiny dendrites and generated spikes trains that exhibited variable degrees of spike frequency accommodation. Given that BLA interneurons exhibit heterogeneous firing patterns (Woodruff and Sah, 2007; Jasnow et al., 2009; Sosulina et al., 2010; Spampanato et al., 2011; Mańko et al., 2012), we relied primarily on morphological criteria (paucity of dendritic spines) to identify these cells.
Optogenetic activation of BFc axons was achieved with whole-field application of blue-light stimuli (460 nm, 5 ms pulses, every 15–30 s). BFc stimuli were paired with intracellular current pulses of varying amplitudes, the order of which was randomized. For each current intensity, we interspersed trials with and without stimulation of BFc axons.
In some experiments, we tested the effects of carbachol (CCh) in principal BLA neurons. CCh (3 μm or 10 μm) was applied via pressure ejection from a patch pipette or by addition of the drug to the perfusate. For pressure applications, patch pipettes containing CCh (dissolved in ACSF) were positioned at proximity (∼50 μm) of the recorded neurons. Pressure (∼10 psi) was applied to the back of the pipette with a 10 cc syringe. In the CCh experiments, only one cell per slice was tested to avoid receptor desensitization.
Analyses and statistics.
Electrophysiological data were analyzed off-line using Clampfit 10 (Molecular Devices). The onset of muscarinic IPSPs was defined as the time after the end-of-light stimuli when the membrane potential became more negative than prestimulus values. Their amplitude was measured by averaging membrane potential values during a 10 ms segment centered on their peak. Their offset was defined as the time when membrane potential values returned to baseline. The onset of ADPs was defined as the time when, following offset of depolarizing current pulses, the membrane potential became more positive than baseline (prestimulus) values. Peak ADP amplitudes were measured by averaging the membrane potential values during a 100 ms segment centered on their peak. ADP offset was defined as the point where the membrane potential returned to prepulse values. For assessing the relation between current-evoked firing frequency and the amplitude of the ensuing ADPs, we used a polynomial fit with a minimum number of terms that result in residuals accounting for a p of 0.05 or less. During pharmacology experiments with carbachol, input resistance was regularly monitored with −100 pA current pulses. Means were compared with two-tailed Student's t tests using SPSS software. The threshold p value for significance was 0.05.
Biocytin visualization.
Following recordings with biocytin in the pipette (0.2%), slices were transferred to 4% paraformaldehyde for at least 24 h for fixation. For biocytin visualization, slices were incubated in 1:200 Cy3-conjugated streptavidin or Texas Red-conjugated streptavidin (Invitrogen; Jackson ImmunoResearch) for 20–24 h. Slices were then transferred to a slide and mounted with Vectashield wet mounting medium (Vector Laboratories). For the acquisition of digital images Adobe Photoshop was used with an AxioCam camera attached to a Zeiss microscope. In some cases, neuronal morphology was revealed using nickel-3,4 diaminobenzidine (Ni-DAB) as follows: the slices were washed first in 0.01 m PBS four times for 10 min each. Subsequently, the slices were left for overnight incubation in avidin-biotinylated horseradish peroxidase (ABC 1:100 on PB-0.3% Triton X-100) at room temperature. On the next day, the slices were washed in 0.1 m PBS twice for 10 min each. Then, the slices were incubated in Ni-DAB for 3–10 min (2 ml of 1% nickel ammonium sulfate in TBS, 2 ml of 1% ammonium chloride in TBS, TBS pH 7.60 up to 50ml, 1 ml of 20 mg/ml DAB Stock; 20 μl/ml of 0.03% hydrogen peroxide), air dried overnight, and coverslipped using DEPEX.
Immunohistochemistry for ChAT.
Animals were deeply anesthetized with urethane (>1.2 g/kg) and then transcardially perfused with saline followed by 4% paraformaldehyde in 0.1 m PB. The brain was removed and placed into the same solution for overnight fixation. The next day, the brains were placed into a sucrose solution (30%). Three days later, 50-μm-thick sections were cut using a freezing microtome. Sections were placed into 0.1 m PB for ∼24 h. Every third section was taken for immunohistochemistry. Sections were washed three times in 0.1 m PB and incubated with goat-anti-ChAT (1:500; Millipore) antibody with 1% normal donkey serum and 0.5% Triton-X in 0.1 m PB for overnight incubation at room temperature under dark. The next day, sections were washed three times in 0.1 m PB and transferred into donkey-anti-Goat Cy3 IgG (1:100) with 1% normal donkey serum and 0.5% Triton-X in 0.1 m PB for ∼3 h at room temperature under dark. Finally, sections were washed three times in 0.1 m PB and mounted using Vectashield wet mounting medium.
