Figure 2. Evoked −astrocyte EPSCs, but not IPSCs, are desynchronized. A, Representative PSCs of GABAergic (left) and glutamatergic autaptic neurons (right) grown on +astrocyte microcultures (black traces, n = 9 and 12, respectively) or −astrocyte microcultures (gray IPSCs, n = 10; red EPSCs, n = 14). The bar graph summarizes differences in PSC waveforms between +astrocyte (black dotted line) and −astrocyte (gray and red bars, respectively) PSCs. −Astrocyte PSCs are normalized to +astrocyte PSC values. B, Representative traces of +astrocyte (black trace, n = 13) and −astrocyte (red trace, n = 17) EPSCs, focusing on synaptic delay between presynaptic stimulation and postsynaptic response (inset). C, Representative EPSCs from A, with asterisks denoting local minima in each trace (see Materials and Methods). D, Averaged local peak count and synaptic delay for +astrocyte and −astrocyte conditions displayed in bar graph (+astrocytes, n = 12; −astrocytes, n = 13). E, Two sequential evoked EPSCs from a −astrocyte glutamatergic neuron, separated by 25 s. Asterisks indicate local peaks detected via local peak count analysis, with color matched to trace color. F, Three evoked EPSCs recorded from +astrocyte glutamatergic neuron with 25 s between each stimulation. The boxed inset magnifies examples of quantal asynchrony occurring after the evoked response. *p < 0.05.