Article Information
- Received May 3, 2015
- Revision received August 17, 2015
- Accepted August 21, 2015
- First published September 30, 2015.
- Version of record published September 30, 2015.
Author Information
- Yoshiyasu Fukusumi1,*,
- Florian Meier1,*,
- Sebastian Götz1,*,
- Friederike Matheus1,
- Martin Irmler2,
- Ruth Beckervordersandforth3,
- Theresa Faus-Kessler1,
- Eleonora Minina1,
- Benedict Rauser1,
- Jingzhong Zhang1,
- Ernest Arenas4,
- Elisabet Andersson5,
- Christof Niehrs6,7,
- Johannes Beckers2,8,
- Antonio Simeone9,10,
- Wolfgang Wurst1,11,12,13, and
- Nilima Prakash1,11
- 1Institutes of Developmental Genetics and
- 2Experimental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, D-85764 Neuherberg, Germany,
- 3Emil Fischer Centre, Institute of Biochemistry, Friedrich Alexander University Erlangen-Nuremberg, D-91054 Erlangen, Germany,
- 4Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics and
- 5Department of Cell and Molecular Biology, Karolinska Institute, S-17177 Stockholm, Sweden,
- 6Division of Molecular Embryology, German Cancer Research Center–Center for Molecular Biology at Heidelberg University Alliance, German Cancer Research Center, D-69120 Heidelberg, Germany,
- 7Institute of Molecular Biology, D-55128 Mainz, Germany,
- 8Technical University Munich, Chair of Experimental Genetics, D-85350 Freising-Weihenstephan, Germany,
- 9Institute of Genetics and Biophysics “A. Buzzati-Traverso,” I-80131 Naples, Italy,
- 10Institute for Inpatient Treatment and Scientific Studies Neuromed, I-86077 Pozzilli, Isernia, Italy,
- 11Technical University Munich, Chair of Developmental Genetics/Helmholtz Center Munich, D-85764 Neuherberg, Germany,
- 12German Center for Neurodegenerative Diseases, D-80336 Munich, Germany, and
- 13Munich Cluster for Systems Neurology, Adolf Butenandt Institute, Ludwig Maximilians University Munich, D-80336 Munich, Germany
Author contributions
Author contributions: W.W. and N.P. designed research; Y.F., F.Me., S.G., F.Ma., M.I., R.B., E.M., and B.R. performed research; J.Z., E.An., and C.N. contributed unpublished reagents/analytic tools; Y.F., F.Me., S.G., M.I., R.B., T.F.-K., E.Ar., J.B., A.S., and N.P. analyzed data; N.P. wrote the paper.
↵*Y.F., F.Me., and S.G. contributed equally to this work.
Disclosures
- Received May 3, 2015.
- Revision received August 17, 2015.
- Accepted August 21, 2015.
This work was supported by Italian Association for Cancer Research Grant IG2013 (A.S.), the German Research Foundation within the framework of the Munich Cluster for Systems Neurology (EXC 1010 SyNergy), the Bavarian State Ministry for Education and Culture, Science, and Art within the Bavarian Research Network “Human Induced Pluripotent Stem Cells,” and the Systems Biology of Stem Cells and Reprogramming Project, which received funding from the European Union Seventh Framework Programme for Research, Technological Development, and Demonstration under Grant Agreement FP7-HEALTH-F4-2010-242129 (W.W.). We are particularly grateful to Prof. C. Birchmeier-Kohler and Dr. T. Müller for kindly providing the LMX1B antibody and to Prof. M. Li for providing the Pitx3GFP/+ mice. We thank Dr. M. Endele for help with the FACS of VM cells and A. Bettenbrock, A. Folchert, and S. Badeke for excellent technical assistance. The monoclonal En1 antibody was obtained through the Developmental Studies Hybridoma Bank under the auspices of the National Institute of Child Health and Human Development and maintained by the University of Iowa (Iowa City, IA).
The authors declare no competing financial interests.
- Correspondence should be addressed to either of the following: Wolfgang Wurst, Institute of Developmental Genetics, Helmholtz Center Munich, German Research Center for Environmental Health, and Technical University Munich, Chair of Developmental Genetics, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany, wurst{at}helmholtz-muenchen.de; or Nilima Prakash, Hamm-Lippstadt University of Applied Sciences, Marker Allee 76-78, D-59063 Hamm, Germany, nilima.prakash{at}hshl.de
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