Identification of principal cells and local-circuit neurons.
Prior studies that correlated the physiological and morphological properties of BLA neurons have revealed that principal neurons come in two main varieties (for review, see Sah et al., 2003; Spampanato et al., 2011; Duvarci and Pare, 2014): regular spiking and, less frequently, intrinsically bursting. This is in contrast to most interneurons that show a fast-spiking behavior with little spike frequency adaptation and generate much briefer spikes than principal cells. However, other firing patterns have been observed in interneurons (Rainnie et al., 2006; Woodruff and Sah, 2007; Jasnow et al., 2009; Sosulina et al., 2010). Therefore, lack of fast-spiking pattern does not necessarily mean the recorded cell is not an interneuron. Consequently, we relied on morphological criteria to distinguish principal cells and interneurons (McDonald, 1992). Principal cells were identified as such by their high density of dendritic spines. Neurons that were aspiny or sparsely spiny were classified as interneurons.
Results
Correspondence between YFP-labeled and ChAT elements
Previously, it was reported that the BLA contains a very high concentration of ChAT-immunopositive axons (Carlsen and Heimer, 1986; Nitecka and Frotscher, 1989; Kitt et al., 1994) and that BFc neurons contribute this dense cholinergic innervation (Woolf et al., 1983; Carlsen et al., 1985; Nitecka and Frotscher, 1989). Therefore, we first verified whether YFP-expressing BFc neurons at the virus infusion site were also immunoreactive for ChAT. As shown in Figure 1A, a perfect correspondence was found between YFP and ChAT-expressing BFc neurons. That is, all YFP-expressing basal forebrain neurons were also ChAT immunoreactive.
Correspondence between YFP and ChAT expression. A, Basal forebrain (BF) neurons near the virus infusion site that express YFP (A1) and are immunopositive for ChAT (A2). A3, Overlay of A1 and A2. A1–A3, Show the same field of view. B, YFP-labeled (B1) and ChAT-immunoreactive (B2) axons in the BLA are similarly distributed. B3, Overlay of B1 and B2. B1–B3, Show same field of view. C, Micropipette filled with Alexa 594 and recently patched soma surrounded by YFP-labeled axons. Scale bars: A1–A3, 100 μm; B1–B3, 1 mm; C, 15 μm.
In addition, we examined whether the distribution of YFP-labeled axons in the amygdala matched that of ChAT-immunopositive axons. As shown in Figure 1B, a close correspondence was found between the two. Similar to the distribution of ChAT-immunopositive fibers (Fig. 1B2), a much higher concentration of YFP-labeled axons was seen in the BLA than the central and medial nuclei (Fig. 1B1), in keeping with prior descriptions (Carlsen and Heimer, 1986; Kitt et al., 1994).
Database
We obtained patch recordings of BLA neurons and examined the impact of BFc inputs, when activated by brief (5 ms) pulses of blue light. To minimize the possibility of false negatives, we only recorded neurons located in a dense plexus of YFP-labeled axons (Fig. 1C). Consistent with the fact that principal cells account for the majority of BLA neurons (McDonald, 1992), most of our recordings were obtained from this cell type (n = 67; 51 in whole-cell mode; 16 in perforated-patch mode). These recordings were obtained from 31 mice. Typically two to three whole-cell recordings or one to two perforated-patch recordings were obtained per animal. Only one neuron was recorded per slice.
In addition, a minority of recordings was obtained from aspiny neurons presumed to be local-circuit cells: fast-spiking (FS) neurons (n = 6; Fig. 2A1,A2) and late-firing (LF) cells (n = 5; Fig. 2B1,B2). We begin with a description of the results obtained in these rare cell types. The rest of the Results section will be devoted to principal cells (Figs. 3⇓⇓–6).
