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Stimulation-Evoked Ca2+ Signals in Astrocytic Processes at Hippocampal CA3–CA1 Synapses of Adult Mice Are Modulated by Glutamate and ATP

Wannan Tang, Karolina Szokol, Vidar Jensen, Rune Enger, Chintan A. Trivedi, Øivind Hvalby, P. Johannes Helm, Loren L. Looger, Rolf Sprengel and Erlend A. Nagelhus
Journal of Neuroscience 18 February 2015, 35 (7) 3016-3021; https://doi.org/10.1523/JNEUROSCI.3319-14.2015
Wannan Tang
1Centre for Molecular Medicine Norway, The Nordic EMBL Partnership,
3Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway,
4Department of Molecular Neurobiology and
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Karolina Szokol
1Centre for Molecular Medicine Norway, The Nordic EMBL Partnership,
3Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway,
6Oslo University Hospital, Department of Neurology, 0027 Oslo, Norway,
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Vidar Jensen
1Centre for Molecular Medicine Norway, The Nordic EMBL Partnership,
3Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway,
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Rune Enger
1Centre for Molecular Medicine Norway, The Nordic EMBL Partnership,
3Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway,
6Oslo University Hospital, Department of Neurology, 0027 Oslo, Norway,
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Chintan A. Trivedi
5Neural Circuits and Behaviour Research Group, Department of Biomedical Optics, Max Planck Institute for Medical Research, D-69120 Heidelberg, Germany, and
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Øivind Hvalby
2Department of Physiology, and
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P. Johannes Helm
3Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway,
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Loren L. Looger
7Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 20147, and
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Rolf Sprengel
4Department of Molecular Neurobiology and
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Erlend A. Nagelhus
1Centre for Molecular Medicine Norway, The Nordic EMBL Partnership,
3Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway,
6Oslo University Hospital, Department of Neurology, 0027 Oslo, Norway,
8Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center, Rochester, New York 14618
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.3319-14.2015
PubMed 
25698739
Published By 
Society for Neuroscience
History 
  • Received August 10, 2014
  • Revision received January 6, 2015
  • Accepted January 11, 2015
  • First published February 18, 2015.
  • Version of record published February 18, 2015.
Copyright & Usage 
Copyright © 2015 the authors 0270-6474/15/353016-06$15.00/0 This article is freely available online through the J Neurosci Author Open Choice option.

Author Information

  1. Wannan Tang1,3,4,
  2. Karolina Szokol1,3,6,
  3. Vidar Jensen1,3,
  4. Rune Enger1,3,6,
  5. Chintan A. Trivedi5,
  6. Øivind Hvalby2,
  7. P. Johannes Helm3,
  8. Loren L. Looger7,
  9. Rolf Sprengel4, and
  10. Erlend A. Nagelhus1,3,6,8
  1. 1Centre for Molecular Medicine Norway, The Nordic EMBL Partnership,
  2. 2Department of Physiology, and
  3. 3Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway,
  4. 4Department of Molecular Neurobiology and
  5. 5Neural Circuits and Behaviour Research Group, Department of Biomedical Optics, Max Planck Institute for Medical Research, D-69120 Heidelberg, Germany, and
  6. 6Oslo University Hospital, Department of Neurology, 0027 Oslo, Norway,
  7. 7Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 20147, and
  8. 8Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center, Rochester, New York 14618
View Full Text

Author contributions

  1. Author contributions: W.T., K.S., V.J., R.E., O.H., P.J.H., R.S., and E.A.N. designed research; W.T., K.S., V.J., R.E., and C.A.T. performed research; L.L.L. contributed unpublished reagents/analytic tools; W.T., K.S., and R.E. analyzed data; W.T., R.E., O.H., L.L.L., and E.A.N. wrote the paper.

View Abstract

Disclosures

    • Received August 10, 2014.
    • Revision received January 6, 2015.
    • Accepted January 11, 2015.
  • This work was supported by a European Molecular Biology Organization short-term fellowship (W.T.), the German Academic Exchange Service (DAAD) mobility programme Germany–Norway (R.S. and E.A.N.), the Research Council of Norway (E.A.N.), the European Union Seventh Framework Programme for Research, Technological Development, and Demonstration under Grant Agreement 601055, and the Letten Foundation. We thank Prof. Petter Laake (Department of Biostatistics, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway) for advice on the statistical analysis.

  • The authors declare no competing financial interests.

  • Correspondence should be addressed to either Wannan Tang or Erlend A. Nagelhus, Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway. wannan.tang{at}medisin.uio.no, e.a.nagelhus{at}medisin.uio.no

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Jan 2018203711
Feb 201892618
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Apr 201899720
May 201812686
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Jul 2018106010
Aug 20184379
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Aug 2025306015
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Oct 2025205416
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Total 2025231680152
Total80245421187
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The Journal of Neuroscience: 35 (7)
Journal of Neuroscience
Vol. 35, Issue 7
18 Feb 2015
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Stimulation-Evoked Ca2+ Signals in Astrocytic Processes at Hippocampal CA3–CA1 Synapses of Adult Mice Are Modulated by Glutamate and ATP
Wannan Tang, Karolina Szokol, Vidar Jensen, Rune Enger, Chintan A. Trivedi, Øivind Hvalby, P. Johannes Helm, Loren L. Looger, Rolf Sprengel, Erlend A. Nagelhus
Journal of Neuroscience 18 February 2015, 35 (7) 3016-3021; DOI: 10.1523/JNEUROSCI.3319-14.2015

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Stimulation-Evoked Ca2+ Signals in Astrocytic Processes at Hippocampal CA3–CA1 Synapses of Adult Mice Are Modulated by Glutamate and ATP
Wannan Tang, Karolina Szokol, Vidar Jensen, Rune Enger, Chintan A. Trivedi, Øivind Hvalby, P. Johannes Helm, Loren L. Looger, Rolf Sprengel, Erlend A. Nagelhus
Journal of Neuroscience 18 February 2015, 35 (7) 3016-3021; DOI: 10.1523/JNEUROSCI.3319-14.2015
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Keywords

  • astrocytes
  • endfeet
  • GCaMP
  • glia
  • hippocampus
  • two-photon

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