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Cover legend: This is a composite image of a rat hippocampal neuron expressing mCherry (red, confocal) and the actin-binding fluorescent peptide LifeAct-Venus (pseudocolors, CW-STED). STED imaging of LifeAct-Venus and post hoc deconvolution allows nanoscopic investigations of changes in cytoskeleton organization within dendritic spines. For more information, see the article by Chevy et al. (pages 15772–15786).