Contrasting physiological properties and BFc responsiveness of FS and LF neurons. Representative FS (A) and LF (B) neurons. Morphological properties of FS cell (A1) revealed with post hoc visualization of biocytin and LF neuron (B1) with Alexa 594. Region enclosed in rectangle in B1 is expanded on the right. A2, B2, Voltage responses to current pulses in FS and LF cells, respectively. FS cell can fire action potentials at high rates with little frequency accommodation. LF cell displays conspicuous delay to firing and spike-frequency accommodation. A3, B3, Paired recordings of FS (A3) or LF (B3) cell (current-clamp mode, top) with a principal neuron (voltage-clamp mode, bottom; holding potential −50 mV) reveal that both interneurons form GABAergic synapses with principal cells. Colored traces are the average of the black traces. B4, Left, Light-induced activation of BFc axons elicits nicotinic EPSPs in LF cell. Right, BFc-evoked EPSP is abolished by mecamylamine (10 μm).
Responses of principal BLA neurons to BFc inputs. A, Voltage response of principal BLA neuron (top) to current pulses of gradually increasing amplitude (bottom). B, Morphological properties of two principal BLA neurons as identified post hoc with biocytin (B1) or during the experiment with Alexa 594 (B2). Regions enclosed in dashed lines in B1 are expanded on the right. C, Effect of BFc inputs on principal BLA neuron firing tonically at 2–4 Hz due to a steady depolarizing current (60 pA). Light-induced activation of BFc inputs causes an IPSP and associated firing pause (C1, single 5 ms light stimulus, 6 superimposed trials; C2, 1.5 s train of 5 ms light stimuli at 10 Hz, 6 superimposed trials). C3, Peristimulus histogram of neuronal discharges (black and green bars correspond to C1 and C2, respectively). The 10 Hz train (green) elicits a longer firing pause than the single light stimulus (black). D, Principal BLA neuron made to fire at a high rate by current injection in the absence (black) or presence (blue) of BFc inputs (10 Hz train of 5 ms light stimuli for the duration of the current pulse). At the end of the current pulse, a long-lasting ADP (arrowheads) is observed.
Physiological properties and BFc responsiveness of LF and FS interneurons
Consistent with prior findings (Spampanato et al., 2011; Mańko et al., 2012), the temporal dynamics of current-evoked spiking were strikingly different in FS (Fig. 2A2) and LF (Fig. 2B2) neurons. In response to juxta-threshold depolarizing current pulses from rest, LF cells displayed a conspicuous delay to firing (Fig. 2B2, middle), which diminished as the amplitude of the current pulse was increased (Fig. 2B2, top). In contrast, FS cells showed little delay to firing in the same testing conditions (Fig. 2A2, middle) and they could sustain high firing rates with little or no spike frequency accommodation (Fig. 2A2, top). An additional distinguishing feature was the duration of their action potentials (measured at half-amplitude: LF, 1.93 ± 0.08 ms; FS, 0.71 ± 0.08 ms; t test, p < 0.0001).
In a few instances, we tested whether LF and FS cells were indeed GABAergic by obtaining simultaneous recordings of synaptically coupled principal cells located in their immediate vicinity. The principal cells were recorded in voltage-clamp mode at a holding potential of −50 mV. Spiking in FS (Fig. 2A3) or LF (Fig. 2B3) cells elicited IPSCs in principal cells (n = 8), confirming that these two cell types are GABAergic interneurons.
Paralleling the differing physiological properties of LF and FS cells, photic activation of BFc inputs produced contrasting effects in these two cell types. Indeed, whereas BFc inputs elicited no detectable response in FS cells, LF neurons exhibited robust EPSPs (Fig. 2B4; 10.9 ± 1.5 mV from rest) whose latencies ranged between 8 and 11 ms. Consistent with a mediation by nicotinic receptors, these EPSPs had an extrapolated reversal potential near 0 mV and were blocked by application of the nicotinic receptor antagonist mecamylamine (10 μm, n = 4; Fig. 2B4, right).
Impact of BFc inputs on principal cells
Principal BLA neurons could be easily distinguished from interneurons based on the dynamics of current-evoked firing and morphology. Indeed, principal cells displayed spike frequency accommodation (Fig. 3A) and their dendrites were densely covered with spines (Fig. 3B). The latter property could be observed with post hoc biocytin revelation (Fig. 3B1) and during the recordings with Alexa 594 (Fig. 3B2).
