Figure 1. caMEK5-eGFP is conditionally induced in the SVZ and OB regions of adult brain in NesCreER:caMEK5 mice upon tamoxifen administration. A, Experimental scheme for generating NesCreER:caMEK5 mice. Nestin-CreERTM:caMEK5-eGFPloxP/loxP mice were generated by crossing (ROSA26)caMEK5-eGFPloxP/loxP with Nestin-CreERTM mice. Eight-week-old mice were treated with vehicle (corn oil) or tamoxifen by oral gavaging once a day for 7 d and perfused 3 weeks after the last tamoxifen dosing. B–I, Anti-eGFP immunostaining (green) shows the expression of caMEK5-eGFP in the SVZ of the lateral ventricle and the olfactory bulb of NesCreER:caMEK5 (D, E, H, I) but not the vehicle-treated control mice (B, C, F, G). Scale bars: D, H, 100 μm; for panels of SVZ and OB images, respectively. J–U, eGFP is not expressed in other regions of the adult brain including the cortex (J, K), striatum (L, M), CA1 (N, O), CA3 (P, Q), thalamus (R, S), and hypothalamus (T, U). Scale bars: J–U, 50 μm.