We investigated the effects of BFc inputs on principal cells in two activity regimes: during quiescence (hereafter termed subthreshold effects) or when current injection elicited sustained firing (hereafter termed suprathreshold effects). Unexpectedly, light-induced activation of BFc inputs produced effects of opposite polarity in these two conditions. At rest, the predominant response was a long-lasting hyperpolarization (0.48 ± 0.06 s, n = 67; Fig. 3C1). When the cells were made to fire at modest rates (<8 Hz) by injection of steady depolarizing current, activation of BFc inputs transiently silenced the cells (Fig. 3C) and the duration of this pause increased with the duration of the light stimulus (one light pulse, 2.48 ± 0.18 s; Fig. 3C1,C3; 1.5 s light pulse train at 10 Hz, 4.07 ± 0.24 s; Fig. 3C2,C3; paired t test, p = 0.01). In contrast, when the cells were strongly activated by current injection (firing rate > 8 Hz), the light stimuli had a marginal effect on their firing rate during the current pulse but produced prolonged ADPs (Fig. 3D). The contrasting effects of BFc inputs in these two activity regimes are considered in detail below, first using whole-cell and then perforated patch recordings. Figure 4 presents population analyses that contrast the results obtained with these two recording configurations. Unless otherwise indicated, we used single 5 ms pulses of blue light to analyze the impact of BFc inputs in the subthreshold regime and 1.5 s trains of light stimuli (each 5 ms long, delivered at 10 Hz) for the suprathreshold regime.
Incidence and properties of optically evoked cholinergic IPSPs and ADPs. A, Proportion of recorded cells in which optical activation of BFc axons elicited a late IPSP (black) or ADP (red) using the whole-cell (n = 51) or perforated-patch recording (n = 16) methods. B, Duration and amplitude (C) of BFc-evoked late IPSPs (black) or ADPs (red) using the whole-cell or perforated-patch recording method (averages ± SEM). ADP amplitudes were significantly higher with the perforated-patch method (t test, p = 0.008). Otherwise, the two recording methods yielded similar results for both ADPs and late IPSPs.
Subthreshold effects
In the subthreshold regime, our analyses revealed that the BFc-evoked inhibition was in fact comprised of two distinct components that peaked at different times, reversed at different potentials, and exhibited a contrasting pharmacological sensitivity (Fig. 5). Indeed, activation of BFc inputs first elicited an early IPSP (Fig. 5A, square; latency to peak, 36.9 ± 3.1 ms; onset latencies, 16–20 ms) of short duration (40.1 ± 2.4 ms) that reversed in polarity positive to −70 mV (Fig. 5A3; −57.6 ± 3.7 mV), close to the theoretical Cl− reversal potential with our recording solutions. This early component was followed by an IPSP of higher amplitude (Fig. 5A, circle; 7.7 ± 1.6 mV from −55 mV) and duration (0.68 ± 0.09 s) that reversed negative to −85 mV (Fig. 5A3). It should be noted that no washout of the late IPSP was noticed during whole-cell recordings (Fig. 5B2). Thus, results obtained in the whole-cell (n = 51) and perforated-patch recording (n = 16) modes are pooled below.
Mechanisms underlying inhibitory response of principal neurons to BFc inputs. A–D, Four different principal BLA neurons. A, Light-induced activation of BFc inputs (blue line, 5 ms pulse) elicits an IPSP comprised of two components: an early one, marked by a black square, and late one, marked by a red circle. The early component is shown with an expanded time base in A2. As the prestimulus membrane potential is modified (numbers on the left), the early component (square) reverses at a more depolarized potential than the late component (A3). B1, BFc-evoked late IPSP (top, control) is largely reduced by atropine (middle) whereas the early IPSP (reversed) is sensitive to mecamylamine (bottom). B2, Amplitude of early (black) and late (red) IPSPs (y-axis) plotted as a function of time (x-axis) in control conditions, in the presence of atropine (middle) or atropine and mecamylamine (right). Same cell as in B1. C, Late IPSP component is nearly abolished by pirenzepine. D, Late IPSP is not significantly affected by CGP-55845, apamin, or MTA. E, Average (±SEM) amplitude of BFc-evoked late IPSP in control conditions (black) and in the presence of various drugs. Significant reductions are marked by asterisks (paired t tests, p ≤ 0.001).
Paralleling their contrasting time course and voltage dependence, the two components exhibited a differential sensitivity to nicotinic and muscarinic receptor antagonists (Fig. 5B–E). Indeed, the early IPSP was insensitive to atropine (1.5 μm; n = 10), but was largely reduced by mecamylamine (10 μm; n = 3; Fig. 5B) and picrotoxin (100 μm; n = 5). Conversely, the late IPSP was nearly abolished by atropine (Fig. 5B; n = 10, t = −3.72, p < 0.001) and the M1 receptor antagonist pirenzepine (Fig. 5C,E; 1 μm, n = 4; t = −15.73, p < 0.01), but it persisted in the presence of the M2 receptor antagonist methoctramine (MTA; Fig. 5D,E; 1 μm, n = 3).
The late IPSP was also largely reduced by the inwardly rectifying K+ conductance blocker barium (Fig. 5E; n = 5, t = 7.94, p < 0.001), but was insensitive to picrotoxin (100 μm, n = 5) and the GABA-B antagonist CGP-55845 (Fig. 5D; 10 μm, n = 5), as well as ionotropic glutamate receptor antagonists (CNQX, 20 μm; AP-5, 100 μm; n = 10). Because a prior study reported that muscarinic receptor activation could elicit an IPSP in principal cells via the activation of SK channels secondary to Ca2+ release from intracellular stores (Power and Sah, 2008), we tested whether the late IPSP was sensitive to the SK antagonist apamin. It was not (Fig. 5D,E; 100 nm, n = 3). However, we note that in this prior study, the IPSP developed when the muscarinic agonist was applied while firing the cells (Power and Sah, 2008).
These results suggest that in principal BLA neurons at rest, BFc inputs exert an inhibitory effect. This inhibition is comprised of two phases. The first component is likely mediated by the nicotinic activation of GABAergic local-circuit cells, possibly LF cells (Fig. 2B4), which then elicit GABA-A IPSPs in principal neurons. This is supported by the sensitivity of the early IPSPs to mecamylamine and picrotoxin and the fact that the IPSP latency in principal cells (∼18 ms) was longer than that of the nicotinic EPSPs in LF cells (∼10 ms). In contrast, the second component results from the direct activation of M1 receptors on principal cells and the consequent activation of an inwardly rectifying K+ conductance.
Finally, we sought to determine whether longer lasting stimulation protocols (8–20 s trains of light pulses at 4–20 Hz) would elicit the slow excitation previously observed in pharmacological experiments (Cole and Nicoll, 1984; Benson, 1988). While the muscarinic IPSP was observed in all principal cells tested with this stimulation protocol (n = 6), no depolarization could be observed, even when the cells were tonically depolarized just below spike threshold with DC current injection.
Suprathreshold effects
We first describe the main properties of the ADPs and then consider their pharmacological sensitivity. From rest, ADPs could be elicited in all tested principal cells by pairing suprathreshold intracellular current injections with cholinergic stimulation (n = 64; 10 Hz train of 5 ms blue light pulses for the duration of the current injection). In these conditions, ADP amplitudes and durations were variable, ranging between 2 and 14 mV and 7–10 s, respectively (Figs. 1D, 6, 7). Importantly, in whole-cell recording mode, ADP amplitudes decreased rapidly with time (50–85% reduction in 10 min; Fig. 6A). Thus, the following analyses are based on perforated-patch recordings of principal cells (n = 16). In these conditions, peak ADP amplitudes and durations averaged 5.1 ± 3.3 mV and 8.3 ± 2.9 s, respectively.
Properties of BFc-evoked ADP. A, Conventional patch recording. Response of BLA neuron to same depolarizing current pulse applied from rest (−65 mV), 1 min, or 10 min after recording onset. Note nearly complete disappearance of ADP at the later time point. Apamin (100 nm) was present throughout. B–E, Different BLA neurons recorded with the perforated-patch method. B, Response of the same BLA neuron to depolarizing current pulses of gradually increasing amplitude in the absence (B1, black) or presence (B2, blue) of blue light (10 Hz train of 5 ms stimuli during current pulse). ADP is only present with light stimuli. B3, Expanded overlay (200 pA current pulse). C, Variations in ADP amplitude as a function of firing rate during depolarizing current pulse. Left, Three superimposed trials with current pulses of different amplitudes (color coded). Right, ADP amplitude (y-axis) as a function of firing frequency (x-axis) during current pulses for cell shown on the left. D, ADP amplitude (y-axis) as a function of firing frequency (x-axis) during depolarizing current pulses for all cells recorded with the perforated-patch method. E, ADP elicits firing when depolarizing current pulses are applied from −60 mV. From left to right, current pulse amplitude increases in steps of 20 pA from 60 to 140 pA.
Pharmacological sensitivity of BFc-evoked ADP. A–C, Top, Grouped data indicating means (± SEM). Bottom, Individual examples. In all cases, black indicates control response with light stimulus in the absence of drug. Red indicates response with light stimulus in the presence of the tested drug. All tests from rest with depolarizing current pulses eliciting firing at ∼10–14 Hz. Voltage responses to current pulses are truncated. The three parts, respectively, show the effect of atropine (A; 1.5 μm), pirenzepine (B; 1 μm), and flufenamic acid (C; 100 μm). ADP was measured as voltage difference between peak voltage within 4 s of current pulse offset and prepulse value. Significant reductions are marked by asterisks (paired t tests, p ≤ 0.001).
ADPs could not be elicited in the absence of cholinergic stimulation, no matter how much depolarizing current was injected (Fig. 6B1). They were only seen with concurrent current-evoked firing and BFc stimulation (Fig. 6B2). Conversely, when cholinergic stimulation was applied in the absence of suprathreshold depolarizing current injection, no depolarization was seen. Also, ADP amplitudes did not vary linearly with the amount of depolarizing current injected or with firing frequency (Fig. 6C,D). Instead, we observed an inverted U relationship such that ADP amplitudes grew when current-evoked firing rates increased up to ∼10–18 Hz and then decreased with further increases in discharge rates (Fig. 6D; polynomial fit with two terms; p = 0.0092 and p = 0.019). Finally, while ADPs generally did not trigger spikes when elicited from rest (2 of 16 cells), they always did when the prepulse potential was set positive to −60 mV by intracellular current injection (Fig. 6E).
The pharmacological sensitivity of ADPs was investigated in the presence of CNQX (20 μm), AP-5 (100 μm), and picrotoxin (100 μm). As shown in Figure 7, ADPs were sensitive to the nonselective muscarinic receptor antagonist atropine (1 μm; n = 5; Fig. 7A), the selective M1 receptor antagonist pirenzepine (1 μm; n = 5; Fig. 7B), and the ICAN (Ca2+-activated nonselective cation channel) blocker flufenamic acid (100 μm; n = 5; Fig. 7C). In contrast, the M2 receptor antagonist MTA (1 μm) had no effect (n = 3). In light of prior findings (Yajeya et al., 1997, 1999; Haj-Dahmane and Andrade, 1999; Egorov et al., 2006), these results suggest that ADPs are mediated through the activation of ICAN secondary to M1 receptor signaling during endogenous ACh release.
Effect of local pressure application of carbachol
It is possible that the discrepancy between the cholinergic effects observed here and prior pharmacological studies is not due to differences between the stimulation methods but to the particular mouse strain or experimental conditions we used. To determine whether this was the case, in the same conditions and mice as above, we examined the effects of CCh (3 μm or 10 μm) in 23 principal BL neurons in the presence of CNQX (20 μm), AP-5 (100 μm), and picrotoxin (100 μm). CCh was either added to the perfusate (n = 10) or delivered by local pressure application (n = 13).
As mentioned above and documented in Figure 4, using optogenetic methods, the muscarinic IPSP and ADPs were seen in virtually all tested cells (≥96%). In contrast, using either pressure application of CCh or addition of the drug to the perfusate, muscarinic IPSPs were never observed. A representative example of response evoked by pressure-applied CCh is shown in Figure 8A, along with group statistics (Fig. 8B). In all tested cells and regardless of the CCh concentration (3 μm or 10 μm) or method of drug application, CCh elicited a long-duration depolarization that was associated with an increase in input resistance. With pressure-applied CCh (10 μm), we noticed that the increased input resistance outlasted the EPSP by >1.5 min, indicating the recruitment of several parallel mechanisms by CCh. When CCh (10 μm) was applied via the perfusate, the response peaked within 13.3 ± 1.5 s at 11.2 ± 2.6 mV, and continued for as long as the CCh application persisted. Whereas pirenzepine (1 μm; n = 5) blocked all CCh effects, flufenamic acid only reduced the EPSP by 52 ± 15% (n = 3).
Effect of CCh on principal BLA neurons. All tests were performed in the presence of CNQX (20 μm), AP-5 (100 μm), and picrotoxin (100 μm). A, Pressure-applied CCh (10 μm) elicits slow EPSP in principal cell at rest (−69 mV). A negative current pulse of fixed amplitude (100 pA) was applied every 5 s to monitor input resistance. B, Averages ± SEM of CCh-evoked EPSP amplitudes (B1) and input resistance changes (B2) elicited by 3 μm (gray) and 10 μm (black) CCh. Sample sizes are indicated below. C, Principal cell made to fire by injection of steady depolarizing current (15 pA) shows firing rate acceleration during and shortly after pressure application of CCh (10 μm).
To rule out the possibility that the lack of CCh-evoked IPSP was due to the fact that test potentials were too hyperpolarized, in eight principal cells we injected steady depolarizing currents that elicited firing at ∼0.3–0.5 Hz (0.39 ± 0.08 Hz) and then pressure applied CCh. CCh (10 μm) produced a significant increase in firing rate (to 1.15 ± 0.16 Hz; paired t test, p = 0.002), but again no IPSP.
Discussion
We investigated the influence of BFc inputs on BLA neurons using optogenetic methods. The interest of this question stems from earlier observations suggesting that ACh facilitates memory formation in BLA-dependent tasks. We found that BFc inputs exert cell type-specific effects that also depend on the state of target neurons. In particular, select subtypes of interneurons (LF but not FS cells) exhibited fast nicotinic EPSPs that led to the generation of GABA-A IPSPs in principal cells. In addition to eliciting this rapid inhibition, BFc inputs evoked a long-lasting IPSP in principal cells, resulting from the direct activation of M1 receptors and the consequent activation of an inwardly rectifying K+ conductance. In addition to these inhibitory effects, however, BFc inputs could excite principal cells provided they coincided with a critical level of depolarization. In these conditions, BFc inputs elicited a prolonged M1-mediated ADP. Below, we consider the significance of these findings in light of earlier work about cholinergic influences in the BLA and cortex, as well as their impact on memory formation.
Prior findings on the influence of ACh
Most prior studies on cholinergic influences in cortex and BLA relied on prolonged applications of cholinergic agonists in vitro. Using this approach, it was generally found that cholinergic agonists produce an excitation of principal BLA (Womble and Moises, 1993; Egorov et al., 2006) and cortical (for review, see McCormick, 1993) neurons via the activation of muscarinic receptors. In the amygdala, depending on the particular study, this effect was found to depend on Ca2+ entry and the consequent activation of ICAN (Yajeya et al., 1997, 1999; Egorov et al., 2006) or, more commonly, on the inhibition of various K+ conductances leading to the generation of slow EPSPs associated with increased input resistances (Washburn and Moises, 1992; Womble and Moises, 1992, 1993; Pape and Driesang, 1998; Faber and Sah, 2002). However, in principal amygdala (Power and Sah, 2008) and cortical (Gulledge and Stuart, 2005; Gulledge et al., 2007, 2009) neurons, it was also reported that muscarinic receptor activation could have a direct inhibitory effect via the activation of SK channels. However, this inhibitory effect was dependent on prior Ca2+ entry following neuronal spiking in amygdala neurons versus Ca2+ release from IP3-sensitive intracellular Ca2+ stores in cortical cells (Power and Sah, 2008).
Here, we found that optogenetic activation of BFc inputs to BLA neurons does not trigger the canonical, slow cholinergic EPSP documented in prior pharmacological studies. Moreover, the muscarinic IPSPs observed in our study did not require SK channels. Paralleling this situation, opposite conclusions were also reached regarding the cholinergic responsiveness of olfactory bulb (OB) neurons when using pharmacological versus optogenetic methods. Indeed, Smith and Araneda (2010), using a pharmacological approach, found an increased M1 receptor-mediated excitability in granule and mitral/tufted OB neurons. In contrast, delivery of endogenous ACh with optogenetic activation of BFc axons in vivo completely silenced these OB neurons (Ma and Luo, 2012).
A likely explanation for these divergent results resides in the contrasting spatiotemporal profile of cholinergic receptor activation produced by adding drugs to the perfusate versus activating BFc axons. Indeed, whereas the former method causes a prolonged and spatially uniform concentration of cholinergic agonists, the latter leads to a transient and spatially heterogeneous rise in ACh concentration. In particular, optogenetic activation of BFc inputs is expected to produce regions of higher ACh concentration at proximity of cholinergic axon terminals and a lower activation of extrasynaptic receptors than the pharmacological approach. A counterargument to this proposal is the long-held position that most cholinergic synapses lack conventional postsynaptic specializations necessary for the emergence of functional ACh microdomains (Umbriaco et al., 1994; Mrzljak et al., 1995; Yamasaki et al., 2010). However, we note that other electron microscopic investigations from highly respected laboratories support the opposite view (Beaulieu and Somogyi, 1991; Turrini et al., 2001; Muller et al., 2011, 2013).
These considerations suggest that the divergent results of pharmacological and optogenetic studies result from the fact that the former approach produces more activation of extrasynaptic receptors. Implicit in this formulation is the possibility that the downstream effectors of synaptic and extrasynaptic M1 receptors are different, a proposal that awaits experimental scrutiny.
The impact of BFc inputs depends on the state of principal BLA neurons
As observed in the present study, prior optogenetic studies in the striatum (English et al., 2012) and cortex (Arroyo et al., 2012; Bennett et al., 2012) revealed that optogenetic activation of cholinergic axons elicits nicotinic EPSPs in GABAergic interneurons, and subsequently GABA-A IPSPs in principal cells. In cortex, select subtypes of interneurons exhibit these nicotinic EPSPs (Arroyo et al., 2012): as observed here, LF cells do but FS cells do not.
However, to the best of our knowledge, prior optogenetic studies did not investigate the neuromodulatory actions of ACh produced via the activation of metabotropic receptors. Indeed, we found that in principal BLA cells, the fast GABA-A IPSPs resulting from the nicotinic activation of interneurons were followed by a prolonged IPSP that reversed near the K+ equilibrium potential. These long-lasting IPSPs were sensitive to atropine, pirenzepine, and barium suggesting that they are mainly mediated by M1 receptor activation coupled to the activation of G-protein-coupled inward rectifier channels (Eggermann and Feldmeyer, 2009).
Although BFc inputs only exerted inhibitory effects in quiescent principal neurons, this was not the case when activation of BFc inputs was paired to strong activation of the target cells. In these conditions, the muscarinic inhibition appeared to be overwhelmed and excitation predominated. This excitation took the form of prolonged (7–10 s) ADPs, which invariably elicited firing from potentials positive to −60 mV, but typically not from more polarized potentials. Pharmacological analyses revealed that these ADPs were dependent on M1 receptor activation and that they were sensitive to flufenamic acid. In light of prior findings in cortex (Haj-Dahmane and Andrade, 1999) and amygdala (Yajeya et al., 1997, 1999; Egorov et al., 2006), these results suggest that the BFc-evoked ADPs depend on an M1-mediated activation of ICAN. Here, it should be noted that while a muscarinic activation of ICAN was previously reported in principal BLA neurons (Yajeya et al., 1997, 1999), in contrast to the present study, no prior depolarization was required with pharmacological activation of muscarinic receptors.
Possible implication for cholinergic influences over BLA-dependent memory tasks
As reviewed in the Introduction, much evidence indicates that cholinergic inputs to the amygdala facilitate memory formation in BLA-dependent tasks. Yet, the mechanisms underlying this effect remain unclear. The results of the present study shed new light on this question. Indeed, we observed that the impact of BFc inputs on principal cells varies depending on how strongly activated they are. Weakly activated cells are inhibited by BFc inputs whereas strongly activated neurons are further excited, in effect increasing differentiation of activity levels among principal cells. These findings integrate well into the notion of an acetylcholine-mediated increase in signal/noise ratio in cortical structures, proposed earlier by other authors (Sarter et al., 2005; Giocomo and Hasselmo, 2007). Given earlier findings indicating that a form of synaptic competition governs the assignment of principal neurons to memory traces (Han et al., 2007, 2009) and evidence that disynaptic inhibition between principal cells plays a critical role in this respect (Kim et al., 2013), our results suggest that BFc inputs might be facilitating memory formation by biasing the synaptic competition in favor of the strongly activated cells. Also, because the cholinergic ADPs lengthen the effective period of excitation, they might contribute to the formation of stimulus associations during conditioning tasks where the timing of conditioned and unconditioned stimuli is not optimal for the induction of synaptic plasticity.
Footnotes
This work was supported by National Institutes of Health Grants 23945 to L.Z. and MH-098738 to D.P.
The authors declare no competing financial interests.
- Correspondence should be addressed to Laszlo Zaborszky, Center for Molecular and Behavioral Neuroscience, Rutgers, The State University, 197 University Avenue, Newark, NJ 07102. laszloz{at}andromeda.rutgers.edu
